Characterisation of DRASIC in the mouse inner ear

Hildebrand, Michael S.; Silva, Michelle G. de; Klockars, Tuomas; Rose, Elizabeth; Price, Margaret P.; Smith, Richard J.H.; McGuirt, Wyman T.; Christopoulos, Helen; Petit, Christine; Dahl, Hans‐Henrik M.

doi:10.1016/s0378-5955(04)00015-2

Cited by 62 publications

(56 citation statements)

References 25 publications

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“…Both the ASIC3 transcript and protein were found to be enriched in the cochlea (78). ASIC3 expression was shown in cells and neural fibers of the spiral ganglion as well as cells of the organ of Corti (78). In addition to the normal mouse ASIC3 cDNA, an alternatively spliced transcript was elucidated by RT-PCR from the mouse inner ear (78).…”

Section: Roles In Mechanosensationmentioning

confidence: 99%

“…ASIC3 expression was shown in cells and neural fibers of the spiral ganglion as well as cells of the organ of Corti (78). In addition to the normal mouse ASIC3 cDNA, an alternatively spliced transcript was elucidated by RT-PCR from the mouse inner ear (78). This transcript may represent a new isoform with an as yet unknown function.…”

Section: Roles In Mechanosensationmentioning

confidence: 99%

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ASIC3 Channels in Multimodal Sensory Perception

2010

ACS Chem. Neurosci.

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Acid-sensing ion channels (ASICs), which are members of the sodium-selective cation channels belonging to the epithelial sodium channel/degenerin (ENaC/ DEG) family, act as membrane-bound receptors for extracellular protons as well as nonproton ligands. At least five ASIC subunits have been identified in mammalian neurons, which form both homotrimeric and heterotrimeric channels. The highly proton sensitive ASIC3 channels are predominantly distributed in peripheral sensory neurons, correlating with their roles in multimodal sensory perception, including nociception, mechanosensation, and chemosensation. Different from other ASIC subunit composing ion channels, ASIC3 channels can mediate a sustained window current in response to mild extracellular acidosis (pH 7.3-6.7), which often occurs accompanied by many sensory stimuli. Furthermore, recent evidence indicates that the sustained component of ASIC3 currents can be enhanced by nonproton ligands including the endogenous metabolite agmatine. In this review, we first summarize the growing body of evidence for the involvement of ASIC3 channels in multimodal sensory perception and then discuss the potential mechanisms underlying ASIC3 activation and mediation of sensory perception, with a special emphasis on its role in nociception. We conclude that ASIC3 activation and modulation by diverse sensory stimuli represent a new avenue for understanding the role of ASIC3 channels in sensory perception. Furthermore, the emerging implications of ASIC3 channels in multiple sensory dysfunctions including nociception allow the development of new pharmacotherapy.

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Section: Roles In Mechanosensationmentioning

confidence: 99%

Section: Roles In Mechanosensationmentioning

confidence: 99%

ASIC3 Channels in Multimodal Sensory Perception

2010

ACS Chem. Neurosci.

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“…Inactivation of the ASIC2 gene in mouse also sensitizes the retina toward light-induced degeneration (34). ASIC1, ASIC2, and ASIC3 are also expressed in the mouse inner ear (37,38). ASIC2 is mainly expressed in spiral ganglion neurons in the adult cochlea.…”

Section: Participation In Other Sensory Modalitiesmentioning

confidence: 99%

Acid-sensing Ion Channels in Sensory Perception

Lingueglia

2007

Journal of Biological Chemistry

260

211

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“…ACCN1, 2, and 3 were present in rodent EST libraries. PCR studies revealed expression in the spiral ganglion and fibers of the mouse inner ear (Hildebrand et al 2004). Functionally, ACCN3 mouse knockouts show an increase in hearing thresholds from 2 to 3 months.…”

Section: Ligand-gated Channelsmentioning

confidence: 99%

Ion Channel Gene Expression in the Inner Ear

Gabashvili

Sokolowski

Morton

et al. 2007

JARO

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The ion channel genome is still being defined despite numerous publications on the subject. The ion channel transcriptome is even more difficult to assess. Using high-throughput computational tools, we surveyed all available inner ear cDNA libraries to identify genes coding for ion channels. We mapped over 100,000 expressed sequence tags (ESTs) derived from human cochlea, mouse organ of Corti, mouse and zebrafish inner ear, and rat vestibular end organs to Homo sapiens, Mus musculus, Danio rerio, and Rattus norvegicus genomes. A survey of EST data alone reveals that at least a third of the ion channel genome is expressed in the inner ear, with highest expression occurring in hair cell-enriched mouse organ of Corti and rat vestibule. Our data and comparisons with other experimental techniques that measure gene expression show that every method has its limitations and does not per se provide a complete coverage of the inner ear ion channelome. In addition, the data show that most genes produce alternative transcripts with the same spectrum across multiple organisms, no ion channel gene variants are unique to the inner ear, and many splice variants have yet to be annotated. Our high-throughput approach offers a qualitative computational and experimental analysis of ion channel genes in inner ear cDNA collections. A lack of data and incomplete gene annotations prevent both rigorous statistical analyses and comparisons of entire ion channelomes derived from different tissues and organisms.

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Characterisation of DRASIC in the mouse inner ear

Cited by 62 publications

References 25 publications

ASIC3 Channels in Multimodal Sensory Perception

ASIC3 Channels in Multimodal Sensory Perception

Acid-sensing Ion Channels in Sensory Perception

Ion Channel Gene Expression in the Inner Ear

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