Characterisation of an SKn-type Dehydrin Promoter from Wheat and Its Responsiveness to Various Abiotic and Biotic Stresses

Zhu, Weining; Zhang, David; Lu, Xuanxuan; Zhang, Linsheng; Yu, Zhenwen; Lv, Hui; Zhang, Hongmei

doi:10.1007/s11105-013-0681-1

Cited by 28 publications

(27 citation statements)

References 70 publications

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“…Many cis -acting elements of DHN promoters in plants respond to one or several environmental signals (Lee et al, 2013; Zhu et al, 2014). These cis -acting elements include ABA-responsive elements (ABREs), drought-responsive elements/C-repeats (DRE/CRT), low-temperature-responsive elements (LTRE) and light-responsive elements.…”

Section: Introductionmentioning

confidence: 99%

Expression of CdDHN4, a Novel YSK2-Type Dehydrin Gene from Bermudagrass, Responses to Drought Stress through the ABA-Dependent Signal Pathway

Fan

Xie

et al. 2017

Front. Plant Sci.

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Dehydrin improves plant resistance to many abiotic stresses. In this study, the expression profiles of a dehydrin gene, CdDHN4 , were estimated under various stresses and abscisic acid (ABA) treatments in two bermudagrasses ( Cynodon dactylon L.): Tifway (drought-tolerant) and C299 (drought-sensitive). The expression of CdDHN4 was up-regulated by high temperatures, low temperatures, drought, salt and ABA. The sensitivity of CdDHN4 to ABA and the expression of CdDHN4 under drought conditions were higher in Tifway than in C299. A 1239-bp fragment, CdDHN4-P, the partial upstream sequence of the CdDHN4 gene, was cloned by genomic walking from Tifway. Bioinformatic analysis showed that the CdDHN4-P sequence possessed features typical of a plant promoter and contained many typical cis elements, including a transcription initiation site, a TATA-box, an ABRE, an MBS, a MYC, an LTRE, a TATC-box and a GT1-motif. Transient expression in tobacco leaves demonstrated that the promoter CdDHN4-P can be activated by ABA, drought and cold. These results indicate that CdDHN4 is regulated by an ABA-dependent signal pathway and that the high sensitivity of CdDHN4 to ABA might be an important mechanism enhancing the drought tolerance of bermudagrass.

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Section: Introductionmentioning

confidence: 99%

Expression of CdDHN4, a Novel YSK2-Type Dehydrin Gene from Bermudagrass, Responses to Drought Stress through the ABA-Dependent Signal Pathway

Fan

Xie

et al. 2017

Front. Plant Sci.

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“…The cis-acting elements in promoters play a crucial role in the regulation of gene transcription [40]. Many DHN genes and their promoters have been identified, and their regulatory pathways have been well discovered [27,41,42]. Analysis of GUS activity of the promoter of CaDHN4 in transgenic Arabidopsis under different stress conditions found that low temperature, NaCl, mannitol, drought, MeJA, SA, and ABA treatments, resulted in intense staining in the transgenic plants, as compared to the control plants.…”

Section: Discussionmentioning

confidence: 99%

CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

Zhang

Liu

et al. 2019

IJMS

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Dehydrins play an important role in improving plant resistance to abiotic stresses. In this study, we isolated a dehydrin gene from pepper (Capsicum annuum L.) leaves, designated as CaDHN4. Sub-cellular localization of CaDHN4 was to be found in the nucleus and membrane. To authenticate the function of CaDHN4 in cold- and salt-stress responses and abscisic acid (ABA) sensitivity, we reduced the CaDHN4 expression using virus-induced gene silencing (VIGS), and overexpressed the CaDHN4 in Arabidopsis. We found that silencing of CaDHN4 reduced the growth of pepper seedlings and CaDHN4-silenced plants exhibited more serious wilting, higher electrolyte leakage, and more accumulation of ROS in the leaves compared to pTRV2:00 plants after cold stress, and lower chlorophyll contents and higher electrolyte leakage compared to pTRV2:00 plants under salt stress. However, CaDHN4-overexpressing Arabidopsis plants had higher seed germination rates and post-germination primary root growth, compared to WT plants under salt stress. In response to cold and salt stresses, the CaDHN4-overexpressed Arabidopsis exhibited lower MDA content, and lower relative electrolyte leakage compared to the WT plants. Under ABA treatments, the fresh weight and germination rates of transgenic plants were higher than WT plants. The transgenic Arabidopsis expressing a CaDHN4 promoter displayed a more intense GUS staining than the normal growth conditions under treatment with hormones including ABA, methyl jasmonate (MeJA), and salicylic acid (SA). Our results suggest that CaDHN4 can protect against cold and salt stresses and decrease ABA sensitivity in Arabidopsis.

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“…Shekhawat et al (2011) showed that MusaDHN-1, a SK 3 -type DHN gene in banana, contributed positively towards drought and salt stress tolerance, and responses to ABA, ethylene, and MeJA. Similarly, SK 3 -type DHN gene in wheat WZY1-2 could be induced by dehydration, ABA, SA, MeJA, indole-3-acetic acid, and GA (Zhu et al, 2014). In another study, DHN expression was investigated in ABA-, SA-, and MeJAtreated leaves, in order to determine whether the response of pepper DHNs under stress conditions is related to these molecules.…”

Section: Interaction With Phytohormonesmentioning

confidence: 99%

“…Yamaguchi-Shinozaki and Shinozaki (2005) stated that cis-acting regulatory elements are key molecules involved in the transcriptional regulation of gene activities controlling many abiotic stresses, hormones, and developmental processes. Many genes of DHNs and their promoter regions have been reported and their regulatory roles are well known (Zhu et al, 2014;Lv et al, 2017). For instance, Zhu et al (2014) reported that the promoter of the SK 3 -type DHN gene, called WZY1-2, contained many elements such as ABRE, LTRE, Gibberellin responsive elements (GARE), Salicylic acid responsiveness (SARE) and TGACG (involved in the MeJA-responsiveness).…”

Section: Signaling Pathways Involved In Dhns Gene Expressionmentioning

confidence: 99%

“…Many genes of DHNs and their promoter regions have been reported and their regulatory roles are well known (Zhu et al, 2014;Lv et al, 2017). For instance, Zhu et al (2014) reported that the promoter of the SK 3 -type DHN gene, called WZY1-2, contained many elements such as ABRE, LTRE, Gibberellin responsive elements (GARE), Salicylic acid responsiveness (SARE) and TGACG (involved in the MeJA-responsiveness). They pointed out that WYZ1-2 can be induced by cold and ABA, and that the cis-regulatory elements could play a critical role in expression of gene under stress.…”

Section: Signaling Pathways Involved In Dhns Gene Expressionmentioning

confidence: 99%

See 1 more Smart Citation

Dehydrins: an overview of current approaches and advancement

Güler¹,

Terzi²

2020

Turk J Bot

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Since plants are exposed to diverse environmental stresses in their natural area, numerous reviews have demonstrated many aspects of dehydrins (DHNs), including structural and functional dynamics, and multiple roles such as membrane protection, cryoprotection of enzymes, chaperone feature, and protection from reactive oxygen species. In this review, we have focused on new information, and other promising and emerging topics of DHNs in plants. This review outlines particularly the potential regulatory mechanisms of DHNs associated with stress tolerance and the role of DHNs in morphological responses of the plants exposed to environmental stresses. Besides the discussion of the signaling pathways involved in DHN expression under abiotic stress, novel aspects about the effect of DHN transcript or the protein accumulation on the tolerance to the stress factors are also presented in transgenic and non-transgenic plants. We hope that this review will help us better understand the role of these impressive proteins in plant stress response mechanisms.

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Characterisation of an SKn-type Dehydrin Promoter from Wheat and Its Responsiveness to Various Abiotic and Biotic Stresses

Cited by 28 publications

References 70 publications

Expression of CdDHN4, a Novel YSK2-Type Dehydrin Gene from Bermudagrass, Responses to Drought Stress through the ABA-Dependent Signal Pathway

Expression of CdDHN4, a Novel YSK2-Type Dehydrin Gene from Bermudagrass, Responses to Drought Stress through the ABA-Dependent Signal Pathway

CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

Dehydrins: an overview of current approaches and advancement

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