Characterisation of an autoreactive conformational epitope on GAD65 recognised by the human monoclonal antibody b78 using a combination of phage display, in vitro mutagenesis and molecular modelling

O'Connor, Karen H; Banga, JP; Darmanin, Connie; El‐Kabbani, Ossama; Mackay, Ian R.; Rowley, Merrill J.

doi:10.1016/j.jaut.2006.02.001

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…The PEVKEK loop was identified in the conformational MICA3 epitope supporting previous data that amino acids 511-531 of GAD65 contribute to this epitope [105]. In another study the MoAb b78 identified a GAD65 conformational epitope [106]. A combination of phage display and molecular modelling has been used to characterise the epitope for the 96/3 MoAb generated against the IA-2 autoantigen, one of the relevant autoantigens in Type 1 diabetes; the contribution of residues implicated in 96/3 binding to serum autoantibodies was investigated [107].…”

Section: Searching T1d-related Epitopessupporting

confidence: 79%

Unravelling autoimmune pathogenesis by screening random peptide libraries with human sera

Fierabracci¹

2009

Immunology Letters

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Section: Searching T1d-related Epitopessupporting

confidence: 79%

Unravelling autoimmune pathogenesis by screening random peptide libraries with human sera

Fierabracci¹

2009

Immunology Letters

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“…The following epitopes are mapped on the surface: DPC, M4 and M5. The epitopes constitutes the GAD 65 contact sites to potential antibodies themenschwerpunkt gastrointestinal tract, anticancer drugs, hematopoietic stem/progenitor cell selection, autoimmunity, substrate specificity of granzyme B, mouse mast cell protease, human digestive protease, neural KCNQ channels, feline herpesvirus-1 thymidine kinase and others, have been determined by homology modelling [75][76][77][78][79][80][81][82][83][84][85][86].…”

Section: Discussionmentioning

confidence: 99%

Homology Modelling: Eine Übersicht über die Methode am Beispiel der Strukturbestimmung vom Diabetes Antigen GAD 65

Wiltgen

Tilz

2009

Wien Med Wochenschr

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This introductory paper describes the basic principles and clinical applications of the protein 3D structure prediction by homology modelling. The paper mainly addresses physicians and medical chemists. Because many proteins are of immediate clinical importance, the determination of their structures is crucial for molecular medicine. In homology modelling, a protein sequence with unknown structure is aligned with sequences of known protein structures. By exploiting structural information from the known configurations, the new structure can be predicted. The necessary condition for successful homology modelling is a sufficient similarity between the protein sequences. Because in the near future for every protein family at least one member with a known structure will be available, the importance and applicability of homology modelling is steadily increasing. We demonstrate the principles of homology modelling on hand of the Glutamic Acid Decarboxylase (GAD 65) structure prediction, which is a typical autoantigen involved in Diabetes Mellitus Type 1.

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“…These data should be compared and contrasted not only with the mechanisms of autoimmunity, but also specificities of autoantibodies and their genetic basis in other diseases [22][23][24][25][26][27][28][29][30][31][32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Three Anti-CCP Antibody Tests and Rheumatoid Factor in RA and Control Patients

Correia¹,

Carvalho²,

Fortuna

et al. 2007

Clinic Rev Allerg Immunol

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Sera from 86 RA patients and 90 control patients with other diseases, such as infections, connective tissue diseases, or other rheumatic diseases, were analysed using the four different methods. Specificity (84.4%) and PPV (80.3%) were lowest for RF IgM and highest for EliA CCP (specificity 97.8%, PPV 96.7%), the other values being close to those of EliA CCP. Sensitivity was highest for Quanta Lite CCP (73.3%). Efficiency was highest for Quanta Lite CCP (84.1%) at the lower cut off followed by EliA CCP at both cut offs (83.0%). The discrimination between RA sera (mean value 407.67 U/ml) and control sera (4.54 U/ml) and the relative risk (23.34) were best for EliA CCP. The results of this study reveal that there was no advantage of the anti-CCP antibodies assay applying a new antigen mixture (Quanta Lite CCP 3 ELISA) compared to two second generation anti-CCP antibodies assays (Quanta Lite CCP ELISA and EliA CCP).

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Characterisation of an autoreactive conformational epitope on GAD65 recognised by the human monoclonal antibody b78 using a combination of phage display, in vitro mutagenesis and molecular modelling

Cited by 11 publications

References 33 publications

Unravelling autoimmune pathogenesis by screening random peptide libraries with human sera

Unravelling autoimmune pathogenesis by screening random peptide libraries with human sera

Homology Modelling: Eine Übersicht über die Methode am Beispiel der Strukturbestimmung vom Diabetes Antigen GAD 65

Comparison of Three Anti-CCP Antibody Tests and Rheumatoid Factor in RA and Control Patients

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