Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine

Abstract

A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database. Phylogenetic analysis indicated that vB_BbrM_PHB04 is a new member of the family Myoviridae. In addition, polymerase chain reaction-based detection of phage genes in phage-resistant B. bronchiseptica variants revealed no evidence of lysogenic activity of phage vB_BbrM_PHB04.

Specific Integration of Temperate Phage Decreases the Pathogenicity of Host Bacteria

“…Phage isolation and purification were carried out using a doublelayer agar method as described previously (Chen et al, 2019a). Briefly, sewage samples (20 ml each) were collected in Jingzhou, China, and sterilized by filtration through a 0.22-µm membrane.…”

“…To identify strains lysogenized by PHB09, 100 µl of exponentialphase B. bronchiseptica Bb01 culture (∼10 8 CFU/ml) were incubated with 100 µl of purified phage PHB09 (10 9 PFU/ml) as previously described (CLSI, 2018;Chen et al, 2019a). Six milliliters soft TSA (0.75% agar) containing 10% fetal bovine serum was then added to the mixture and then poured onto a prepared TSA plate.…”

“…Phage therapy could be an alternative strategy against drug-resistant B. bronchiseptica infection. In fact, several B. bronchiseptica phages have been isolated (Liu et al, 2004;Petrovic et al, 2017;Chen et al, 2019a), and some of those were tested against B. bronchiseptica infection in our previous study (Chen et al, 2019a).…”

“…We have been working on isolation and characterization of B. bronchiseptica phages since we reported the use of phages to treat B. bronchiseptica infection (Chen et al, 2019a). An interesting temperate phage, PHB09, which has a specific integration site in the host genome, was found from our B. bronchiseptica phage collections.…”

Specific bacteriophage of Bordetella bronchiseptica regulates B. bronchiseptica-induced microRNA expression profiles to decrease inflammation in swine nasal turbinate cells

“…Currently, it is a well-recognized etiological agent in many domestic and wild animal diseases including tracheobronchitis, conjunctivitis, rhinitis, and pneumonia (Garbal et al 2016;Petrovic et al 2017). In particular, it is one of the primary causative agents of atrophic rhinitis and bronchopneumonia in swine (Moore and Kern 2001;Prüller et al 2015;Chen et al 2019). The characteristic lesion of atrophic rhinitis is atrophy of the nasal septum and turbinate, leading to facial deformation (Petrovic et al 2017;De Jong and Nielsen 1990;Brockmeier et al 2002).…”