Abstract:Objectives The aim of this study was to characterise the new hydrophilic fissure sealant, UltraSeal XT® hydro™ (Ultradent Products, USA), and to investigate its in vitro resistance to microleakage after placement on conventionally acid etched and sequentially lased and acid etched molars.Material and Methods The sealant was characterised by Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and Vickers indentation test. Occlusal surfaces… Show more
“…4, 5, respectively), although the filler remained homogeneously distributed in the Group VI specimens. The increased concentration of the filler at the enamel surface is attributed to the enhanced surface roughness of the lased enamel [4, 5], which appears to be mitigated by the lubricating effect of saliva.Fig. 4Back-scattered SEM image of UltraSeal XT ® hydro™ in contact with Group IV air-dried etched and lased enamel; and corresponding EDX maps of carbon, barium, silicon and aluminiumFig.…”
Section: Resultsmentioning
confidence: 99%
“…Immediately after sealing, the teeth were placed in distilled water at 37 °C for 24 h and then thermocycled 3000 times between 5 and 55 °C with a transfer time of 10 s and a dwell time of 30 s [ 4 , 5 ]. Subsequent microleakage was assessed via dye penetration.…”
Section: Methodsmentioning
confidence: 99%
“…Moisture contamination compromises the quality of adhesion at the sealant–enamel interface, and is a common challenge encountered in paediatric dentistry where patient compliance is low [ 3 ]. To address this problem, hydrophilic sealants have appeared on the market during the past decade which are specifically designed to be placed on moist enamel [ 3 , 4 ].…”
For a given sealant, successful pit and fissure sealing is principally governed by the enamel conditioning technique and the presence of moisture contamination. A new generation of hydrophilic resin sealants is reported to tolerate moisture. This study investigates the impact of Er:YAG laser pre-conditioning and moisture contamination on the microleakage of a recent hydrophilic sealant. Occlusal surfaces of extracted human molars were either acid etched (n = 30), or successively lased and acid etched (n = 30). Ten teeth from each group were either air-dried, water-contaminated, or saliva-contaminated prior to sealing with UltraSeal XT® hydro™. Samples were inspected for penetration of fuchsin dye following 3000 thermocycles between 5 and 50 °C, and the enamel–sealant interfaces were observed by scanning electron microscopy (SEM). Significant differences in microleakage were evaluated using the Mann–Whitney U test with Bonferroni adjustment (p = 0.05). Laser pre-conditioning significantly reduced dye penetration irrespective of whether the enamel surface was moist or dry. Microleakage of water-contaminated acid etched teeth was significantly greater than that of their air-dried or saliva-contaminated counterparts. SEM analysis demonstrated good adaptation in all groups with the exception of water-contaminated acid etched teeth which exhibited relatively wide gaps. In conclusion, this hydrophilic sealant tolerates the presence of saliva, although water was found to impair its sealing ability. Laser pre-conditioning significantly decreases microleakage in all cases.
“…4, 5, respectively), although the filler remained homogeneously distributed in the Group VI specimens. The increased concentration of the filler at the enamel surface is attributed to the enhanced surface roughness of the lased enamel [4, 5], which appears to be mitigated by the lubricating effect of saliva.Fig. 4Back-scattered SEM image of UltraSeal XT ® hydro™ in contact with Group IV air-dried etched and lased enamel; and corresponding EDX maps of carbon, barium, silicon and aluminiumFig.…”
Section: Resultsmentioning
confidence: 99%
“…Immediately after sealing, the teeth were placed in distilled water at 37 °C for 24 h and then thermocycled 3000 times between 5 and 55 °C with a transfer time of 10 s and a dwell time of 30 s [ 4 , 5 ]. Subsequent microleakage was assessed via dye penetration.…”
Section: Methodsmentioning
confidence: 99%
“…Moisture contamination compromises the quality of adhesion at the sealant–enamel interface, and is a common challenge encountered in paediatric dentistry where patient compliance is low [ 3 ]. To address this problem, hydrophilic sealants have appeared on the market during the past decade which are specifically designed to be placed on moist enamel [ 3 , 4 ].…”
For a given sealant, successful pit and fissure sealing is principally governed by the enamel conditioning technique and the presence of moisture contamination. A new generation of hydrophilic resin sealants is reported to tolerate moisture. This study investigates the impact of Er:YAG laser pre-conditioning and moisture contamination on the microleakage of a recent hydrophilic sealant. Occlusal surfaces of extracted human molars were either acid etched (n = 30), or successively lased and acid etched (n = 30). Ten teeth from each group were either air-dried, water-contaminated, or saliva-contaminated prior to sealing with UltraSeal XT® hydro™. Samples were inspected for penetration of fuchsin dye following 3000 thermocycles between 5 and 50 °C, and the enamel–sealant interfaces were observed by scanning electron microscopy (SEM). Significant differences in microleakage were evaluated using the Mann–Whitney U test with Bonferroni adjustment (p = 0.05). Laser pre-conditioning significantly reduced dye penetration irrespective of whether the enamel surface was moist or dry. Microleakage of water-contaminated acid etched teeth was significantly greater than that of their air-dried or saliva-contaminated counterparts. SEM analysis demonstrated good adaptation in all groups with the exception of water-contaminated acid etched teeth which exhibited relatively wide gaps. In conclusion, this hydrophilic sealant tolerates the presence of saliva, although water was found to impair its sealing ability. Laser pre-conditioning significantly decreases microleakage in all cases.
“…11 To overcome this issue, the incorporation of fillers in resin sealants have been used to improve the acid-and caries-resistance of dental tissue. Sodium monofluorophosphate, 12 nylon-6 and chitosan, 13 fluoroboroaluminosilicate glass, 14 and Bioglass 45S5 15,16 were already tested. In addition, enamel pre-treatments, such as 45S5 bioactive glass airabrasion, done before sealant application, have been evaluated, improving enamel etchability and reducing microleakage.…”
Section: Methodology Formulation Of Experimental Resin Sealantsmentioning
confidence: 99%
“…In addition, enamel pre-treatments, such as 45S5 bioactive glass airabrasion, done before sealant application, have been evaluated, improving enamel etchability and reducing microleakage. 16 Nevertheless, the incorporation of inorganic fillers may negatively affect the rheological properties of resins, 17 compromising polymer chain mobility and sealing properties, 12 and decreasing the degree of conversion. 18 Polyhexamethylene guanidine hydrochloride (PHMGH) is an organic compound from guanidine family with cationic charge 19 and broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria 19,20,21 and fungi.…”
Section: Methodology Formulation Of Experimental Resin Sealantsmentioning
The aim of this study was to evaluate the influence of polyhexamethylene guanidine hydrochloride (PHMGH) in the physicochemical properties and antibacterial activity of an experimental resin sealant. An experimental resin sealant was formulated with 60 wt.% of bisphenol A glycol dimethacrylate and 40 wt.% of triethylene glycol dimethacrylate with a photoinitiator/co-initiator system. PHMGH was added at 0.5 (G 0.5%), 1 (G 1%), and 2 (G 2%) wt.% and one group remained without PHMGH, used as control (G CTRL). The resin sealants were analyzed for degree of conversion (DC), Knoop hardness (KHN), and softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle (θ) with water or α-bromonaphthalene, surface free energy (SFE), and antibacterial activity against Streptococcus mutans for biofilm formation and planktonic bacteria. There was no significant difference for DC (p > 0.05). The initial Knoop hardness ranged from 17.30 (±0.50) to 19.50 (± 0.45), with lower value for G CTRL (p < 0.05). All groups presented lower KHN after immersion in solvent (p < 0.05). The ΔKHN ranged from 47.22 (± 4.30) to 57.22 (± 5.42)%, without significant difference (p > 0.05). The UTS ranged from 54.72 (± 11.05) MPa to 60.46 (± 6.50) MPa, with lower value for G 2% (p < 0.05). PHMGH groups presented no significant difference compared to G CTRL in θ (p > 0.05). G 2% showed no difference in SFE compared to G CTRL (p > 0.05). The groups with PHMGH presented antibacterial activity against biofilm and planktonic bacteria, with higher antibacterial activity for higher PHMGH incorporation (p < 0.05). PHMGH provided antibacterial activity for all resin sealant groups and the addition up to 1 wt.% showed reliable physico-chemical properties, maintaining the caries-protective effect of the resin sealant over time.
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