Character and laxative activity of polysaccharides isolated from Dendrobium officinale

Luo, Dandan; Qu, Cao; Lin, G. David; Zhang, Zhenbiao; Xie, Jianhui; Chen, Hanbin; Liang, Jia-Li; Li, Cailan; Wang, Hongfeng; Su, Zi‐Ren

doi:10.1016/j.jff.2017.04.024

Cited by 41 publications

(21 citation statements)

References 53 publications

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“…Polysaccharides isolated from D. officinale (DOP) were prepared as previously described [ 23 , 24 ]. First, D. officinale (100 g) was crushed and extracted thrice with distilled water (1 : 20, wt/wt) for 2 h. The combined extracts were filtered to remove impurities.…”

Section: Methodsmentioning

confidence: 99%

Hepatoprotective Effect of Polysaccharides Isolated from Dendrobium officinale against Acetaminophen‐Induced Liver Injury in Mice via Regulation of the Nrf2‐Keap1 Signaling Pathway

Lin

Luo

Liu

et al. 2018

Oxidative Medicine and Cellular Longevity

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The effect of polysaccharides isolated from Dendrobium officinale (DOP) on acetaminophen- (APAP-) induced hepatotoxicity and the underlying mechanisms involved are investigated. Male Institute of Cancer Research (ICR) mice were randomly assigned to six groups: (1) control, (2) vehicle (APAP, 230 mg/kg), (3) N-acetylcysteine (100 mg/kg), (4) 50 mg/kg DOP, (5) 100 mg/kg DOP, and (6) 200 mg/kg DOP. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the serum and glutathione (GSH), malondialdehyde (MDA), catalase (CAT), total antioxidant capacity (T-AOC), myeloperoxidase (MPO), and reactive oxygen species (ROS) levels in the liver were determined after the death of the mice. The histological examination of the liver was also performed. The effect of DOP on the Kelch-like ECH-associated protein 1- (Keap1-) nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway was evaluated using Western blot analysis and real-time polymerase chain reaction (PCR). The results showed that DOP treatment significantly alleviated the hepatic injury. The decrease in ALT and AST levels in the serum and ROS, MDA, and MPO contents in the liver, as well as the increases in GSH, CAT, and T-AOC in the liver, were observed after DOP treatment. DOP treatment significantly induced the dissociation of Nrf2 from the Nrf2−Keap1 complex and promoted the Nrf2 nuclear translocation. Subsequently, DOP-mediated Nrf2 activation triggered the transcription and expressions of the glutamate–cysteine ligase catalytic (GCLC) subunit, glutamate–cysteine ligase regulatory subunit (GCLM), heme oxygenase-1 (HO-1), and NAD(P)H dehydrogenase quinone 1 (NQO1) in APAP-treated mice. The present study revealed that DOP treatment exerted potentially hepatoprotective effects against APAP-induced liver injury. Further investigation about mechanisms indicated that DOP exerted the hepatoprotective effect by suppressing the oxidative stress and activating the Nrf2−Keap1 signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Hepatoprotective Effect of Polysaccharides Isolated from Dendrobium officinale against Acetaminophen‐Induced Liver Injury in Mice via Regulation of the Nrf2‐Keap1 Signaling Pathway

Lin

Luo

Liu

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…13 It has been reported likewise in many studies the use of isolated-polysaccharides for the prevention or treatment of slow-transit induced by various agents. 14…”

Section: Laxative Componentsmentioning

confidence: 99%

Laxative and anti-purgative bioactive compounds in prevention and treatment of functional gastrointestinal disorders, constipation and diarrhea

Rtibi¹

2018

JNHFE

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Normal gastrointestinal functions include transport, digestion, and absorption of nutrients, and removal of waste products. Functional gastrointestinal disorders are characterized by current gastrointestinal disruptions that have opposite pathophysiologic mechanisms such as dismotility and diarrhea. Patients with FGIDs experience a constellation of symptoms consistent with abnormalities in these gastrointestinal functions. 1 Constipation pathogenesis Constipation or intestinal dismotility is an acute or chronic gastrointestinal pathology characterized by infrequent and limited bowel movements, hard and dry stools, inadequate and insufficient bowel evacuation and difficulty in elimination of defecation. 2 Pathophysiology of diarrhea Diarrhea is an increase in the volume of stool or frequency of defecation. The absorption/secretion process of water and electrolytes throughout the gastrointestinal tract is a finely balanced, dynamic process and, imbalance phenomen caused either by decreased absorption or increased secretion, diarrhoea results. 3 A recent study showed that sulphated polysaccharide isolated from the Seaweed Gracilaria caudata more effectively reduced diarrhoea

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“…At present, Dendrobium is widely used as an edible medicinal plant in China. Its most common uses are for cooking in soup, porridge, and dishes as a functional food recorded by the China Food and Drug Administration [1,2]. It has the functions of benefiting the stomach, nourishing the yin, and clearing away heat, and has been used for the treatment of weakness after febrile diseases, thirst, asthenia syndrome, and other diseases [3].…”

Section: Introductionmentioning

confidence: 99%

HPLC coupled with electrospray ionization multistage MS/MS and TLC analysis of flavones‐C‐glycosides and bibenzyl of Dendrobium hercoglossum

Zhou

Huang

et al. 2020

J of Separation Science

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Dendrobium hercoglossum Rchb. f. (D. hercoglossum), as one of the origins of medicinal Dendrobium, has been widely used as a health food and nutrient source promoting fluid production. Due to a lack of quality control, it is often counterfeited or mixed with other Dendrobium. In this study, a highperformance liquid chromatography characteristic chromatogram method is established for the quality evaluation of D. hercoglossum. Based on the highperformance liquid chromatography characteristic chromatogram, D. hercoglossum is divided into two classes, each with different flavone peaks. These flavone peaks were identified using high-performance liquid chromatography coupled with electrospray ionization multistage tandem mass spectrometry. Among them, the acylated (3-hydroxy-3-methylglutaryl, p-coumaroyl, feruloyl, or sinapoyl) flavones-C-glycosides are first found in D. hercoglossum in this study. In addition, one unique band was found in D. hercoglossum by thin-layer chromatography, which can be used to distinguish it from other Dendrobium species as a characteristic marker of this plant. Combining the high-performance liquid chromatography characteristic chromatogram and high-performance liquid chromatography coupled with electrospray ionization multistage tandem mass spectrometry, the unique band was identified as 4,5-dihydroxy-3,3′dimethoxybibenzyl. These analysis methods can be applied for the quality control and identification of D. hercoglossum as well as providing reference for the identification of similar constituents in other Dendrobium species. K E Y W O R D S flavonoids, high-performance liquid chromatography, mass spectrometry, thin-layer chromatography, traditional Chinese medicine Article Related Abbreviations: ESI-MS n , ESI multistage MS/MS; HCA, hierarchical cluster analysis; OPLS-DA, orthogonal partial least square discriminant analysis; PCA, principal analysis analysis; TCM, traditional Chinese medicine; VIP, variable importance plot 1 INTRODUCTION Dendrobium, also called "shihu" in China, is the second largest genus in Orchidaceae, whose medicinal history was originally recorded in "Shen Nong's Materia Medica." At present, Dendrobium is widely used as an edible

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Character and laxative activity of polysaccharides isolated from Dendrobium officinale

Cited by 41 publications

References 53 publications

Hepatoprotective Effect of Polysaccharides Isolated from Dendrobium officinale against Acetaminophen‐Induced Liver Injury in Mice via Regulation of the Nrf2‐Keap1 Signaling Pathway

Hepatoprotective Effect of Polysaccharides Isolated from Dendrobium officinale against Acetaminophen‐Induced Liver Injury in Mice via Regulation of the Nrf2‐Keap1 Signaling Pathway

Laxative and anti-purgative bioactive compounds in prevention and treatment of functional gastrointestinal disorders, constipation and diarrhea

HPLC coupled with electrospray ionization multistage MS/MS and TLC analysis of flavones‐C‐glycosides and bibenzyl of Dendrobium hercoglossum

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