CHAPTER 7. Engineering Stem Cell Niche and Stem Cell–Material Interactions

Lee, B. H.; Tijore, Ajay; Lam, Chee Ren Ivan; Chen, H.; Kumar, K. M.; Tan, Lingping

doi:10.1039/9781782626756-00163

Cited by 2 publications

(2 citation statements)

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“…Both hydrogel material properties and topography play key role in such stem cell behavior. However, the topography has been found to be considerably influential in determining cytoskeletal organization, cell morphology and aligned orientation [20,22,25,62]. Indeed, Yim et al found that between material properties and surface topography, the latter was found to have the greatest effect on cell cytoskeletal arrangement [62].…”

Section: Bioprinting the Hydrogel Microchannels To Align The Cellsmentioning

confidence: 99%

“…In particular, stem cells lineage commitment can be modulated by such surface guidance [20][21][22]. Aligned fibers, grooves, and ridges have been used to orientate cells and stimulate the differentiation of stem cells towards adipogenic, osteogenic, neurogenic, myogenic and cardiomyogenic phenotype [23][24][25][26]. For example, wide channels of shallow nanoscale depth and micron width on a rigid polymethylcrylate surface stimulated osteogenesis in human mesenchymal stem cell (hMSCs) [26], whereas hMSCs cultured on 350 nm scale grooves on polydimethylsiloxane expressed neuronogenesis associated markers [27].…”

Section: Introductionmentioning

confidence: 99%

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Contact guidance for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel

et al. 2018

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Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior. Furthermore, the aligned stem cells showed myocardial lineage commitment, as detected using mature cardiac markers. The fluorescence-activated cell sorting analysis also confirmed a significant increase in the commitment towards myocardial tissue lineage. Moreover, seeded CMs were found to be more aligned and demonstrated synchronized beating on microchanneled hydrogel as compared to the unpatterned hydrogel. Overall, our study proved that microchanneled hydrogel scaffold produced by 3D bioprinting induces myocardial differentiation of stem cells as well as supports CMs growth and contractility. Applications of this approach may be beneficial for generating in vitro cardiac model systems to physiological and cardiotoxicity studies as well as in vivo generating custom designed cell impregnated constructs for tissue engineering and regenerative medicine applications.

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