Changes over time in pulmonary inflammatory response in rat lungs after intratracheal instillation of nickel oxide nanoparticles

Abstract

Objective Nickel oxide nanoparticles (NiONPs) are representative metal oxide NPs and are categorized as an insoluble nickel compound. Our previous studies suggested that NiONPs have more pulmonary toxicity than micron‐sized NiO because they may dissolve slowly and produce many more Ni ions. We confirmed the hypothesis that the slow dissolution of NiONPs induces a change in inflammatory response over time. Method We reanalyzed our previous data on intratracheally instilled NiONP to rats and focused on Ni retention in the lungs and the lung weight ratio for each rat to the mean of control rat lungs. We also measured the solubility of NiONPs and micron‐sized NiO samples by means of an artificial lysosomal fluid (ALF, pH 4.5). Results The in vivo test of instilled NiONPs resulted in the biomarkers reaching their peak values at 1 week or 1 month, and not at 3 days, after instillation. We found that as the NiO mass in the lung increased, the lung weight ratios tended to increase. The relationships shifted to more toxic at 3 days to 1 month ( P < .01). Compared to the dissolution of NiONPs in the ALF that took roughly 1 week, the dissolution of NiONPs in vivo was take about 1 month or more. Conclusion When intratracheally instilled NiONPs dissolve slowly in the phagolysosomes of alveolar macrophages (AM), the resulting Ni ions cause the AM to transform into foamy cells at 1 month, and the inflammatory response persists even at 3 months after instillation.

LncRNA MEG3amelioratesNiOnanoparticles‐induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

“…Additionally, all the changes remained consistent in BALF, lung tissue and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44 …”

“…*p < .05, versus the control group, and # p < .05, versus the 100 μg/ml NiO NPs group and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44.…”

LncRNA MEG3amelioratesNiOnanoparticles‐induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

“…Additionally, all the changes remained consistent in BALF, lung tissue and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44 …”

“…*p < .05, versus the control group, and # p < .05, versus the 100 μg/ml NiO NPs group and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44.…”

Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

“…The electron microscopy study showed that two preparations of NPs, i.e., NP1 and NP2, were largely different in their size distribution, so the quantity of the particles with a diameter less than 50 nm was 2.3 times more in NP1 than in NP2. Considering the available literature data on the significant size effect on Ni-containing particles’ toxicity in vivo [17], it was of interest to establish possible differences in the toxicological characteristics of NP1 and NP2 between themselves, and in comparison to the salt form of Ni.…”

The differential toxicity of three different oxidized nickel compound nanoparticles and the effects of particle surface ligands in mouse alveolar macrophages

“…There are growing concerns that the increased use of ENM, such as Ni-X NP, will add to the burden of lung and systemic diseases in humans exposed to inhalable particles in environmental and occupational settings ( Powers et al 2006 ; Braakhuis et al 2014 ). Inhalation of nano-sized particles can trigger respiratory inflammation, primarily through interactions with alveolar macrophages (AMs) in the lungs ( Hamilton et al 2009 ; Nishi et al 2020 ; Shimizu et al 2023 ; Solorio-Rodriguez et al 2023 ). AMs are the primary lung innate immune cells and take up inhaled particles as part of the first line of defense ( Geiser and Kreyling 2010 ; You et al 2020 ; Hetzel et al 2021 ).…”

LncRNA MEG3amelioratesNiOnanoparticles‐induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

“…Additionally, all the changes remained consistent in BALF, lung tissue and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44 …”

“…*p < .05, versus the control group, and # p < .05, versus the 100 μg/ml NiO NPs group and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44.…”

Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

“…The electron microscopy study showed that two preparations of NPs, i.e., NP1 and NP2, were largely different in their size distribution, so the quantity of the particles with a diameter less than 50 nm was 2.3 times more in NP1 than in NP2. Considering the available literature data on the significant size effect on Ni-containing particles’ toxicity in vivo [17], it was of interest to establish possible differences in the toxicological characteristics of NP1 and NP2 between themselves, and in comparison to the salt form of Ni.…”

The differential toxicity of three different oxidized nickel compound nanoparticles and the effects of particle surface ligands in mouse alveolar macrophages

“…There are growing concerns that the increased use of ENM, such as Ni-X NP, will add to the burden of lung and systemic diseases in humans exposed to inhalable particles in environmental and occupational settings ( Powers et al 2006 ; Braakhuis et al 2014 ). Inhalation of nano-sized particles can trigger respiratory inflammation, primarily through interactions with alveolar macrophages (AMs) in the lungs ( Hamilton et al 2009 ; Nishi et al 2020 ; Shimizu et al 2023 ; Solorio-Rodriguez et al 2023 ). AMs are the primary lung innate immune cells and take up inhaled particles as part of the first line of defense ( Geiser and Kreyling 2010 ; You et al 2020 ; Hetzel et al 2021 ).…”

LncRNA MEG3amelioratesNiOnanoparticles‐induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

“…Additionally, all the changes remained consistent in BALF, lung tissue and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44 …”

“…*p < .05, versus the control group, and # p < .05, versus the 100 μg/ml NiO NPs group and A549 cells. Our results proved that NiO NPs induced lung inflammatory response which was mostly in line with existing research 43,44.…”

Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

“…The electron microscopy study showed that two preparations of NPs, i.e., NP1 and NP2, were largely different in their size distribution, so the quantity of the particles with a diameter less than 50 nm was 2.3 times more in NP1 than in NP2. Considering the available literature data on the significant size effect on Ni-containing particles’ toxicity in vivo [17], it was of interest to establish possible differences in the toxicological characteristics of NP1 and NP2 between themselves, and in comparison to the salt form of Ni.…”

The differential toxicity of three different oxidized nickel compound nanoparticles and the effects of particle surface ligands in mouse alveolar macrophages

“…There are growing concerns that the increased use of ENM, such as Ni-X NP, will add to the burden of lung and systemic diseases in humans exposed to inhalable particles in environmental and occupational settings ( Powers et al 2006 ; Braakhuis et al 2014 ). Inhalation of nano-sized particles can trigger respiratory inflammation, primarily through interactions with alveolar macrophages (AMs) in the lungs ( Hamilton et al 2009 ; Nishi et al 2020 ; Shimizu et al 2023 ; Solorio-Rodriguez et al 2023 ). AMs are the primary lung innate immune cells and take up inhaled particles as part of the first line of defense ( Geiser and Kreyling 2010 ; You et al 2020 ; Hetzel et al 2021 ).…”