“…To date, the only studies of tRNAs and their modification profile during development were conducted in the amoeba Dictyostelium discoideum , which develops from single cell to a multicellular fruiting body in response to starvation (70), and in Drosophila (71), whilst no study addressed so far tRNA expression and modification during development in vertebrates. Here we profiled eleven different tRNA modifications, namely: I, m 1 I, m 1 G, m 1 A, m 2 2 G, m 3 C, m 5 C, acp 3 U, ms 2 i 6 A, ms 2 t 6 A, and o 2 yW.…”