Changes of membrane fatty acids and proteins of Shewanella putrefaciens treated with cinnamon oil and gamma irradiation

Lyu, Fei; Gao, Fei; Wei, Qianqian; Liu, Lin

doi:10.1186/s40643-017-0140-1

Cited by 12 publications

(8 citation statements)

References 44 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aureus, Shewanella putrifaciens, and C. albicans treated with different EOs and EO-ACs. ,,, Such pore formation results in the damage of cell membrane and consequent efflux of intracellular contents including K + ions, nucleotides/DNA/RNA, and protein (Figures B–D and ; panel D).…”

Section: Discussionmentioning

confidence: 99%

“…33 The above findings corroborated with previous studies on planktonic forms of Listeria monocytogenes , Pseudomonas fluorescens , S. aureus , Shewanella putrifaciens , and C. albicans treated with different EOs and EO-ACs. 23,35,37,38 Such pore formation results in the damage of cell membrane and consequent efflux of intracellular contents including K + ions, nucleotides/DNA/RNA, and protein (Figures 6B–D and 9; panel D).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Delineating the Biofilm Inhibition Mechanisms of Phenolic and Aldehydic Terpenes against Cryptococcus neoformans

et al. 2019

View full text Add to dashboard Cite

The recalcitrant biofilm formed by fungus Cryptococcus neoformans is a life-threatening pathogenic condition responsible for further intensifying cryptococcosis. Considering the enhanced biofilm resistance and toxicity of synthetic antifungal drugs, the search for efficient, nontoxic, and cost-effective natural therapeutics has received a major boost. Phenolic (thymol and carvacrol) and aldehydic (citral) terpenes are natural and safe alternatives capable of efficient microbial biofilm inhibition. However, the biofilm inhibition mechanism of these terpenes still remains unclear. In this study, we adopted an integrative biophysical and biochemical approach to elucidate the hierarchy of their action against C. neoformans biofilm cells. The microscopic analysis revealed disruption of the biofilm cell surface with elevation in surface roughness and reduction in cell height. Although all terpenes acted through ergosterol biosynthesis inhibition, the phenolic terpenes also selectively interacted via ergosterol binding. Further, the alterations in the fatty acid profile in response to terpenes attenuated the cell membrane fluidity with enhanced permeability, resulting in pore formation and efflux of the K+/intracellular content. Additionally, mitochondrial depolarization caused higher levels of reactive oxygen species, which led to increased lipid peroxidation and activation of the antioxidant defense system. Indeed, the oxidative stress caused a significant decline in the amount of extracellular polymeric matrix and capsule sugars (mannose, xylose, and glucuronic acid), leading to a reduced capsule size and an overall negative charge on the cell surface. This comprehensive data revealed the mechanistic insights into the mode of action of terpenes on biofilm inhibition, which could be exploited for formulating novel anti-biofilm agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Delineating the Biofilm Inhibition Mechanisms of Phenolic and Aldehydic Terpenes against Cryptococcus neoformans

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The LS has also been used in combination with bioactive molecules in food, such as nisin, reuterin, and Laminaria [40,41]. An antimicrobial effect has also been shown with cinnamon EO acting synergistically with other methods, for example, with gamma irradiation [42] or nisin [43].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of the Lactoperoxidase System and Cinnamon Essential Oil on Total Flora andSalmonellaGrowth Inhibition in Raw Milk

Abbes

Mansouri

Landoulsi

2018

Journal of Food Quality

View full text Add to dashboard Cite

Despite its antibacterial and antipathogenic effects, the heat treatment of milk induces undesirable changes that can be noted in the overall properties of ultrahigh temperature (UHT) milk, such as changes in nutritional and organoleptic properties. Our goal is to find new nonthermal antibacterial technologies for the preservation of raw milk (RM). This study investigates the possible synergistic effect of using a combination of the lactoperoxidase system (LS) and 3 g mL −1 of cinnamon essential oil (cinnamon EO) to inactivate the total flora of milk and Salmonella Hadar (S. Hadar). The LS was activated with 30 mg L −1 sodium percarbonate and 14 mg L −1 of sodium thiocyanate. Using this approach, we obtained a synergistic effect with a complete inhibition of the activity of the total flora of the milk and . Hadar after 12 hours at 25 ∘ C. In addition, the attainment of synergy was defined when the inhibitory effect of the two compounds together was greater than the effect observed by each compound added alone. Moreover, the monitoring of the synergistic effect at 4 ∘ C for 5 days showed complete inhibition of total flora for 3 days and for . Hadar it was up to 5 days. To summarize, the current study clearly identified a new inhibitory combination that may be used in food-based applications.

show abstract

“…The hydrophobicity of EOs and/or their components enables the hydrophobic molecules to preferentially reside in the lipids of the cell membrane and mitochondria, disrupting the structure and rendering cell permeability to be compromised, eventually leading to the leakage of cellular contents [20]. Emerging evidence has revealed that EO antimicrobial activities are attributed to the incorporation of exogenous fatty acids (the EOs) in altering the cell membrane's phospholipid profile [12,38,39]. While phospholipids are the common membrane constituents found in bacteria, plants and mammals, this commonality, however, raises concerns that this particular EO mechanism may be rendered unspecific, as EOs could also exert the same type of toxic effects on any biological membranes.…”

Section: Toxicity Of Eos and Components On The Biological Membranesmentioning

confidence: 99%

Membrane Disruption Properties of Essential Oils—A Double-Edged Sword?

et al. 2021

View full text Add to dashboard Cite

The emerging literature has suggested essential oils (EOs) as new possible weapons to fight antimicrobial resistance due to their inherent antimicrobial properties. However, the potential pharmaceutical use of EOs is confronted by several limitations, including being non-specific in terms of drug targeting, possessing a high cytotoxicity as well as posing a high risk for causing skin irritation. Furthermore, some EOs have been demonstrated to adversely affect the cellular lipid profiles and permeability of the cell membrane, which may result in undesirable outcomes for the cells. Nevertheless, owing to their naturally complex compositions, EOs still hold undiscovered potential to mitigate antimicrobial resistance, as an alternative to existing antibiotics. To address the issue of overuse in antibiotics for crops which have led to the growing threat of antimicrobial resistance globally, EOs have also been proposed as potential biopesticides. Since the perceived advantages of antimicrobial attributes in EOs remain largely unexplored, this review aims to provide a discourse into its current practical usefulness in the agricultural setting. Finally, updated bioengineering techniques with emphasis of the biopesticide potential of EOs as a means to alleviate antimicrobial resistance will be included.

show abstract

Changes of membrane fatty acids and proteins of Shewanella putrefaciens treated with cinnamon oil and gamma irradiation

Cited by 12 publications

References 44 publications

Delineating the Biofilm Inhibition Mechanisms of Phenolic and Aldehydic Terpenes against Cryptococcus neoformans

Delineating the Biofilm Inhibition Mechanisms of Phenolic and Aldehydic Terpenes against Cryptococcus neoformans

Synergistic Effect of the Lactoperoxidase System and Cinnamon Essential Oil on Total Flora andSalmonellaGrowth Inhibition in Raw Milk

Membrane Disruption Properties of Essential Oils—A Double-Edged Sword?

Contact Info

Product

Resources

About