Changes of Isoflavone Contents During Maturation under Different Planting Dates in Black Soybean

Abstract

This study was carried out to investigate the influence of different planting time on the synthesis of isoflavone in black soybean, Three varieties used in this experiment were lpumgeomjeongkong, Cheongjakong and Heugcheongkong which had different ecotypes, repectively. Seeds were sown at different time, May 15th, May 30th and June 15th with planting density of 60x15cm. In order to analyze the content of isoflavone, we collected sample every 5 days from 30 days after flowering to harvest and analyzed them with UPLC. As sowing was delayed, the content of isoflavone increased in all of three varieties. The content of genistein was greater than daidzein and glycitein. Increase of Glycitein was not distinct from 55 days after flowering(DAF) and it was stable against temperature change during the seed developing period. Although the content of genistein in Ilpumgeomjeongkong from 50 to 55 DAF, in Cheongjakong from 40 to 55 DAF and in Heugcheongkong from 60 to 65 DAF was lower than the content of daidzein, it was higher than that of daidzein afterward. In the statistical analysis on the relationship between average temperature and the content of aglycone isoflavone at 5-day intervals from 30 DAF during the grain filling period, genistein in Ilpumgeomjeongkong showed meaningful correlation as y=-15.28x+407.9 (R 2 =0.505 * ), diadzein in Cheongjakong showed meaningful correlation as y=-6.188x-164.5(R 2 =0.454 * ), and genistein showed significantly high correlation as y=-11.59x+297.6 (R 2 =0.545 ** ). Taking all the above results into consideration, it was suggested that the regions suitable for high content of isoflavone in black soybean be the northern area of Gyeonggi-do and Gangwon-do, Chungcheongbuk-do and inland area of Gyeongsangbuk-do, where are relatively low average temperature from flowering stage(R 2 ) during the grain filling period.Keywords : planting time, black soybean, isoflavone, maturation et al., 1996), 암세포에 관 여하는 tryosine-specific protein 작용을 저해하여 발암억제 효과가 있고 (Akiyama, et al., 1987), 폐경기전 여성에서는

Effect of different planting times on the quantitative variation of total seed isoflavone content and composition in Korean soybean cultivars (Glycine max (L.) Merr.)

“…These results suggest that diverse interactions of individual soybean cultivars with the ecological environment during the ripening period based on the planting time might influence the synthesis and accumulation of isoflavone. Yi and Yoon (2012) found that the variations in the time and speed of isoflavone accumulation were different based on the soybean cultivars or planting time when monitored until maturation after flowering of black soybean cultivars.…”

“…The same cultivar can have different isoflavone contents, which is influenced by environmental factors. In particular, different levels of isoflavone content have been reported based on the cultivation environments such as planting time and fertilization level, different weather conditions, and the altitude of the cultivation area (Hasanah et al 2015;Lee et al 2003;Yi and Yoon 2012;Yun et al 2006).…”

Effects of the Cultivated Areas on Antioxidant Compounds and Activities of Proso Millet (Panicum miliaceum L.)

“…작물 의 재배환경에 대한 연구로는 재배환경에 따른 보리의 β -glucan 함량에 관한 연구 에서 β-glucan 함량은 재배지역에 따라 큰 차이가 없는 것으로 보고하였으 며, 쌀의 이화학적 특성에 관한 연구 (Kim & Lim, 1987)에 서 품종별 아밀로그램 특성이 차이가 있는 것으로 보고하였 다. 또한 검정콩에 함유된 안토시아닌의 함량을 재배지역 (Yi et al, 1997) 및 고도 (Shin et al, 2009) (Dewanto et al, 2002). 각 추출물 50 μL 에 2% Na2CO3 용액 1 mL를 가한 후 3분간 방치하여 50% (Fig.…”