Changes of gene expression profiles in the cervical spinal cord by acupuncture in an MPTP-intoxicated mouse model: Microarray analysis

Understanding Acupuncture Based on ZHENG Classification from System Perspective

“…In the genomics study on the treatment effect of acupuncturing on GB34 (Yanglingquan) and LR3 (Taichong) with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced Parkinson's disease animal models, Choi et al analyzed the genetic changes in spinal cord [20] and Corpus Striatum [21] before and after acupuncture treatment based on gene chips technology and validate the results by reverse transcription-polymerase chain reaction (RT-PCR). It was proved that beneficial regulation on genes was developed by acupuncture, which showed their treatment effect by protecting nerves and inhibiting degradation of Corpus Striatum, respectively.…”

“…Omics studies on the acupuncture effects on Parkinson's disease [21, 22, 25, 26], rhinitis [24, 27], osteoarthritis [28], spinal injury [29, 30], pain [18, 19, 23, 25], aging [31, 32], ischemia (ischemic stroke) [33, 34], parturition [35] functional dyspepsia [36, 37], and so forth indicated that the mechanism of acupuncture is involved in the regulation of many body systems and is mainly associated with NEI system (Figure 1). The nerve, endocrine, and immune systems are distributed over the body widely.…”

A review of Omics research in acupuncture: The relevance and future prospects for understanding the nature of meridians and acupoints

“…Four of these genes functioned in an acupointindependent manner. The same model and procedures were used for profiling cervical spinal cord gene expression (Choi et al, 2011b). Although up-and down-regulated genes were observed in both acupoint and non-acupoint treated groups, acupuncture at specific points was able to increase the levels of 5 genes downregulated by MPTP toxicity.…”

Systems Biology of Meridians, Acupoints, and Chinese Herbs in Disease

“…Several studies have performed acupuncture treatments on different acupoints in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-(MPTP-) induced Parkinson's models. These models were used to evaluate the effects of acupuncture treatment at the GB34 and LR3 acupoints by analyzing the transcriptional profiles from cervical spinal cord or brain bilateral striatal tissues [49, 50]. Among the genes downregulated by acupuncture treatment, proplatelet basic protein (Ppbp) was functionally annotated with cytokine-cytokine receptor interaction pathways, and cytotoxic T lymphocyte-associated protein 2 alpha (Ctla2a) was associated with a pathway relevant to Parkinson's disease according to KEGG analysis [49].…”

“…These models were used to evaluate the effects of acupuncture treatment at the GB34 and LR3 acupoints by analyzing the transcriptional profiles from cervical spinal cord or brain bilateral striatal tissues [49, 50]. Among the genes downregulated by acupuncture treatment, proplatelet basic protein (Ppbp) was functionally annotated with cytokine-cytokine receptor interaction pathways, and cytotoxic T lymphocyte-associated protein 2 alpha (Ctla2a) was associated with a pathway relevant to Parkinson's disease according to KEGG analysis [49]. Similar results were observed from substantia nigra tissue of MPTP mice following treatment at the GB34 acupoint [51].…”