Changes induced by co-inoculation in nitrogen–carbon metabolism in cowpea under salinity stress

Santos, Alexandra de Andrade; Silveira, Joaquim Albenísio Gomes da; Guilherme, Eliézer de A.; Bonifácio, Aurenívia; Rodrigues, Artenisa Cerqueira; Figueiredo, Márcia do Vale Barreto

doi:10.1016/j.bjm.2018.01.007

Cited by 28 publications

(12 citation statements)

References 30 publications

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“…A rescue effect and significantly enhanced key amino acid contents were observed in soybean plants inoculated with SA1 compared with non-inoculated soybean plants under heat stress (Table 3). Several studies have demonstrated that an increase in amino acid contents could increase the tolerance to abiotic stress by inoculating plant growth-promoting bacteria [79][80][81][82][83]. Heat stress was found to decrease the accumulation of proline; however, proline levels were enhanced in SA1inoculated soybean plants under control and heat stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Thermotolerance effect of plant growth-promoting Bacillus cereus SA1 on soybean during heat stress

et al. 2020

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Background: Incidences of heat stress due to the changing global climate can negatively affect the growth and yield of temperature-sensitive crops such as soybean variety, Pungsannamul. Increased temperatures decrease crop productivity by affecting biochemical, physiological, molecular, and morphological factors either individually or in combination with other abiotic stresses. The application of plant growth-promoting endophytic bacteria (PGPEB) offers an ecofriendly approach for improving agriculture crop production and counteracting the negative effects of heat stress. Results: We isolated, screened and identified thermotolerant B. cereus SA1 as a bacterium that could produce biologically active metabolites, such as gibberellin, indole-3-acetic acid, and organic acids. SA1 inoculation improved the biomass, chlorophyll content, and chlorophyll fluorescence of soybean plants under normal and heat stress conditions for 5 and 10 days. Heat stress increased abscisic acid (ABA) and reduced salicylic acid (SA); however, SA1 inoculation markedly reduced ABA and increased SA. Antioxidant analysis results showed that SA1 increased the ascorbic acid peroxidase, superoxide dismutase, and glutathione contents in soybean plants. In addition, heat stress markedly decreased amino acid contents; however, they were increased with SA1 inoculation. Heat stress for 5 days increased heat shock protein (HSP) expression, and a decrease in GmHSP expression was observed after 10 days; however, SA1 inoculation augmented the heat stress response and increased HSP expression. The stress-responsive GmLAX3 and GmAKT2 were overexpressed in SA1-inoculated plants and may be associated with decreased reactive oxygen species generation, altered auxin and ABA stimuli, and enhanced potassium gradients, which are critical in plants under heat stress. Conclusion: The current findings suggest that B. cereus SA1 could be used as a thermotolerant bacterium for the mitigation of heat stress damage in soybean plants and could be commercialized as a biofertilizer only in case found non-pathogenic.

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Section: Discussionmentioning

confidence: 99%

Thermotolerance effect of plant growth-promoting Bacillus cereus SA1 on soybean during heat stress

et al. 2020

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“…En las leguminosas exportadoras de ureido, el nitrógeno fijado por los rizobios dentro de los nódulos radiculares se asimila en el aminoácido glutamina (Gln) y se metaboliza en los ureidos alantoina y ácido alantóico, cuya importación hacia el xilema se hace a partir de ureido permeazas (UPS1) (Carter & Tegeder, 2016;Santos et al, 2018); luego, se distribuyen en la planta, pero se acumulan en mayor proporción en tallos (Carter & Tegeder, 2016;Thilakarathna & Raizada, 2018), en especial en el estado vegetativo de las leguminosas, aunque durante el estado reproductivo son transferidos de los tallos a las vainas y a las semillas (Yong et al, 2018), por lo que para la toma de muestras se debe tener en cuenta el estado de desarrollo de la planta.…”

Section: Contenido De Ureidosunclassified

“…Esta técnica es una forma indirecta de evaluar la fijación biológica de nitrógeno, debido a que una reducción en el contenido de ureidos indica menor fijación de los compuestos nitrogenados (Santos et al, 2018), de modo que por no ofrecer resultados exactos de la fijación de nitrógeno está en desuso a corto y mediano plazo (Thilakarathna & Raizada, 2018). Las muestras generalmente corresponden a hojas, tallos, vainas y granos.…”

Section: Contenido De Ureidosunclassified

“…El proceso de fijación biológica de nitrógeno es catalizado por el complejo enzimático nitrogenasa que es altamente susceptible a la presencia de oxígeno, de modo que un mecanismo de protección para evitar su inactivación es la expresión de la proteína leghemoglobina que se encuentra dentro de los nódulos y capta el oxígeno que entra a la célula; así, esta hemoproteína (Santos et al, 2018) evita la oxidación de la nitrogenasa y garantiza la fijación eficiente del nitrógeno (Kataria & Baghel, 2016). El procedimiento consiste en tomar entre 1,25 g y 2 g de nódulos radiculares frescos, que se lavan y se mezclan homogéneamente con cinco volúmenes de solución buffer fosfato (5 °C; pH 6,8; 6,8 mol/L); las muestras se centrifugan a 100 rpm por 15 min a 15 °C; posteriormente, se descarta el sedimento y se vuelve a centrifugar a 39.000 rpm por 20 min a 15 °C, cuyo sedimento rojo luego se mide con un espectrofotómetro (Yong et al, 2018).…”

Section: Medición De Leghemoglobinaunclassified

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Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

Fonseca-López

Quila

Balaguera-López

2019

CTA

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La economía del nitrógeno es un tema que viene creciendo a nivel mundial, especialmente en la actividad agrícola cuando por mal manejo de la fertilización ocurren pérdidas por lixiviación que contribuyen a la eutrofización del agua, lo que incrementa la población de algas y, como efecto, reduce la disponibilidad de oxígeno, acelerando el proceso de desnitrificación en el que se produce N2O, conocido por su efecto en el calentamiento global. Igualmente, parte del fertilizante es emitido a la atmósfera por volatización. Esto ha impulsado la creación de técnicas que permiten cuantificar el nitrógeno utilizado por las plantas y el fijado en el suelo a través de microrganismos para hacer más eficiente el empleo del nitrógeno en los sistemas agrícolas. Sin embargo, dentro de las mayores limitantes se encuentra su sensibilidad, especificidad, costo y tecnología que se requiere para aplicarlas. Esto ha conducido a la innovación de procedimientos y a la creación de técnicas que tienen una tasa de error muy bajo. El objetivo de este trabajo fue realizar la descripción de las principales técnicas utilizadas para cuantificar la fijación del nitrógeno con énfasis en los antecedentes, procedimientos, expresiones matemáticas que se usan, y escenarios futuros. La información se describe a partir del análisis de ensayos disponibles en la base de datos Scopus. Este trabajo consolida las técnicas que continúan vigentes para cuantificar el nitrógeno y facilita entender su uso a través del tiempo con modelos de predicción, así como su importancia, ventajas y desventajas.

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“…Chinnaswamy et al (2018) emphasized that the combination of Ensifer medicae and PGPB B. megaterium NMp082 is synergistic and alleviates salt stress in alfalfa. Santos et al (2018) showed that cowpea coinoculated with Bradyrhizobium sp. and PGPB Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

COINOCULATION WITH Bradyrhizobium AND Trichoderma ALLEVIATES THE EFFECTS OF SALT STRESS IN COWPEA

et al. 2019

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The deleterious effects of salt stress can be mitigated by the use of beneficial microorganisms. The aims of this study were to evaluate whether coinoculation with Bradyrhizobium and Trichoderma asperelloides alleviates salt stress in cowpea. The experiment was conducted in a greenhouse using pots filled with sterile soil. Seeds were sown and inoculated with Bradyrhizobium or coinoculated with Bradyrhizobium and T. asperelloides. At 15 days after sowing (DAS), the nitrogen-free nutritive solution was supplemented with 50 or 100 mmol L -1 sodium chloride (NaCl) to induce salinity. Uninoculated plants and irrigated with solution without NaCl were used as absolute control. At 35 DAS, plants were collected and nodules were excised for use in the determinations. The absolute controls did not show root nodules. Salt stress decreased plant biomass and growth, especially in cowpea inoculated with Bradyrhizobium. The stem diameter increased in cowpea coinoculated with Bradyrhizobium and T. asperelloides, mainly in plants subjected to salt stress at 100 mmol L -1 NaCl. Cowpea coinoculated with Bradyrhizobium and T. asperelloides maintained a higher content of free ammonia and organic compounds in its nodules even under salt stress. We concluded that the coinoculation of cowpea with Bradyrhizobium and T. asperelloides induces an increase in the concentration of organic solutes in the root nodules, especially when cowpeas are cultivated under salinity. Therefore, the use of coinoculation with Bradyrhizobium and T. asperelloides alleviates the negative effects of salt stress in cowpea. COINOCULAÇÃO COMBradyrhizobium E Trichoderma ALIVIA OS EFEITOS DO ESTRESSE SALINO EM FEIJÃO-CAUPI RESUMO -Os efeitos deletérios do estresse salino podem ser mitigados pelo uso de microrganismos benéficos. O objetivo foi avaliar se a inoculação com Bradyrhizobium e Trichoderma asperelloides alivia o estresse salino em feijão-caupi. O experimento foi conduzido em casa de vegetação utilizando vasos preenchidos com solo estéril. As sementes foram semeadas e inoculadas com Bradyrhizobium ou coinoculadas com Bradyrhizobium e T. asperelloides. Aos 15 dias após a semeadura (DAS), a solução nutritiva isenta de nitrogênio foi suplementada com 50 ou 100 mmol L -1 de cloreto de sódio (NaCl) para induzir a salinidade. Plantas não inoculadas e irrigadas com solução sem NaCl foram utilizadas como controle absoluto. Aos 35 DAS, as plantas foram coletadas e os nódulos excisados para uso nas determinações. O controle absoluto não apresentou nódulos radiculares. O estresse salino diminuiu a biomassa e o crescimento das plantas, especialmente no feijão-caupi inoculado com Bradyrhizobium. O diâmetro do caule aumentou no feijão-caupi coinoculado com Bradyrhizobium e T. asperelloides, principalmente nas plantas submetidas a estresse salino com 100 mmol L -1 de NaCl. O feijão-caupi coinoculado com Bradyrhizobium e T. asperelloides manteve um conteúdo mais alto de amônia livre e compostos orgânicos em seus nódulos, mesmo sob estresse salino. Nós concluímos que a coinoc...

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Changes induced by co-inoculation in nitrogen–carbon metabolism in cowpea under salinity stress

Cited by 28 publications

References 30 publications

Thermotolerance effect of plant growth-promoting Bacillus cereus SA1 on soybean during heat stress

Thermotolerance effect of plant growth-promoting Bacillus cereus SA1 on soybean during heat stress

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

COINOCULATION WITH Bradyrhizobium AND Trichoderma ALLEVIATES THE EFFECTS OF SALT STRESS IN COWPEA

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