Changes in the motility patterns of spermatozoa from the rabbit epididymis as assessed by computer-aided sperm motion analysis

Pérez-Sánchez, Francisco; Tablado, L.; Yeung, Ching‐Hei; Cooper, Trevor G.; Soler, Carles

doi:10.1002/(sici)1098-2795(199611)45:3<364::aid-mrd14>3.0.co;2-z

Cited by 19 publications

(9 citation statements)

References 15 publications

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“…The age-old method of sperm motility measurement is the microscopic method that essentially measures horizontal motion of the cells and this method gives only a subjective assessment of sperm motility. Subsequently several objective methods have been developed to measure sperm horizontal movement, the CASA being the most widely used one (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). Although CASA gives multiple sperm motility parameters, their correlation with fertility potential of spermatozoa is not well defined (23)(24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

“…But, all the available techniques consider only the ''horizontal'' velocity and there is not a single instrument available for measuring the ''vertical'' velocity of spermatozoa. The sophisticated computer aided semen analyzer (CASA) based on microscopic video photographic method is widely used for estimating sperm horizontal velocity from the horizontal plane of a glass slide or haemocytometer or Makler Chamber (23)(24)(25)(26)(27). Another objective method of sperm motility analysis is the spectrophotometric/turbidimetric method described by Sokoloski et al (28) and simplified and made user-friendly by Majumder and Chakrabarti (29).…”

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confidence: 99%

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A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: A novel concept

et al. 2007

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Background:The presently available cell motility‐analyzers measure primarily the “horizontal” velocity and there is no instrument available for “vertical” velocity measurement. This development was based on the turbidimetric method of sperm motility analysis.Methods:Sperm was layered at the bottom of the cuvette containing buffer solution and exposed to the spectrophotometric light path at different heights to track the vertically moving sperms. The vertical movement was materialized with the development of an electromechanical up–down movement devise for the cuvette accomplished with the help of a cuvette holder‐stepper motor‐computer assembly. The entire system was controlled by the necessary motion control, data acquisition, and data processing softwares developed for cuvette movement and data analysis.Results:Using goat sperm as the model a unique computer‐based spectrophotometric system has been developed for the first time to determine the average “vertical” velocity of motile cells.Conclusions:Undertaking upward movement against gravity is much tougher as compared with horizontal movement. Consequently average vertical velocity is expected to be a much better identifying parameter for assessing semen and other motile cell quality. The novel instrumental system developed by us has thus the potential for immense application in human infertility clinics, animal‐breeding centres, centres for conservation of endangered species, and also for research work on vertical velocity of spermatozoa and other motile cells, such as bacteria, protozoa, etc. © 2007 International Society for Analytical Cytology

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: A novel concept

et al. 2007

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“…Flagellar beating in both Cavia and Galea develops proximally (distal caput) in the totally immotile sperm that leave the testis. Motility likewise develops within the epididymis of hamsters , rats (Yeung et al, 1992), mice (Soler et al, 1994), and marmoset monkeys , whereas sperm are motile upon entering the epididymis of rabbits (Pérez-Sánchez et al, 1996), cynomolgus monkeys and humans (Yeung et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Maturation of Epididymal Spermatozoa in the Nondomesticated Guinea Pigs Cavia aperea and Galea musteloides

Cooper¹,

Weydert

Yeung³

et al. 2000

Journal of Andrology

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The physiological changes occurring in spermatozoa in the male reproductive tract of 2 nondomesticated species of South American guinea pigs with different mating systems were studied. Cavia aperea, the wild ancestor of the domesticated guinea pig, has a polygynous mating system, whereas Galea musteloides exhibits promiscuous mating behavior. The epididymis of both species resembled that of the domesticated guinea pig, with a swathe of tubule convolutions (linking the 2 major parts of the organ) that was of smaller size in Cavia but not Galea. Higher relative epididymal weight was demonstrated in the promiscuous species. During their journey through the epididymis, spermatozoa from Galeadeveloped their potential for motility expression more proximally than did those of Cavia, but motility developed into forward progression in the same region in both species. The maximal velocities exhibited by mature Caviasperm in vitro were greater than those of Galea.Spermatozoa from Cavia were twice the length of those from Galea, they had larger heads, and the acrosomes of single sperm were more sensitive to disruption during morphological preparation. Only in Cavia did agglutination of sperm into rouleaux occur, after the potential for motility had been developed. Migration of the cytoplasmic droplet along the midpiece occurred in the same regions in both species and before agglutination in Cavia. It is suggested that the male's reproductive strategy (polygyny vs promiscuity) dictates the size of the testis and epididymis, whereas the female's reproductive physiology (induced ovulation vs cyclicity) influences the posttesticular development of sperm morphology and motility in the epididymis.

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“…Testicular spermatozoa from marsupial and eutherian mammals show no motility, or only weak vibrations of the tail with no progressive motility (Voglmayr et al 1966;Setchell 1970;Tuck et al 1970); this form of motility and the capacity to undergo hyperactivation develop during epididymal transit (OrgebinCrist 1967; Jones et al 1984;Clulow et al 1992;Pérez-Sánchez et al 1996). Testicular spermatozoa from the echidna also do not display progressive motility, and although we found that soluble forms of cAMP or phosphodiestrase inhibitors can stimulate activity, the flagellum beat remains weak and motility is non-progressive (K. Krivanek, B. Nixon and R. C. Jones, unpubl.…”

Section: Epididymal Sperm Maturationmentioning

confidence: 98%

Testicular descent, sperm maturation and capacitation. Lessons from our most distant relatives, the monotremes

Ecroyd

Nixon

Dacheux

et al. 2009

Reprod. Fertil. Dev.

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The present review examines whether monotremes may help to resolve three questions relating to sperm production in mammals: why the testes descend into a scrotum in most mammals, why spermatozoa are infertile when they leave the testes and require a period of maturation in the specific milieu provided by the epididymides, and why ejaculated spermatozoa cannot immediately fertilise an ovum until they undergo capacitation within the female reproductive tract. Comparisons of monotremes with other mammals indicate that there is a need for considerable work on monotremes. It is hypothesised that testicular descent should be related to epididymal differentiation. Spermatozoa and ova from both groups share many of the proteins that are thought to be involved in gamete interaction, and although epididymal sperm maturation is significant it is probably less complex in monotremes than in other mammals. However, the monotreme epididymis is unique in forming spermatozoa into bundles of 100 with greatly enhanced motility compared with individual spermatozoa. Bundle formation involves a highly organised interaction with epididymal proteins, and the bundles persist during incubation in vitro, except in specialised medium, in which spermatozoa separate after 3 h incubation. It is suggested that this represents an early form of capacitation. Abstract. The present review examines whether monotremes may help to resolve three questions relating to sperm production in mammals: why the testes descend into a scrotum in most mammals, why spermatozoa are infertile when they leave the testes and require a period of maturation in the specific milieu provided by the epididymides, and why ejaculated spermatozoa cannot immediately fertilise an ovum until they undergo capacitation within the female reproductive tract. Comparisons of monotremes with other mammals indicate that there is a need for considerable work on monotremes. It is hypothesised that testicular descent should be related to epididymal differentiation. Spermatozoa and ova from both groups share many of the proteins that are thought to be involved in gamete interaction, and although epididymal sperm maturation is significant it is probably less complex in monotremes than in other mammals. However, the monotreme epididymis is unique in forming spermatozoa into bundles of 100 with greatly enhanced motility compared with individual spermatozoa. Bundle formation involves a highly organised interaction with epididymal proteins, and the bundles persist during incubation in vitro, except in specialised medium, in which spermatozoa separate after 2-3 h incubation. It is suggested that this represents an early form of capacitation.

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Changes in the motility patterns of spermatozoa from the rabbit epididymis as assessed by computer-aided sperm motion analysis

Cited by 19 publications

References 15 publications

A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: A novel concept

A computerized spectrophotometric instrumental system to determine the “vertical velocity” of sperm cells: A novel concept

Maturation of Epididymal Spermatozoa in the Nondomesticated Guinea Pigs Cavia aperea and Galea musteloides

Testicular descent, sperm maturation and capacitation. Lessons from our most distant relatives, the monotremes

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