Changes in the Expression of Small Intestine Extracellular Matrix Proteins in Streptozotocin‐induced Diabetic Rats

Sánchez, Sara S.; Genta, Susana B.; Aybar, Manuel J.; Honoré, Stella M.; Villecco, Evelina I.; Riera, Alicia N. Sánchez

doi:10.1006/cbir.2000.0581

Cited by 23 publications

(20 citation statements)

References 27 publications

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“…Increased muscle weight in the small intestine was also noted in a subsequent study (30). Furthermore, in the small intestine, proliferation of epithelial cells and hypertrophy of the mucosa have been observed in conjunction with alterations of the smooth muscle layer in rat duodenum (35). The development of rhythmic contractions in both control and diabetic colon was primarily due to myogenic mechanisms and/or ICC.…”

Section: Discussionmentioning

confidence: 60%

Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus

Forrest¹,

Huizinga²,

Wang³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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Diabetes affects many aspects of gastrointestinal motility, in part due to changes in interstitial cells of Cajal (ICC). The effect of diabetes on the colon, however, is not well characterized, and the aim of the present study was to investigate possible relationships between altered colonic motility as a consequence of streptozotocin-induced diabetes and injury to ICC. Physiological, immunohistochemical, and ultrastructural techniques were employed. The motor pattern of the rat colon was dominated by rhythmic high-amplitude, low-frequency contractions that were primarily myogenic in origin. These rhythmic contractions were induced by stretch associated with increased tension; the amplitude of the superimposed rhythmic contractions increased with increasing applied tension. In diabetic rats, the stretch-induced rhythmic contractile activity remained robust and of similar frequency but was significantly higher in amplitude compared with that in control rats. At 700 mg of applied tension, the force of contraction in circular colonic muscle strips of the diabetic rats was 370% of control values. This robust presence of low-frequency contractions is consistent with the unaffected pacemaker, the ICC associated with Auerbach's plexus, and the increased amplitude correlates with loss of and injury to ICC of the submuscular plexus and intramuscular ICC. Loss of inhibitory nitrergic nerves does not appear to be a factor based on unaltered nNOS immunoreactivity.

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Section: Discussionmentioning

confidence: 60%

Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus

Forrest¹,

Huizinga²,

Wang³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…AGEs are generated by sequential non-enzymatic glycation of protein amino groups and by oxidation reaction [21]. Studies on diabetes and aging show that AGEs cause the cross-linking of collagen molecules responsible for basement membrane thickening and the loss of matrix elasticity [22][23][24]. Sán-chez et al [23] demonstrated the expression of small intestinal extracellular matrix protein changes in STZinduced diabetic rats.…”

Section: Biomechanical Remodelling Of the Diabetic Colonmentioning

confidence: 99%

“…Studies on diabetes and aging show that AGEs cause the cross-linking of collagen molecules responsible for basement membrane thickening and the loss of matrix elasticity [22][23][24]. Sán-chez et al [23] demonstrated the expression of small intestinal extracellular matrix protein changes in STZinduced diabetic rats. Kandemir et al [25] found that colonic subepithelial collagenous thickening occurred in diabetic patients.…”

Section: Biomechanical Remodelling Of the Diabetic Colonmentioning

confidence: 99%

Biomechanical and Histomorphometric Colon Remodelling in STZ-Induced Diabetic Rats

Zhao

Nakaguchi

2008

Dig Dis Sci

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The histomorphologic and passive biomechanical properties were studied in the mid-colon of 16 non-diabetic and 20 streptozotocin (STZ)-induced diabetic rats (50 mg/kg STZ, ip). The diabetic rats were divided into groups living 4 and 8 weeks after the induction of diabetes (n = 10 for each group). The mechanical test was a ramp distension of fluid into the colon in vitro. The colon diameter and length were obtained from digitized images of the segments at pre-selected pressures and at the no-load and zero-stress states. Circumferential and longitudinal stresses and strains were computed from the length, diameter, and pressure data and from the zero-stress state geometry. The blood glucose level increased 3-4-fold in the diabetic rats compared with the controls (P < 0.001). Diabetes generated pronounced increases in the colon weight per length, wall thickness, and wall cross-sectional area (P < 0.001). Histologically, the thickness of all layers was increased during diabetes (P < 0.05), especially the mucosa layer. The opening angle, and absolute values of residual strain increased in the diabetic group (P < 0.05 and P < 0.01, respectively). Furthermore, diabetes increased the circumferential and longitudinal stiffness of the colon wall (P < 0.001). The observed changes in residual strain, opening angle, and stress-strain relation may be contributing factors to colonic dysfunction and abdominal pain in diabetic patients.

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“…17 Specimens fixed in Bouin's solution were dehydrated through a graduated alcohol series and embedded in Paraplast. The sections were cut into 7-µm thick slices, deparaffinized and rinsed with PBS (pH 7.4).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Renal extracellular matrix alterations in lead‐treated rats

Sánchez

Aguilar

Genta

et al. 2001

J of Applied Toxicology

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Although the nephrotoxic effects of lead are well documented, the subcellular mechanisms of its action on the kidney remain unclear. The aim of the present work was to investigate the effects of chronic lead exposure on the expression of laminin-1 and fibronectin in the kidney of lead-treated rats. Western immunoblotting of the kidney extracts revealed that experimental exposure to lead resulted in a marked decrease in the intensity of the bands corresponding to laminin-1 and an increase in the intensity of the band corresponding to fibronectin. Immunohistochemical studies demonstrated a weak labelling to laminin-1 and a strong labelling to fibronectin in all renal basement membranes together with a decrease in their thickness. Other ultrastructural alterations found were a diminution in the amount of endothelial fenestrae, an increased fusion of foot processes in epithelial cells of the glomerulus and the presence of intranuclear inclusion bodies in the proximal tubule cells. Lead intoxication might be responsible for the above alterations in the renal extracellular matrix that could play an important role in the pathogenesis of lead nephropathy.

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Changes in the Expression of Small Intestine Extracellular Matrix Proteins in Streptozotocin‐induced Diabetic Rats

Cited by 23 publications

References 27 publications

Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus

Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus

Biomechanical and Histomorphometric Colon Remodelling in STZ-Induced Diabetic Rats

Renal extracellular matrix alterations in lead‐treated rats

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