Changes in the content and intensity of oxidation of lipid compounds in the kidneys of lambs fed diets with rapeseed and fish oils – effect of antioxidant supplementation

Enrichment of lamb rations with carnosic acid and seleno-compounds affects the content of selected lipids and tocopherols in the pancreas

“…The results summarised in Table 4 show that the experimental diets containing CAc, regardless of the presence of SeYe, stimulated Δ9-desaturation of C16:0 and C18:0 in the pancreas. This was consistent with previous studies showing that CAc, irrespective of the presence of SeYe, increased Δ9-desaturase capacity by stimulating stearoyl-CoA desaturase mRNA expression in kidneys and adipose tissues (Calvo et al, 2017;Krajewska-Bienias et al, 2017;Białek and Czauderna, 2019;Białek et al, 2020Białek et al, , 2021. In contrast, CAc without or with SeYe addition to the experimental diet reduced C18:0 Δ9-desaturation in the liver and brain compared to the control diet (Rozbicka-Wieczorek et al, 2016a,b).…”

“…Thus, the main aim of our study was to analyse the possibility that dietary CAc, with or without 0.35 ppm Se (as SeVI or SeYe), could decrease peroxidative damage of PUFA in the pancreas of lambs fed the diets enriched in RO and FO, containing high proportion of pro-healthy n-3LPUFA. Fortunately, our current and previous studies documented that the addition of 0.35 ppm Se (as SeYe or SeVI) to the diet containing RO, FO and CAc did not cause harmful symptoms (such as diarrhoea or vomiting) and visual changes either in the pancreas or in the liver, spleen, kidney, brain and muscles of lambs (Czauderna et al, 2017;Białek and Czauderna, 2019;Białek et al, 2020Białek et al, , 2021. Indeed, only diets supplemented with inorganic Se-compounds (especially selenite or selenides), at concentrations exceeding 5 mg Se/kg diet exerted hepatotoxic and teratogenic effects in lambs or cows, but also in humans (Navarro-Alarcon and Cabrera-Vique, 2008).…”

Organic and Inorganic Selenium Compounds Affected Lipidomic Profile of Spleen of Lambs Fed with Diets Enriched in Carnosic Acid and Fish Oil

“…Indeed, the level of MDA tended to decrease ( p > 0.05) and the index values of ΣPUFA MDA index and index A SFA in the spleen of lambs receiving the Se Ye CA diet were lower ( p ≤ 0.05) than in the spleen of ewes receiving the Se 6 CA diet. The earlier results [ 37 , 39 ] obtained for the pancreas and kidneys also seem to support the above-mentioned assumptions. Thus, we argued that the higher levels of ROS, RNS, or other radicals in the spleen of the Se Ye CA-treated lambs may cause inflammation of this internal organ [ 20 ].…”

“…Importantly, dietary CA modifies ruminal microbiota, the capacity of isomerization of UFA and BH of UFA, and, hence, the ruminal biosynthesis of VFA (in a dose-dependent manner) and profiles of FA and health-promoting conjugated FA in ruminants’ tissues and internal organs of farm animals [ 28 , 29 , 30 , 31 , 32 , 33 ]. CA (like Se compounds) possesses antioxidative properties and hence protects PUFA, particularly LPUFA, derived from dietary R-O and F-O, from peroxidation damage in ovine tissues [ 28 , 32 , 34 , 35 , 36 , 37 , 38 , 39 ]. Furthermore, our previous studies showed that diets enriched in Se (as Se Ye or Se 6 ), CA, and F-O affected the biosynthesis yield of Se-proteins as well as the profile of lipid compounds in the rumen microbiota [ 26 , 31 , 32 ], blood [ 28 ], muscles [ 35 ], adipose tissues [ 34 , 35 ], and internal organs like the brain [ 36 ], kidneys [ 37 ], heart [ 38 ], and pancreas [ 39 ] of farm animals (like lambs).…”

“…CA (like Se compounds) possesses antioxidative properties and hence protects PUFA, particularly LPUFA, derived from dietary R-O and F-O, from peroxidation damage in ovine tissues [ 28 , 32 , 34 , 35 , 36 , 37 , 38 , 39 ]. Furthermore, our previous studies showed that diets enriched in Se (as Se Ye or Se 6 ), CA, and F-O affected the biosynthesis yield of Se-proteins as well as the profile of lipid compounds in the rumen microbiota [ 26 , 31 , 32 ], blood [ 28 ], muscles [ 35 ], adipose tissues [ 34 , 35 ], and internal organs like the brain [ 36 ], kidneys [ 37 ], heart [ 38 ], and pancreas [ 39 ] of farm animals (like lambs). That is why we also hypothesized that the bioaccumulation of FA, total cholesterol (TCh), tocopherols (Ts) (antioxidants), and the MDA content in the ovine spleen depend upon Se compounds (antioxidants) added to the diet enriched in F-O, R-O, and CA.…”