Changes in serum proteins in dogs with Ehrlichia canis infection

Escribano, Damián; Cihan, Hüseyin; Martı́nez-Subiela, Silvia; Levent, Pinar; Kocatürk, Meriç; Aytuğ, Nilüfer; Cerón, José J.; Tvarijonaviciute, Asta; Yılmaz, Zeki

doi:10.1016/j.micpath.2017.10.024

Cited by 23 publications

(19 citation statements)

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“…Experimentally infected dogs generated TRP36 antibody responses within a short time postinfection 20 . Conversely, plasma protein analyses in dogs with CME have indicated that total protein concentrations increase significantly at the onset of infection 26,27 . These findings suggest the possible involvement of protein profiles in animals seroreactive to different genotypes (USTRP36 and BrTRP36).…”

Section: Discussionmentioning

confidence: 99%

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Taques

Koiyama

Campos

et al. 2020

Veterinary Clinical Pathol

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BackgroundImmunoreactive tandem repeat proteins (TRPs) in amino acid sequences were identified and employed in the serologic diagnosis of canine monocytic ehrlichiosis (CME).ObjectivesThis study evaluated using TRP19 and TRP36 synthetic protein antigens with enzyme‐linked immunosorbent assays (ELISAs) and compared the results with an indirect fluorescent antibody test (IFAT) to diagnose CME in the serum of dogs with suspected CME.MethodsThe sera of 243 dogs that exhibited clinical and hematologic signs suggestive of CME had IFATs performed. An ELISA with synthetic TRP19 and TRP36 antigens from two E canis genotypes (USTRP36 and BrTRP36) that circulate in Brazil were also performed. Canonical correlations and chi‐square tests were evaluated on the results of these tests.ResultsAmong the 243 dogs, 179 (73.6%) were reactive at an IFAT ≥ 40, 167 (68.72%) reacted at the cut‐off ≥160, and 149 (61.31%) reacted at ≥640. The ELISA revealed that 172 (70.8%) dogs reacted to TRP19 peptide, 163 (67.1%) reacted to USTRP36, and 114 (46.9%) reacted to BrTRP36. The canonical correlation analyses showed that the TRP19 ELISA was the most effective diagnostic method. Serum total protein levels showed strong positive correlations with the USTRP36 peptide. Qualitative analyses revealed an association between the TRP19 peptide and the presence of anemia, thrombocytopenia, and hyperproteinemia. The IFAT results at titers of ≥160 and ≥640 were associated with thrombocytopenia.ConclusionsThe results of this study indicated that the TRP19 peptide was an excellent antigen and that the IFAT was diagnostically important in confirming the diagnosis of CME in serum samples.

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Section: Discussionmentioning

confidence: 99%

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Taques

Koiyama

Campos

et al. 2020

Veterinary Clinical Pathol

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“…It has been shown that infection with tick-borne pathogens such as R. conorii , B. henselae , A. platys , A. phagocytophilum and E. canis may induce a decrease in serum concentration of negative acute phase proteins and an increase of positive acute phase proteins [ 19 , 52 – 55 ]. Albumin is a negative acute phase protein whose level tends to decrease in inflammation or infection [ 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?

et al. 2018

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BackgroundThe severity of canine leishmaniosis (CanL) due to Leishmania infantum might be affected by other vector-borne organisms that mimic its clinical signs and clinicopathological abnormalities. The aim of this study was to determine co-infections with other vector-borne pathogens based on serological and molecular techniques in dogs with clinical leishmaniosis living in Spain and to associate them with clinical signs and clinicopathological abnormalities as well as disease severity.MethodsSixty-one dogs with clinical leishmaniosis and 16 apparently healthy dogs were tested for Rickettsia conorii, Ehrlichia canis, Anaplasma phagocytophilum and Bartonella henselae antigens by the immunofluorescence antibody test (IFAT) and for E. canis, Anaplasma spp., Hepatozoon spp., Babesia spp. and filarioid DNA by polymerase chain reaction (PCR).ResultsAmong the dogs examined by IFAT, the seroprevalences were: 69% for R. conorii, 57% for E. canis, 44% for A. phagocytophilum and 37% for B. henselae; while the prevalences found by PCR were: 8% for Ehrlichia/Anaplasma, 3% for Anaplasma platys and 1% for H. canis. No other pathogen DNA was detected. Statistical association was found between dogs with clinical leishmaniosis and seroreactivity to R. conorii antigen (Fisher’s exact test: P = 0.025, OR = 4.1, 95% CI = 1–17) and A. phagocytophilum antigen (Fisher’s exact test: P = 0.002, OR = 14.3, 95% CI = 2–626) and being positive to more than one serological or molecular tests (co-infections) (Mann-Whitney test: U = 243, Z = -2.6, n1 = 14, n2 = 61, P = 0.01) when compared with healthy dogs. Interestingly, a statistical association was found between the presence of R. conorii, E. canis, A. phagocytophilum and B. henselae antibodies in sick dogs and some clinicopathological abnormalities such as albumin and albumin/globulin ratio decrease and increase in serum globulins. Furthermore, seroreactivity with A. phagocytophilum antigens was statistically associated with CanL clinical stages III and IV.ConclusionsThis study demonstrates that dogs with clinical leishmaniosis from Catalonia (Spain) have a higher rate of co-infections with other vector-borne pathogens when compared with healthy controls. Furthermore, positivity to some vector-borne pathogens was associated with more marked clinicopathological abnormalities as well as disease severity with CanL.

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“…Fibrinogen was measured in citrate plasma with a viscosity‐based analytic method (Start Hemostasis analyzer, Diagnostic Stago) 33 . Albumin was measured using heparinized plasma using a colorimetric assay 34 with a commercially available bromocresol green reagent (Albumin OSR 6102 Olympus Life and Material Science Europe GmbH—Irish branch—Lismeehan, O'Callaghan's Mills, Co.). Haptoglobin was determined in heparinized plasma with a colorimetric assay (Tridelta Phase Haptoglobin Assay, Tridelta Ltd.) previously validated for use in dogs 34 and adapted to an automated chemistry analyzer (Olympus 2700 Automatic Chemistry Analyzer, Olympus Europe GmbH).…”

Section: Methodsmentioning

confidence: 99%

“…Albumin was measured using heparinized plasma using a colorimetric assay 34 with a commercially available bromocresol green reagent (Albumin OSR 6102 Olympus Life and Material Science Europe GmbH—Irish branch—Lismeehan, O'Callaghan's Mills, Co.). Haptoglobin was determined in heparinized plasma with a colorimetric assay (Tridelta Phase Haptoglobin Assay, Tridelta Ltd.) previously validated for use in dogs 34 and adapted to an automated chemistry analyzer (Olympus 2700 Automatic Chemistry Analyzer, Olympus Europe GmbH). Ferritin concentration was measured in heparinized plasma with an immunoturbidimetric assay using polyclonal anti‐human ferritin antibodies determined via quantitative assays (Tinaquant Ferritin, Boehringer M) previously validated for use in dogs 35 .…”

Section: Methodsmentioning

confidence: 99%

“…Fibrinogen was measured in citrate plasma with a viscosity-based analytic method (Start Hemostasis analyzer, Diagnostic Stago). 33 Albumin was measured using heparinized plasma using a colorimetric assay 34 with a commercially available bromocresol green reagent (Albumin Total plasma ADPN concentration was measured in heparinized plasma samples using a canine-specific ELISA also previously validated for use in dogs (Canine Adiponectin ELISA, Millipore). 37 Each assay was performed in duplicate as all of the methods used in our study had intra-and inter-assay coefficients of variation below 15%.…”

Section: Sample Analysismentioning

confidence: 99%

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Adiponectin as a sepsis biomarker in dogs: Diagnostic and prognostic value

Torrente

Manzanilla

Bosch

et al. 2020

Veterinary Clinical Pathol

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Background: Adiponectin (ADPN) is an adipocytokine with insulin-sensitizing, vascular-protective, and anti-inflammatory properties for which concentration changes occur in response to inflammation. Little is known about the regulation of ADPN and the impact of this adipocytokine in septic dogs. Objective: We aimed to assess the diagnostic and prognostic value of ADPN vs other traditional acute-phase proteins (APPs), such as albumin (ALB), haptoglobin (HPT), fibrinogen (FBG), ferritin (FRT), and C-reactive protein (CRP) in dogs with naturally acquired sepsis. Methods: This prospective observational study included 20 dogs with sepsis, 27 with low-grade systemic inflammation (LGSI), and 18 clinically healthy dogs as controls. For method analyses, plasma samples were obtained from all dogs on admission and then every 24-48 hours until discharge or death in the septic group. Results: Septic dogs had lower ADPN (2.4 ± 0.46 vs 4.5 ± 0.41mg/L, P < .001) and ALB (17 ± 1 vs 22 ± 0.8g/L, P = .002), and tended to have higher CRP (87 ± 4.8 vs 73 ± 4.1mg/L, P < .079) concentrations than dogs with LGSI on admission. Only ADPN and ALB were able to successfully discriminate animals with LGSI from those presenting with sepsis with areas under the curve (AUCs) for the receiver operating characteristic (ROC) curves of 0.811 and 0.789, respectively. In the septic group, ADPN concentration did not differ between survivors and non-survivors, either on admission or at discharge or death. Conclusions: Although plasma ADPN can be used as a reliable negative APP in dogs with sepsis, further studies are warranted to confirm the usefulness of this biomarker in terms of disease progression and recovery.

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Changes in serum proteins in dogs with Ehrlichia canis infection

Cited by 23 publications

References 22 publications

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?

Adiponectin as a sepsis biomarker in dogs: Diagnostic and prognostic value

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