Changes in protein abundance and activity induced by drought during generative development of winter barley ( Hordeum vulgare L.)

Gołębiowska-Pikania, Gabriela; Kopeć, Przemysław; Surówka, Ewa; Janowiak, F.; Krzewska, Monika; Dubas, Ewa; Nowicka, Anna; Kasprzyk, Joanna; Ostrowska, Agnieszka; Malaga, Sabina; Hura, Tomasz; Żur, Iwona

doi:10.1016/j.jprot.2017.07.016

Cited by 14 publications

(19 citation statements)

References 54 publications

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“…Among various parameters identified in our previous studies (Gołębiowska-Pikania et al 2017a), the most important role in tolerance to freezing and limited water availability seems to be played by effective antioxidative defence (Gołębiowska-Pikania et al 2017b). As the majority of phytohormones are involved in the regulation of antioxidative system activity the level of ABA, SA and BRs was correlated with the activities of antioxidative enzymes-SOD, CAT and nonspecific peroxidases (POX)-as well as with the activity of low molecular weight antioxidants (LMW AOX) measured simultaneously and published earlier by Gołębiowska-Pikania et al (2017a, b).…”

Section: Correlation Analysis Among Stress Tolerance Level Phytohormmentioning

confidence: 96%

Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

et al. 2019

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Ten doubled haploid (DH) lines of winter barley with an increased range of freezing/drought tolerance were used to identify phytohormones involved in plant stress acclimation. Cold hardening and drought stress were applied at the most critical stages of plant development on young seedlings and heading plants, respectively. The level of the phytohormones was significantly higher at heading, more than 5-fold in respect of salicylic acid (SA) and total brassinosteroids (BRs) and 1.7-fold in the case of abscisic acid (ABA). Moreover, the spectrum of detectable BRs increased from one-homocastasterone (HCS)-found in seedlings to four BRs identified in heading plants [HCS, castasterone (CS), teasterone and dolicholide], with the last one detected for the first time in cereal species. To some extent freezing tolerance seems to be determined by native hormonal status as control seedlings of tolerant DH lines contained 1.4-and 2.3-fold lower amount of ABA and HCS and 2.3-fold higher amount of SA in comparison to freezing-sensitive ones. Such dependency was not observed in heading plants as significant variation in CS content was the only detected difference. Under stress treatments, tolerant DH lines accumulated significantly lower (75-81%) amount of ABA, which probably reflected lower stress intensity resulting from another defence strategy. In contrast, stress-induced significant almost 2-fold increase in HCS/CS and 2-3-fold decrease in SA content specific for tolerant DH lines of barley suggest the involvement of these molecules in freezing/drought defence. Detected correlations suggest their interaction with nonspecific peroxidase and low molecular weight antioxidants.

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Section: Correlation Analysis Among Stress Tolerance Level Phytohormmentioning

confidence: 96%

Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

et al. 2019

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“…The six DH lines of winter barley used in this study were selected from DHs produced by androgenesis initiation and they showed increased variation in freezing and drought tolerance in comparison with their parental genotypes [16]. Their thorough examination allowed the identification of several physiological and metabolic parameters associated with stress tolerance acquisition [8][9][10]. In particular, the analysis of proteome profiles provided a basis for the selection of the genes whose expression changes were analyzed in this study [8,9].…”

Section: Discussionmentioning

confidence: 99%

“…Selection of candidate genes for expression studies was based on the results of previous proteomic studies [8,9]. The proteins potentially involved in the response to each of the tested treatments and differentiating between tolerant and susceptible lines were selected and analyzed in regard to their amino acid sequences.…”

Section: Candidate Genes Selectionmentioning

confidence: 99%

“…Six doubled haploid (DH) lines of winter barley examined thoroughly in previous studies with respect to parameters involved in freezing and drought tolerance [8][9][10] were used in the present study. Both of these stress factors impose a water deficit and are considered serious threats to winter barley productivity, especially when they occur at the most critical and sensitive stages, i.e., on young seedlings (freezing) and on plants at the booting stage (drought).…”

Section: Introductionmentioning

confidence: 99%

“…Both of these stress factors impose a water deficit and are considered serious threats to winter barley productivity, especially when they occur at the most critical and sensitive stages, i.e., on young seedlings (freezing) and on plants at the booting stage (drought). The studied DH lines were selected from a population of DHs produced using the anther culture technique from Polish breeding materials and showed increased variation in freezing and drought tolerance levels in comparison with their parental genotypes [8][9][10]. The selected DH lines were examined previously with respect to changes induced by water deficit in the functioning of the photosynthetic apparatus, antioxidative defense and proteome and phytohormone accumulation.…”

Section: Introductionmentioning

confidence: 99%

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Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

Wójcik-Jagła

Rapacz

Dubas

et al. 2020

IJMS

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Plant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha (EF1A), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region (GroEs), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.

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Phenol-Based Protein Extraction Method for Plant Proteomic Studies

Kopeć,

Krzewska,

Płażek

2024

Methods in Molecular Biology

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Changes in protein abundance and activity induced by drought during generative development of winter barley ( Hordeum vulgare L.)

Cited by 14 publications

References 54 publications

Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

Phenol-Based Protein Extraction Method for Plant Proteomic Studies

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