Changes in Na+/K+-ATPase activity, unsaturated fatty acids and metallothioneins in gills of the shore crab Carcinus aestuarii after dilute seawater acclimation

Lucu, Čedomil; Pavičić, Jasenka; Ivanković, Dušica; Pavičić-Hamer, Dijana; Najdek, Mirjana

doi:10.1016/j.cbpa.2008.01.026

Cited by 38 publications

(26 citation statements)

References 56 publications

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“…In our study, we did indeed see that high ROS production in posterior gills was accompanied by increased antioxidant activity. A study by Lucu et al (2008) also showed that long-term acclimation of Mediterranean green crabs to 10 ppt resulted in a significant increase of metallothionein content in posterior gills, which may have a further antioxidant role. These reports highlight that while posterior gill tissues support the osmoregulation procedure, they are clearly capable of putting in place the necessary mechanisms to minimize the OS derived from enhanced ROS formation.…”

Section: Discussionmentioning

confidence: 94%

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Rivera‐Ingraham

Barri

Boël

et al. 2015

Journal of Experimental Biology

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Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H 2 DFFDA). Further analyses considered anterior and posterior gills separately, and the results showed that posterior gills are the main tissues fueling osmoregulatory-related processes because their respiration rates in dSW were 3.2-fold higher than those of anterior gills, and this was accompanied by an increase in mitochondrial density (citrate synthase activity) and increased levels of reactive oxygen species (ROS) formation (1.4-fold greater, measured through electron paramagnetic resonance). Paradoxically, these posterior gills showed undisturbed caspase 3/ 7 activity, used here as a marker for apoptosis. This may only be due to the high antioxidant protection that posterior gills benefit from [superoxide dismutase (SOD) in posterior gills was over 6 times higher than in anterior gills]. In conclusion, osmoregulating posterior gills are better adapted to dSW exposure than respiratory anterior gills because they are capable of controlling the deleterious effects of the ROS production resulting from this salinity-induced stress.

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Section: Discussionmentioning

confidence: 94%

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Rivera‐Ingraham

Barri

Boël

et al. 2015

Journal of Experimental Biology

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“…In the current study, the activity of Na + /K + -ATPase and its mRNA expression level in the posterior gills decreased significantly with increasing salinity, similar results was shown for E . sinensis subjected to short-term salinity changes [44–45] as well as in several marine crabs [46–47]. Such a result could be explained by the fact that at higher salinities, particularly 18 ‰, osmolality of hemolymph is very similar to that of external media than in the case of freshwater (e.g.…”

Section: Discussionmentioning

confidence: 99%

Effects of salinity on gonadal development, osmoregulation and metabolism of adult male Chinese mitten crab, Eriocheir sinensis

Long

Zhao

et al. 2017

PLoS ONE

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As a catadromous species, salinity is a key parameter that affects gonadal development of Chinese mitten crab Eriocheir sinensis during reproductive migration. It is however unclear the effects of salinity on the gonadal development of male E. sinensis as well as their physiological responses to salinity during reproductive migration. This study investigated the effects of four salinities (0 ‰, 6 ‰, 12 ‰ and 18 ‰) on gonadal development, osmoregulation and metabolism of adult male E. sinensis over a 40-day period. The results showed that elevating salinity promote gonadal development, increase hemolymph osmolality and K+ and Mg2+ concentrations (P < 0.05). The 12 ‰ salinity resulted in the highest contents of taurine and arginine in the hemolymph while the highest contents of threonine, phenylalanine, lysine, ß-alanine, tryptophan, ornithine and total free amino acids were found for 0 ‰ treatment (P < 0.05). A decreasing trend was detected for the Na+/K+-ATPase activity and its mRNA expression level in the posterior gills with salinity (P < 0.05). Total saturated fatty acids in the anterior gills decreased with increasing salinity (P < 0.05); the 0 ‰ treatment had the highest total polyunsaturated fatty acids in the posterior gills while total n-6 polyunsaturated fatty acids increased with salinity (P < 0.05). The hemolymph glucose and uric acid showed a decreasing trend as salinity while an increasing trend was found for the hemolymph triglyceride and high-density lipoprotein cholesterol (P < 0.05). The 12 ‰ treatment had the highest levels of hemolymph malonaldehyde and hepatopancreatic γ-glutamyltranspeptidase (P < 0.05). In conclusion, these results suggested that the brackish water promote gonadal development of male E. sinensis, and increase osmolality and ionic concentrations in hemolymph while reduced the activity of Na+ /K+- ATPase and its mRNA expression in the posterior gills as well as metabolism.

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“…NKCC is an integral membrane protein responsible for the simultaneous transport of one Na + , one K + , and two Cl − ions from the external to the internal side of epithelial cells (Kang et al 2010). Although previous studies suggest that NKCC plays a critical role in the osmoregulatory endurance of fish (Kang et al 2010;Watson et al 2014;LorinNebel et al 2006;Ip et al 2013;Chandrasekar et al 2014), its role in salinity adaptations has been little studied in crustaceans compared to other ion transport genes (i.e., Na + , K + -ATPase, V-type H + -ATPase, carbonic anhydrase (CA)) (Tsai and Lin 2007;Lucu et al 2008;Firmino et al 2011;Han et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of salinity acclimation and eyestalk ablation on Na+, K+, 2Cl− cotransporter gene expression in the gill of Portunus trituberculatus:a molecular correlate for salt-tolerant trait

Zhang

Ping

et al. 2016

Cell Stress and Chaperones

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The Na− cotransporter (NKCC) is an important gene in ion transport. In order to elucidate its function, and regulatory mechanisms, in salinity acclimation, the complete cDNA sequence of NKCC (4218 bp) from Portunus trituberculatus (PtNKCC) was first cloned and characterized. It was found to encode 1055 amino acids containing conserved AA-permease and SLC12 motifs. Results show that PtNKCC is expressed to the greatest extent in gills. High salinity stress exposure led to significant increases (9.6-fold) of PtNKCC mRNA expression in the gills 12 h after treatment, declining to less than the levels seen in the control group between 48 and 72 h. During low salinity stress, expression levels of PtNKCC in gills were found to be upregulated at each sampling time, reaching their peak after 6 h (a 12.4-fold increase). Eyestalk ablation also triggered an 11.3-fold increase in PtNKCC mRNA, while re-injection with eyestalk homogenates significantly reduced the expression of PtNKCC mRNA. Four single nucleotide polymorphisms (SNPs) were detected in the PtNKCC open reading frame, and one SNP was associated with salt tolerance. Our results indicate that PtNKCC plays an important role in the salinity acclimation of P. trituberculatus, while there may be a compound present in the XOSG that inhibits the expression of PtNKCC.

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Changes in Na+/K+-ATPase activity, unsaturated fatty acids and metallothioneins in gills of the shore crab Carcinus aestuarii after dilute seawater acclimation

Cited by 38 publications

References 56 publications

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?

Effects of salinity on gonadal development, osmoregulation and metabolism of adult male Chinese mitten crab, Eriocheir sinensis

Effects of salinity acclimation and eyestalk ablation on Na+, K+, 2Cl− cotransporter gene expression in the gill of Portunus trituberculatus:a molecular correlate for salt-tolerant trait

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