Changes in Lens Proteins of Rats During Aging

Biochemistry of the Ageing Rat Lens

“…Id). Dische et al [1956], Lerman and Zigman [1967], Lerman [1969], Sinha [1969] and Zigman et al [1970] found in the rat lens the same tendency. In other species like mouse, man, ox and dog the same phenomenon was also observed [Bours, 1980a].…”

Biochemistry of the Ageing Rat Lens

“…Id). Dische et al [1956], Lerman and Zigman [1967], Lerman [1969], Sinha [1969] and Zigman et al [1970] found in the rat lens the same tendency. In other species like mouse, man, ox and dog the same phenomenon was also observed [Bours, 1980a].…”

Cloning and Overexpression of the Xylitol Dehydrogenase Gene from Bacillus pallidus and Its Application to L-Xylulose Production

“…The recombinant xylitol dehydrogenase (XDH) was purified to homogeneity by three-step column chromatography, producing a single SDS-PAGE band of 28 kDa apparent molecular mass. The enzyme exhibited maximal activity at 55 C in glycine-NaOH buffer pH 11.0, with 66% of initial enzyme activity retained after incubation at 40 C for 1 h. In further application of the recombinant bacterium to L-xylulose production from xylitol (initial concentration 5%) using a resting cell reaction, 35% L-xylulose was produced within 24 h. This result indicates that this recombinant XDH is applicable in the large-scale production of L-xylulose.…”

Surface‐Layer Lattices as Patterning Element for Multimeric Extremozymes

“…The xylose isomerase activity of rXylA and of the rSbpA/XylA fusion protein was measured spectrophotometrically using glucose as substrate and the converted fructose was determined by the cysteine carbazole sulfuric acid method 43. Starting with a pH and temperature screening (data not shown), the optimal test conditions were determined, and revealed, in line with previous findings,25 that the highest enzyme activity for the sole and the S‐layer‐fused enzyme was obtained at pH 6.5 and 80 °C ( Table 1 ).…”

Biochemistry of the Ageing Rat Lens

“…Id). Dische et al [1956], Lerman and Zigman [1967], Lerman [1969], Sinha [1969] and Zigman et al [1970] found in the rat lens the same tendency. In other species like mouse, man, ox and dog the same phenomenon was also observed [Bours, 1980a].…”

Cloning and Overexpression of the Xylitol Dehydrogenase Gene from Bacillus pallidus and Its Application to L-Xylulose Production

“…The recombinant xylitol dehydrogenase (XDH) was purified to homogeneity by three-step column chromatography, producing a single SDS-PAGE band of 28 kDa apparent molecular mass. The enzyme exhibited maximal activity at 55 C in glycine-NaOH buffer pH 11.0, with 66% of initial enzyme activity retained after incubation at 40 C for 1 h. In further application of the recombinant bacterium to L-xylulose production from xylitol (initial concentration 5%) using a resting cell reaction, 35% L-xylulose was produced within 24 h. This result indicates that this recombinant XDH is applicable in the large-scale production of L-xylulose.…”

Surface‐Layer Lattices as Patterning Element for Multimeric Extremozymes

“…The xylose isomerase activity of rXylA and of the rSbpA/XylA fusion protein was measured spectrophotometrically using glucose as substrate and the converted fructose was determined by the cysteine carbazole sulfuric acid method 43. Starting with a pH and temperature screening (data not shown), the optimal test conditions were determined, and revealed, in line with previous findings,25 that the highest enzyme activity for the sole and the S‐layer‐fused enzyme was obtained at pH 6.5 and 80 °C ( Table 1 ).…”

Biochemistry of the Ageing Rat Lens

“…Id). Dische et al [1956], Lerman and Zigman [1967], Lerman [1969], Sinha [1969] and Zigman et al [1970] found in the rat lens the same tendency. In other species like mouse, man, ox and dog the same phenomenon was also observed [Bours, 1980a].…”

Cloning and Overexpression of the Xylitol Dehydrogenase Gene from Bacillus pallidus and Its Application to L-Xylulose Production

“…The recombinant xylitol dehydrogenase (XDH) was purified to homogeneity by three-step column chromatography, producing a single SDS-PAGE band of 28 kDa apparent molecular mass. The enzyme exhibited maximal activity at 55 C in glycine-NaOH buffer pH 11.0, with 66% of initial enzyme activity retained after incubation at 40 C for 1 h. In further application of the recombinant bacterium to L-xylulose production from xylitol (initial concentration 5%) using a resting cell reaction, 35% L-xylulose was produced within 24 h. This result indicates that this recombinant XDH is applicable in the large-scale production of L-xylulose.…”

Surface‐Layer Lattices as Patterning Element for Multimeric Extremozymes

“…The xylose isomerase activity of rXylA and of the rSbpA/XylA fusion protein was measured spectrophotometrically using glucose as substrate and the converted fructose was determined by the cysteine carbazole sulfuric acid method 43. Starting with a pH and temperature screening (data not shown), the optimal test conditions were determined, and revealed, in line with previous findings,25 that the highest enzyme activity for the sole and the S‐layer‐fused enzyme was obtained at pH 6.5 and 80 °C ( Table 1 ).…”