Changes in ionized calcium concentration in the blood of dairy cows with peracute coliform mastitis

Hisaeda, Keiichi; Koshiishi, Tomoko; Sasaki, Ayuna; Shinozuka, Yasunori; Isobe, Naoki; Kawai, Kazuhiro

doi:10.1292/jvms.19-0678

Cited by 8 publications

(8 citation statements)

References 29 publications

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“…Three calcium homeostasis modulator genes (CALHM1, CALHM2, and CALHM3) are found in the list of positional candidate genes contributing to the GO term (Supplemental Table S3, https: / / doi .org/ 10 .3168/ jds .2020 -18209). The high ranking of these candidate genes is in line with the observation that the calcium concentration in the blood of dairy cows with and without mastitis differs, which could be an indicator for metabolic imbalance (Wegner and Stull, 1978;Hisaeda et al, 2020). No other significant overrepresentation was detected using GO.…”

Section: Markers For CM In Dsnsupporting

confidence: 74%

A genome-wide association study for clinical mastitis in the dual-purpose German Black Pied cattle breed

Meier

Arends

Korkuć

et al. 2020

Journal of Dairy Science

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The dual-purpose German Black Pied Cattle (DSN) has become an endangered breed of approximately 2,550 registered cows in Germany. The breed is genetically related to Holstein-Friesian cattle because the old DSN breed contributed to the selection of the modern Holstein dairy cow. In dairy farms, breeders aim to improve animal health and well-being by reducing the number of mastitis cases, which would also reduce milk losses and treatment costs. On the genomic level, no markers associated with clinical mastitis have been reported in DSN. Therefore, we performed a genome-wide association study on 1,062 DSN cows using a univariate linear mixed model that included a relatedness matrix to correct for population stratification. Although the statistical power was limited by the small population size, 3 markers were significantly associated, and 2 additional markers showed a suggestive association with clinical mastitis. Those markers accounted for 1 to 3% of the variance of clinical mastitis in the examined DSN population. One marker was found in the intragenic region of NEURL1 on BTA26, and the other 4 markers in intergenic regions on BTA3, BTA6, and BTA9. Further analyses identified 23 positional candidate genes. Among them is BMPR1B, which has been previously associated with clinical mastitis in other dairy cattle breeds. The markers presented here can be used for selection for mastitis-resistant animals in the endangered DSN population, and can broadly contribute to a better understanding of mastitis determinants in dairy cattle breeds.

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Section: Markers For CM In Dsnsupporting

confidence: 74%

A genome-wide association study for clinical mastitis in the dual-purpose German Black Pied cattle breed

Meier

Arends

Korkuć

et al. 2020

Journal of Dairy Science

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“…However, there are no published data for EDAN, neither for validation nor for diagnostic performance. In general, the mean iCa concentrations analyzed by GEM and EDAN corresponded to those reported in the literature for dairy cows at different stages of lactation (1.02‐1.20 mmol/L), especially between calving and PP3 4,8,15,38 . This finding showed that there were no large and abnormal deviations in the iCa analysis of both GEM and EDAN compared to the previously published data.…”

Section: Discussionsupporting

confidence: 84%

“…9 The present study investigated the diagnostic performance of EDAN compared to GEM based on multiple cut-off points for serum TCa and ICA. This was different from most recent studies on dairy cows, 3,4,19,38 but in line with others regarding the use of serum TCa as a reference. 19 The cut-off points of serum TCa <2.15 9,18,28 and 2.00 7,19,20,24 mmol/L for subclinical hypocalcemia were recently defined by others and used in the analysis with ROC curve for the diagnostic performance of GEM and EDAN in the present study.…”

Section: Discussioncontrasting

confidence: 63%

Validation of a blood gas device for ionized calcium analysis in Holstein cows

Denız¹,

Metin

Aksoy

et al. 2023

Veterinary Clinical Pathol

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Background: Accurate analysis of ionized calcium (iCa) is critical for the detection of hypocalcemia or subclinical hypocalcemia. The Edan i15 Vet (EDAN) blood gas device has not been validated for iCa in dairy cows. Objectives:We aimed to validate the EDAN blood gas device against the Gem Premier 3000 (GEM) analyzer by measuring iCa concentrations and evaluating the ability of these measurements, compared with serum total calcium (TCa) concentrations, to diagnose subclinical hypocalcemia.Methods: iCa concentrations were measured with the EDAN and GEM devices, and serum TCa concentrations were measured with a wet biochemistry method with blood from 125 lactating Holstein cows between calving to day 27 postpartum.Results: Bland-Altman plots showed a mean and total bias of 0.05 and 0.24 mmol/L for the EDAN device, respectively. The intercept did not include zero, but the slope included 1.0 in the Passing-Bablok regression. The sensitivity and specificity (Se/Sp) of the EDAN device were 93/94%, 93/90%, 91/93%, and 85/95% for iCa cut-off values of <1.00, 1.05, 1.10, and 1.15 mmol/L, respectively, as determined with the GEM device. The Se/Sp were 57/82% and 72/80% for EDAN and 57/80% and 72/79% for GEM at serum TCa cut-off points <2.15 and <2.00 mmol/L, respectively. The average iCa concentrations analyzed with the GEM and EDAN devices were 1.04 ± 0.18 and 1.09 ± 0.17, respectively. Conclusions:The EDAN device did not have satisfactory agreement with GEM and could not be used interchangeably, but it had satisfactory Se/Sp to diagnose subclinical hypocalcemia compared with the GEM-derived iCa cut-off points. Serum TCa concentration cut-off values were not suitable for diagnosing subclinical hypocalcemia because of unsatisfactory Se/Sp compared with iCa concentrations analyzed by the GEM and EDAN devices. The iCa values analyzed using the EDAN and GEM devices were consistent with previously reported data.

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“…In a previous study, we observed a positive correlation between serum ionized Ca and Alb concentrations at the time of initial diagnosis in cows wit h acute mastitis [12]. The findings of the present study showed that there were positive correlations between mean serum Alb, Ca, iP, and Mg concentrations.…”

Section: Grünwaldt Et Al Reported That the Serum Globulin And Albumin...supporting

confidence: 72%

The relationships between environmental parameters in livestock pen and physiological parameters of Holstein dairy cows

Nohara

Hisaeda

Ono

et al. 2022

The Journal of Veterinary Medical Science

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There has been an increase in temperature and the incidence of extreme weather events, such as heat wave, due to global warming, which has promoted the incidence of livestock diseases. Therefore, it is important to examine the effect of changes in environmental parameters on livestock performance. The aim of this study was to examine the relationship between ambient environmental conditions in livestock pen and the physiological parameters of Holstein dairy cows. The results showed that there was a decrease in the red blood cell counts, hemoglobin concentrations, and mean corpuscular hemoglobin concentration of the cows with increasing pen temperature, wet bulb globe temperature (WBGT), and temperature humidity index (THI). Additionally, high daily variation in temperature caused a decrease in the serum albumin levels of the cows. Moreover, the lowest serum calcium, inorganic phosphorus, and magnesium concentrations were observed in November, and were negatively correlated with the 24 -hr temperature, WBGT, and THI range of the pen prior to sampling. Multiple regression analysis showed a positive correlation between serum cortisol concentration and 24 -hr WBGT range of the pen prior to samplings and packed cell volume. However, serum cortisol and total protein c oncentrations were negatively correlated. Overall, the findings of the study suggest that large variation in temperature induced stress in the cows, which could be overcome by increased water consumption and improved protein digestion and absorption by the animals, and the addition of minerals, such as calcium to the diet. KEY WORD blood examination values, cortisol, Holstein dairy cows, temperature humidity index , wet bulb globe temperature .

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Changes in ionized calcium concentration in the blood of dairy cows with peracute coliform mastitis

Cited by 8 publications

References 29 publications

A genome-wide association study for clinical mastitis in the dual-purpose German Black Pied cattle breed

A genome-wide association study for clinical mastitis in the dual-purpose German Black Pied cattle breed

Validation of a blood gas device for ionized calcium analysis in Holstein cows

The relationships between environmental parameters in livestock pen and physiological parameters of Holstein dairy cows

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