Changes in gut microbiota composition with age and correlations with gut inflammation in rats

Chen, Meng; Feng, Siyuan; Hao, Zikai; Dong, Chen; Liu, Hong

doi:10.1371/journal.pone.0265430

Cited by 22 publications

(14 citation statements)

References 45 publications

(33 reference statements)

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“…Healthy and strong gut macrobiotics are critical for growth and weight gain, which shows that multifaceted macronutrient digestion by metabolizing SCFA influences the composition of the gut microbiota with specific changes to the major macronutrients contained in the diet. Age-related changes had an impact; the relative abundance of Firmicutes increased with age, and the relative abundance of Bacteroides decreased with age [ 74 , 75 , 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicity and Impact of Silica Nanoparticles on the Configuration of Gut Microbiota in Immunodeficient Mice

Shabbir

et al. 2023

Microorganisms

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Nanoparticles (NPs), having exceptional physicochemical and electrical characteristics with lower toxicity, have evolved as dynamic drug delivery carriers in living organisms. Potentially, the intragastric gavage of silica nanoparticles (SiNPs) affects gut microbiota profiles in immunodeficient mice. In this study, the impact of SiNPs of variable size and dosage was investigated in cyclophosphamide (Cy)-induced immunodeficient mice, specifically on their immune functions and gut microbiota, through physicochemical and metagenomic analysis. SiNPs of different sizes and doses were gavaged to Cy-induced immunodeficient mice for 12 days at an interval of 24 h to investigate their effects on immunological functions and the gut microbiome of mice. Our results showed that SiNPs had no significant toxicological effects on the cellular and hematological activities of immunodeficient mice. Furthermore, after the administration of different levels of SiNPs, no immune dysfunction was found in the immunosuppressed mice groups. However, gut-microbial studies and comparisons of characteristic bacterial diversity and compositions demonstrated that SiNPs significantly affect the abundance of different bacterial communities. LEfSe analysis revealed that SiNPs significantly increased the abundance of Lactobacillus, Sphingomonas, Sutterella, Akkermansia, and Prevotella, and potentially reduced Ruminococcus and Allobaculum. Thus, SiNPs significantly regulate and modify the configuration of the gut microbiota in immunodeficient mice. These dynamic variations in the intestinal bacterial community, abundance, and diversity provide new insight into the regulation and administration of silica-based NPs. This would be helpful for the further demonstration of the mechanism of action and prediction of the potential effects of SiNPs.

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Section: Discussionmentioning

confidence: 99%

Toxicity and Impact of Silica Nanoparticles on the Configuration of Gut Microbiota in Immunodeficient Mice

Shabbir

et al. 2023

Microorganisms

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“…While Alistipes relative abundance is increased in prediabetic and diabetic patients and in hyperglycemic mice, a causal role for increased Alistipes in T2D has not been established [ 50 , 51 , 52 ]. Ruminococcus is also increased in prediabetic and diabetic patients, and although its role in diabetes development is unclear, increases in Ruminococcus are associated with increased intestinal inflammation which could lead to gut barrier dysfunction and further metabolic consequences, potentially initiating disease development [ 53 , 54 , 55 ]. Conversely, the genera Blautia , Lachnospiraceae UCG-001 , and Roseburia were shifted in only the diabetic state, indicating that host physiological changes may be influencing their relative abundance, highlighting their potential to serve as markers for T2D.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal Characterization of the Gut Microbiota in the Diabetic ZDSD Rat Model and Therapeutic Potential of Oligofructose

et al. 2023

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The complex development of type 2 diabetes (T2D) creates challenges for studying the progression and treatment of the disease in animal models. A newly developed rat model of diabetes, the Zucker Diabetic Sprague Dawley (ZDSD) rat, closely parallels the progression of T2D in humans. Here, we examine the progression of T2D and associated changes in the gut microbiota in male ZDSD rats and test whether the model can be used to examine the efficacy of potential therapeutics such as prebiotics, specifically oligofructose, that target the gut microbiota. Bodyweight, adiposity, and fed/fasting blood glucose and insulin were recorded over the course of the study. Glucose and insulin tolerance tests were performed, and feces collected at 8, 16, and 24 weeks of age for short-chain fatty acids and microbiota analysis using 16s rRNA gene sequencing. At the end of 24 weeks of age, half of the rats were supplemented with 10% oligofructose and tests were repeated. We observed a transition from healthy/nondiabetic to prediabetic and overtly diabetic states, via worsened insulin and glucose tolerance and significant increases in fed/fasted glucose, followed by a significant decrease in circulating insulin. Acetate and propionate levels were significantly increased in the overt diabetic state compared to healthy and prediabetic. Microbiota analysis demonstrated alterations in the gut microbiota with shifts in alpha and beta diversity as well as alterations in specific bacterial genera in healthy compared to prediabetic and diabetic states. Oligofructose treatment improved glucose tolerance and shifted the cecal microbiota of the ZDSD rats during late-stage diabetes. These findings underscore the translational potential of ZDSD rats as a model of T2D and highlight potential gut bacteria that could impact the development of the disease or serve as a biomarker for T2D. Additionally, oligofructose treatment was able to moderately improve glucose homeostasis.

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“…Despite the recommendations to take into account possible dynamic processes in the microbiota composition of intact animals [ 35 ], in most studies, stool sampling for analysis is done in the control and experimental group once: for illustration [ 36 , 37 ]. At the same time, there is evidence of significant changes in alpha diversity indexes in intact mature rodents kept under standard conditions if biomaterial sampling is carried out every week [ 38 ]. Taking to account for these changes, we carried out sampling not only in animals of the control group at 3 time points, but also in the experimental group before stress.…”

Section: Discussionmentioning

confidence: 99%

Post-stress changes in the gut microbiome composition in rats with different levels of nervous system excitability

Shevchenko,

Shalaginova,

Katserov

et al. 2023

PLoS ONE

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The gut-brain axis is a critical communication system influencing the interactions between the gastrointestinal tract (GI) and the central nervous system (CNS). The gut microbiota plays a significant role in this axis, affecting the development and function of the nervous system. Stress-induced psychopathologies, such as depression and anxiety, have been linked to the gut microbiota, but underlying mechanisms and genetic susceptibility remain unclear. In this study, we examined stress-induced changes in the gut microbiome composition in two rat strains with different levels of nervous system excitability: high threshold (HT strain) and low threshold (LT strain). Rats were exposed to long-term emotional and painful stress using the Hecht protocol, and fecal samples were collected at multiple time points before and after stress exposure. Using 16S rRNA amplicon sequencing, we assessed the qualitative and quantitative changes in the gut microbiota. Our results revealed distinct microbial diversity between the two rat strains, with the HT strain displaying higher diversity compared to the LT strain. Notably, under prolonged stress, the HT strain showed an increase in relative abundance of microorganisms from the genera Faecalibacterium and Prevotella in fecal samples. Additionally, both strains exhibited a decrease in Lactobacillus abundance following stress exposure. Our findings provide valuable insights into the impact of hereditary nervous system excitability on the gut microbiome composition under stress conditions. Understanding the gut-brain interactions in response to stress may open new avenues for comprehending stress-related psychopathologies and developing potential therapeutic interventions targeted at the gut microbiota. However, further research is needed to elucidate the exact mechanisms underlying these changes and their implications for stress-induced disorders. Overall, this study contributes to the growing body of knowledge on the gut-brain axis and its significance in stress-related neurobiology.

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Changes in gut microbiota composition with age and correlations with gut inflammation in rats

Cited by 22 publications

References 45 publications

Toxicity and Impact of Silica Nanoparticles on the Configuration of Gut Microbiota in Immunodeficient Mice

Toxicity and Impact of Silica Nanoparticles on the Configuration of Gut Microbiota in Immunodeficient Mice

Longitudinal Characterization of the Gut Microbiota in the Diabetic ZDSD Rat Model and Therapeutic Potential of Oligofructose

Post-stress changes in the gut microbiome composition in rats with different levels of nervous system excitability

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