Changes in free amino acids in the hemolymph of giant freshwater prawn Macrobrachium rosenbergii exposed to varying salinities: relationship to osmoregulatory ability

Hương, Đỗ Thị Thanh; Yang, Wei‐Jun; Okuno, Akira; Wilder, Marcy N.

doi:10.1016/s1095-6433(00)00310-x

Cited by 73 publications

(52 citation statements)

References 19 publications

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“…Such tissue-specific responses evidently reflect the efficient hemolymph osmotic and ionoregulatory mechanisms and reveal the dependence of muscle tissue on intracellular osmotic effectors like FAA. This response is consistent with the regulatory physiology Glycine, proline, alanine and arginine are the main effectors of intracellular isosmotic regulation in most crustaceans and constitute 40 to 60% of total FAA concentration, independently of salinity, ontogenetic stage or habitat (Dalla Via 1986, Haond et al 1999, Huong et al 2001, Wang et al 2004, Augusto et al 2007a. In Palaemon northropi, the decrease in muscle total FAA on acclimation to 5 ‰ is mainly a consequence of diminished glycine, arginine and proline, confirming that these nitrogenous components play a significant role in regulating intracellular volume.…”

Section: Discussionsupporting

confidence: 83%

“…FAA titers were higher in Palaemon northropi than in diadromous freshwater palaemonids like Macrobrachium amazonicum and M. olfersi (164 and 188 nmol mg -1 DW, respectively; Augusto et al 2007a) and M. rosenbergii (~390 nmol mg -1 DW; Huong et al 2001). Further, in M. brasiliense, a hololimnetic inhabitant of inland continental rivers that exhibits abbreviated larval development, FAA concentrations (142 nmol mg -1 DW) are lower than in other Macrobrachium species (Faria et al 2008).…”

Section: Discussionmentioning

confidence: 96%

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Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Augusto¹,

Pinheiro²,

Greene³

et al. 2009

Aquat. Biol.

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The transition from marine/brackish waters to freshwater habitats constitutes a severe osmotic and ionic challenge, and successful invasion has demanded the selection of morphological, physiological, biochemical and behavioral adaptations. We evaluated short-term (1 to 12 h exposure) and long-term (5 d acclimation), anisosmotic extracellular (osmolality, [Na + , Cl -]) and long-term isosmotic intracellular osmoregulatory capability in Palaemon northropi, a neotropical intertidal shrimp. P. northropi survives well and osmo-and ionoregulates strongly during short-and long-term exposure to 5-45 ‰ salinity, consistent with its rocky tide pool habitat subject to cyclic salinity fluctuations. Muscle total free amino acid (FAA) concentrations decreased by 63% in shrimp acclimated to 5 ‰ salinity, revealing a role in hypoosmotic cell volume regulation; this decrease is mainly a consequence of diminished glycine, arginine and proline. Total FAA contributed 31% to muscle intracellular osmolality at 20 ‰, an isosmotic salinity, and decreased to 13% after acclimation to 5 ‰. Gill and nerve tissue FAA concentrations remained unaltered. These tissue-specific responses reflect efficient anisosmotic and anisoionic extracellular regulatory mechanisms, and reveal the dependence of muscle tissue on intracellular osmotic effectors. FAA concentration is higher in P. northropi than in diadromous and hololimnetic palaemonids, confirming muscle FAA concentration as a good parameter to evaluate the degree of adaptation to dilute media. The osmoregulatory capability of P. northropi may reflect the potential physiological capacity of ancestral marine palaemonids to penetrate into dilute media, and reveals the importance of evaluating osmoregulatory processes in endeavors to comprehend the invasion of dilute media by ancestral marine crustaceans.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 96%

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Augusto¹,

Pinheiro²,

Greene³

et al. 2009

Aquat. Biol.

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“…The presently reported FAA, including proline, glycine and taurine, belong to the same general set of AA that are involved in osmoregulation and cell volume regulation in marine invertebrates (Hahnenkamp & Fyhn 1985, Huong et al 2001) and also in freshwater crustaceans such as the prawn Macrobrachium rosenbergii (Huong et al 2001). These AA often dominate the FAA pool and have been shown to increase dramatically with an increase in salinity as part of the osmoconforming strategy (Hahnenkamp & Fyhn 1985, Huong et al 2001. Further, these AA are typically simple, indispensable AA, and can also serve as energy substrates.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of two vitellogenins in the salmon louse Lepeophtheirus salmonis: molecular, functional and evolutional analysis

Dalvin¹,

Frost²,

Loeffen³

et al. 2011

Dis. Aquat. Org.

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The salmon louse Lepeophtheirus salmonis Krøyer affects a variety of wild salmonoid hosts, but is also an important pest in aquaculture, which is a globally important and rapidly growing industry. Salmon lice have large reproductive outputs, and knowledge of reproductive processes may be crucial for the control of this parasite. Here, we report on the characterisation of 2 vitellogenins (LsVit1 and LsVit2), which are the precursors of salmon-louse egg-yolk glycoprotein. The structure of LsVit1 and LsVit2 was examined and compared to that in other oviparous animals. Phylogenetic analysis of LsVit1 and LsVit2 confirmed the view that crustaceans are a polyphyletic group. Transcriptional and translational analysis demonstrated production of LsVit1 and LsVit2 in the subcuticular tissue of the adult female lice. LsVit1 and LsVit2 could also be found in maturing oocytes and developing embryos and early larval stages. LsVit2 was found to be processed into 2 smaller fragments, whereas LsVit1 was found to be full length when deposited into the oocytes. Degradation of LsVit1 and LsVit2 was characterised through embryogenesis and the early non-feeding larval stages. Finally, protein content and the level of free amino acids were analysed in embryos and larval stages and their role in nutrition and osmoregulation discussed. In conclusion, our results confirm the role of vitellogenins in reproduction as providers of embryonic and larval nutrition.

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“…Current researches concerning physiological mechanism of crustacean osmoregulation include: structure of gill and antennal gland (Barraet et al, 1983;Lin, 2000), ion transport enzymes and ion transport of branchial epithelium (Castiho et al, 2001;Chen and Chia, 1997;Lima et al, 1997;Lucu and Devescovi, 1999;Lucu et al, 2000;Morris and Edwards, 1995;Onken and Riestenpatt, 1998), hemolymph composition and osmoregulation (Huong et al, 2001;Wilder and Ikuta, 1998), and neuroendocrine control (Freire et al, 1995;Morris, 2001). Many researchers have studied the membrance potential and osmoregulation of isolated perfused gill epithelium Gilles, 1977;Péqueux and Gilles, 1978;Péqueux and Gilles, 1981;Péqueux and Gilles, 1988), effects of osmotic pressure on protein concentration and amino acid content of hemolymph (Freire et al, 1995;Huong et al, 2001). However, at present, there are only a few studies on the effects of environmental factors such as salinity, pH and ionic component on the osmoregulation of crustacea such as E. sinensis Riestenpatt et al, 1994;Trausch et al, 1989;Lü et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Effect of salinity on hemolymph osmotic pressure, sodium concentration and Na+-K+-ATPase activity of gill of Chinese crab, Eriocheir sinensis

Liu

Pan

2008

J. Ocean Univ. China

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The effects of salinity on hemolymph osmotic pressure, Na + concentration and Na + -K + -ATPase activity of gill of Chinese crab Eriocheir sinensis were studied. The results showed that hemolymph osmotic pressure and Na + concentration increased significantly (P<0.05), and the Na + -K + -ATPase activity of gills decreased significantly (P<0.05) when salinity increased from 0 to 16. The hemolymph osmotic pressure and Na + concentration in each treatment group rose remarkably at 0.125 d or 0.25 d, while the Na + -K + -ATPase activity of gill reduced gradually with increased experiment time in 3 d. Then the three parameters remained at a constant level after 0.25 d, 0.125 d and 3 d, respectively, and higher hemolymph osmotic pressure, higher Na + concentration and lower Na + -K + -ATPase activity of gill occurred at higher salinity. The effect of salinity change on protein concentration of hemolymph was indistinct (P>0.05); However, the protein concentration decreased gradually with the increase of salinity from 0.25 d to 1 d, and then tended to be stable from day 1 to day 15.

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Changes in free amino acids in the hemolymph of giant freshwater prawn Macrobrachium rosenbergii exposed to varying salinities: relationship to osmoregulatory ability

Cited by 73 publications

References 19 publications

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Characterisation of two vitellogenins in the salmon louse Lepeophtheirus salmonis: molecular, functional and evolutional analysis

Effect of salinity on hemolymph osmotic pressure, sodium concentration and Na+-K+-ATPase activity of gill of Chinese crab, Eriocheir sinensis

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