Changes in fertilization and hatching rates with time after ovulation induced by 17, 20β-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica

Ohta, Hiromi; Kagawa, Hirohiko; Tanaka, Hideki; Okuzawa, Koichi; Hirose, Kei

doi:10.1016/0044-8486(95)01167-6

Cited by 153 publications

(97 citation statements)

References 16 publications

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“…The eggs obtained by stripping in these combinations might have remained longer period in ovocoel after ovulation. These ovulated eggs were also exposed to hypoxic condition, led to over ripening (Ohta et al 1996). Hence the high fraction of abnormal larvae at these combinations may have originated from fertilization of over-ripped eggs in this catfish, as also reported in Pangasianodon hypophthalmus (cf.…”

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confidence: 71%

Observation on larval deformity during induced spawning of walking catfish, <I>Clarias batrachus</I> (Actinopterygii: Siluriformes: Clariidae), at different combinations of human chorionic gonadotropin dose and latency period

Sahoo¹,

Giri²,

Chandra³

et al. 2009

Acta Icth et Piscat

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The walking catfish, Clarias batrachus (L.), is a potential species for aquaculture. Its production has been documented at few occasions (Areerat 1987, Thakur andDas 1986). Availability of quality seed is one of the important pre-requisites for successful culture of any species. So induced spawning is always advisable for getting optimum quantity and quality of the stocking material.The breeding performance and egg quality of C. batrachus has been reported by the use of different dose of inducing agents with latency combinations in several occasions (Zonneveld et al. 1988, Sahoo et al. 2005, 2007. The deformed larvae like bend trunk and tail, acephalic and tunicate larvae are often encountered along with the normal larvae during breeding operations in carp (Rath et al. 1995) and catfish (Sahoo et al. 2004). The quantity of deformed larvae in Silurus glanis was observed as high as 50% during hatchery production (Linhart and Billard 1995). The deformity in larvae is originated due to poor egg quality, impact of environment or due to fertilization of over-ripped eggs. The over-ripening of eggs occurs due to delayed in stripping of ovulated eggs. This condition is sometimes happened while working in the hatchery condition. The increase in number of deformed larvae affects the production, quality of larvae and the profitability of a hatchery. No information is available on the pattern of deformed larval production while using human chorionic gonadotropin (hCG) as an inducing agent during induced breeding of C. batrachus. Hence the present study is aimed to communicate the pattern of deformity among the hatchlings during its induced spawning at different dose of hCG and latency period combinations.C. batrachus broods were raised in earthen ponds (0.01 ha) at the Institute and were fed with pelleted feed containing 30% crude protein at 2% of their body weight, daily. The female broods of 120-130 g weight range were selected for induced breeding during July-August. The female broods were selected as suggested earlier (Sahoo et al. 2005). The hCG was purchased and reconstituted in 1 mL of solvent provided with the pack. That was further diluted with normal saline solution to get required concentrations of injectable hCG. The groups of females were injected with 1000, 2000, 3000, 4000, and 5000 IU hCG per kg body weight, and stripped after five latency periods, 11, 14, 17, 20, and 23 h in 25 (5 × 5) different combinations. Each group of fish comprised of five females and, was considered for each dose level and latency period combination.The testes were removed from three male fish, incised and squashed in normal saline solution (0.89% NaCl) to get sperm suspension. A drop of sperm suspension was ACTA ICHTHYOLOGICA ET PISCATORIA (2009) Abstract. Five combinations of human chorionic gonadotropin (hCG) dose and five latency periods were evaluated to observe the pattern of deformed larvae among the hatchlings in walking catfish, Clarias batrachus (L.). A higher percentage (7%-12%) of deformed larvae were observed at 1000, 20...

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mentioning

confidence: 71%

Observation on larval deformity during induced spawning of walking catfish, <I>Clarias batrachus</I> (Actinopterygii: Siluriformes: Clariidae), at different combinations of human chorionic gonadotropin dose and latency period

Sahoo¹,

Giri²,

Chandra³

et al. 2009

Acta Icth et Piscat

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“…The absence of eggs from females which ovulated 10 hours after the first hormonal injection and the increase in fertilization rate a few hours later was an unexpected effect. In experiments carried out with fish, the opposite is usually observed, according to Woynarovich & Horvvath (1983) and Ohta et al (1996).…”

Section: Resultsmentioning

confidence: 99%

“…According to Ohta et al (1996), the evaluation of viability of eggs which were extruded in different periods after ovulation is valid only when total ovulation (simultaneous release of all eggs) occurs. Preliminary studies confirmed that in bullfrogs, ovulation is total.…”

Section: Methodsmentioning

confidence: 99%

Time interval from ovulation to extrusion in female bullfrog in different photoperiods

et al. 2011

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-It was analyzed in this work the influence of photoperiod on time interval from ovulation induction period to extrusion of ovocits in female bullfrogs (Lithobates catesbeianus). It was used 54 females reared from metamorphosis to 9 months of age under three photoperiods: dark time (DL 0:24), 16 hours of daylight (DL 16:8) and 12 hours of daylight (DL 12:12). Ovulation was induced by intramuscular application of two doses of LHRHa with 12 hours of interval between the injections. After 10, 25, 28, 31, 34 and 37 hours from the first hormone injection, 10-gram samples (3,000 eggs) were extracted from each female at each time interval and fertilized. Egg hatching rate was checked in each sample 72 hours after fertilization. Analysis of variance showed a significant effect of extrusion delay and the interaction between photoperiod and this delay. Extrusion should be carried out 33, 24 and 26 hours after the first hormone dosage in females reared in environments without light, with 12 hours of daylight and with 16 hours of daylight, respectively, to obtain the maximum fertilization rate.

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“…The Japanese eel and other anguillid eels migrate at depths of less than about 800 m and show vertical migrations to shallower depths at night [33]. However, the maturation of C. myriaster appears to occur at much colder temperatures of about 6°C [34] than the maturation temperatures of about 19-22°C seen for the Japanese eel [35,36]. This suggests the possibility that C. myriaster migrates at deeper depths than the Japanese eel, and that the Kyushu-Palau Ridge could be a landmark used by this species to locate the area of aggregation and spawning.…”

Section: Current Flow Along the 136°e Linementioning

confidence: 99%

Discovery of a spawning area of the common Japanese conger Conger myriaster along the Kyushu-Palau Ridge in the western North Pacific

et al. 2012

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The common Japanese conger Conger myriaster is an important commercial coastal fisheries species in East Asia, but its spawning area has not been determined. A larval sampling survey was conducted in September 2008 along 136°E between 13°N and 22°N, which roughly followed the Kyushu-Palau Ridge in the western North Pacific. Twenty larval specimens were confirmed to be C. myriaster using DNA analysis. Two were newly hatched larvae (preleptocephali) 5.8 and 7.8 mm in total length (TL), which were caught at 17°N. The 5.8 mm TL larva was estimated to be 3-4 days after hatching, the youngest preleptocephalus (i.e., the earliest stage) of this species ever collected. Eighteen other leptocephali were caught at 18°N and 21°N, and these ranged from 18.6 to 40.0 mm TL. Based on these collections, we discerned that there is a spawning area of C. myriaster in the area along the Kyushu-Palau Ridge approximately 380 km south of Okinotorishima Island. Similar to the Japanese eel spawning area along the West Mariana Ridge, the Kyushu-Palau Ridge may play an important role as a landmark of the spawning area. The discovery of this offshore spawning area should lead us to a better understanding of the recruitment mechanisms of C. myriaster, and help to facilitate future international management efforts.

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Changes in fertilization and hatching rates with time after ovulation induced by 17, 20β-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica

Cited by 153 publications

References 16 publications

Observation on larval deformity during induced spawning of walking catfish, <I>Clarias batrachus</I> (Actinopterygii: Siluriformes: Clariidae), at different combinations of human chorionic gonadotropin dose and latency period

Observation on larval deformity during induced spawning of walking catfish, <I>Clarias batrachus</I> (Actinopterygii: Siluriformes: Clariidae), at different combinations of human chorionic gonadotropin dose and latency period

Time interval from ovulation to extrusion in female bullfrog in different photoperiods

Discovery of a spawning area of the common Japanese conger Conger myriaster along the Kyushu-Palau Ridge in the western North Pacific

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