Changes in fatty acid composition in rat blood and organs after infusion of docosahexaenoic acid ethyl ester

Abstract

An infusible emulsion of docosahexaenoic acid ethyl ester (DHA-EE) was prepared. One hundred milliliters of the emulsion contained 10 g DHA-EE (90% pure). Three milliliters of the emulsion was infused into tail veins of 22 Wistar rats weighing approximately 300 g. They were killed 1, 6, and 24 h and 3 and 7 d after the infusion, and fatty acid composition of various organs and plasma was analyzed along with that of control rats. DHA concentrations reached their peaks within 24 h after DHA infusion in plasma lipid fractions and in the phospholipid fraction of liver and lung. DHA did not increase at all in cardiac phospholipid fraction. However, DHA concentrations increased markedly (from 0.7% to 11%) in the free fatty acid fraction of heart 1 h after the infusion. DHA emulsion might be useful for patients in whom a rapid increment in DHA in tissues is beneficial.

Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits. Effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes.

“…However, this relationship is not always the case in single-administration experiments, whether intravenous (27) or oral (29). In the present study, AA concentrations in the plasma lipid fractions were not reduced at 6 h after the administration of 0.8 g DHA/kg irrespective ofthe administration methods used (Tables I and III).…”

“…These findings probably account for the significant changes in DHA, EPA, and AA concentrations in PMN and, hence, changes in LTB4 production in the 0.8-and 0.4-g DHA groups, but not in the 0.2-g DHA or control group. In this context, it is noteworthy that, according to The reason why we developed infusible DHA emulsion is that an instantaneous increase in plasma DHA concentrations by DHA infusion increases those in various tissues very quickly (27) so that DHA might rapidly take effect. However, we have not compared the two administration methods, namely oral and intravenous, by a similar experimental procedure yet.…”

“…This increment was partly due to contaminating EPA (2%) in the DHA-TG preparation for emulsification and partly due to the retroconversion of infused DHA (30,31 (27) demonstrated that the infusion of DHA ethyl ester emulsion into rats increased the free DHA concentrations in plasma and heart from 0 to 8 and to 10 mol% in 1 h, respec- tively. Taken together with these results, it is likely that this quick method of increasing the free DHA concentrations in both plasma and tissues is useful for the prevention ofventricular fibrillation if used in the very early phase of acute myocardial infarction or during pending infarction.…”

Palmitic acid acutely stimulates glucose uptake via activation of Akt and ERK1/2 in skeletal muscle cells

“…Furthermore, the combination of PA, linoleic acid, oleic acid, and stearic acid had similar effects (supplementary Fig. IE), of which ratio mimicked the fatty acid concentration ratio in rat plasma ( 22 ).…”

Docosahexaenoic Acid Pretreatment Confers Protection and Functional Improvements after Acute Spinal Cord Injury in Adult Rats

“…Our rationale for the pretreatment schedule was based on long-standing pharmacokinetic analysis showing that administration of DHA results in fast incorporation (Yamazaki et al, 1991), and accumulates in neurons and glia up to 7 days after injection (Kabuto et al, 2009). Since our initial objective was to investigate the effects of DHA on the inflammatory responses in advance of neurodegenerative trauma, we used a pretreatment schedule that is based on routine interventions for inflammatory conditions, such as the use of methotrexate for rheumatoid arthritis (Cronstein, 2005).…”