Changes in ecdysteroids in Astacus leptodactylus during the molting cycle

The moult cycle of Jasus lalandii (H. Milne Edwards, 1837) (Decapoda, Palinuridae), a cold water spiny lobster species: changes in ecdysteroid titre and setogenesis

“…2). This holds tme for other decapods as well (e.g., Durliat et al, 1988;Snyder & Chang, 1991), and may be due to (i) natural variation within the population; (ii) specific hormonal events within any one stage of the moult cycle, or (iii) an error in assigning the moult stage. The first two possibilities can be addressed by examining the individual change in ecdysteroid levels in the haemolymph over an entire moult cycle.…”

“…lalandii follows the pattem generally found in other cmstaceans, for example the crayfish Astacus leptodactylus (Eschscholtz, 1823) (cf. Durliat et al, 1988), Orconectes sanbomi (Faxon, 1885) (cf. Stevenson et al, 1979), and the lobster H. americanus (cf.…”

The moult cycle of Jasus lalandii (H. Milne Edwards, 1837) (Decapoda, Palinuridae), a cold water spiny lobster species: changes in ecdysteroid titre and setogenesis

“…2). This holds tme for other decapods as well (e.g., Durliat et al, 1988;Snyder & Chang, 1991), and may be due to (i) natural variation within the population; (ii) specific hormonal events within any one stage of the moult cycle, or (iii) an error in assigning the moult stage. The first two possibilities can be addressed by examining the individual change in ecdysteroid levels in the haemolymph over an entire moult cycle.…”

“…lalandii follows the pattem generally found in other cmstaceans, for example the crayfish Astacus leptodactylus (Eschscholtz, 1823) (cf. Durliat et al, 1988), Orconectes sanbomi (Faxon, 1885) (cf. Stevenson et al, 1979), and the lobster H. americanus (cf.…”

Ecdysteroid metabolism in crustaceans

“…The levels of HP conjugates vary over the molt cycle and can at times be the predominant ecdysteroid in the hemolymph [61,77,82,87,88,93]. In H. americanus, the rapid drop in ecdysteroids at the end of premolt is associated with an increase in HP metabolites in the hemolymph and excretion in the urine [93,97].…”

“…The major ecdysteroids identified in the hemolymph of decapod crustaceans are ecdysone, 20E, 3D20E, and PA, but the specific compounds and their levels vary between species, developmental stage, molt stage, and season [32,44,48,49,61,64,66,67,70,78,81,82,87,93,97]. In stone crabs (M. mercenaria) that have been eyestalk ablated to activate the YO, 20E, 3D20E, and PA are present in about equal amounts; a fourth large peak eluting from a reverse-phase column was not identified [32].…”

Expression of cytoskeletal and molt-related genes is temporally scheduled in the hypodermis of the crayfish Procambarus clarkii during premolt

“…The selected genes were responsible for 65±5.1% of the total normalized reads, and the percentage of total induction or attenuation of reads, as well as the Shechter et al (Shechter et al, 2007) and ecdysteroid levels according to Chung (Chung, 2010). The widely accepted Drach index was assigned to P1-P7 by comparison with the findings of Durliat et al (Durliat et al, 1988). The labels D0, D1ʹ-D1ʹʹʹ and D2-D4 mark the approximate starting point of each stage.…”

The moult cycle of Jasus lalandii (H. Milne Edwards, 1837) (Decapoda, Palinuridae), a cold water spiny lobster species: changes in ecdysteroid titre and setogenesis

“…2). This holds tme for other decapods as well (e.g., Durliat et al, 1988;Snyder & Chang, 1991), and may be due to (i) natural variation within the population; (ii) specific hormonal events within any one stage of the moult cycle, or (iii) an error in assigning the moult stage. The first two possibilities can be addressed by examining the individual change in ecdysteroid levels in the haemolymph over an entire moult cycle.…”

“…lalandii follows the pattem generally found in other cmstaceans, for example the crayfish Astacus leptodactylus (Eschscholtz, 1823) (cf. Durliat et al, 1988), Orconectes sanbomi (Faxon, 1885) (cf. Stevenson et al, 1979), and the lobster H. americanus (cf.…”

Ecdysteroid metabolism in crustaceans

“…The levels of HP conjugates vary over the molt cycle and can at times be the predominant ecdysteroid in the hemolymph [61,77,82,87,88,93]. In H. americanus, the rapid drop in ecdysteroids at the end of premolt is associated with an increase in HP metabolites in the hemolymph and excretion in the urine [93,97].…”

“…The major ecdysteroids identified in the hemolymph of decapod crustaceans are ecdysone, 20E, 3D20E, and PA, but the specific compounds and their levels vary between species, developmental stage, molt stage, and season [32,44,48,49,61,64,66,67,70,78,81,82,87,93,97]. In stone crabs (M. mercenaria) that have been eyestalk ablated to activate the YO, 20E, 3D20E, and PA are present in about equal amounts; a fourth large peak eluting from a reverse-phase column was not identified [32].…”

Expression of cytoskeletal and molt-related genes is temporally scheduled in the hypodermis of the crayfish Procambarus clarkii during premolt

“…The selected genes were responsible for 65±5.1% of the total normalized reads, and the percentage of total induction or attenuation of reads, as well as the Shechter et al (Shechter et al, 2007) and ecdysteroid levels according to Chung (Chung, 2010). The widely accepted Drach index was assigned to P1-P7 by comparison with the findings of Durliat et al (Durliat et al, 1988). The labels D0, D1ʹ-D1ʹʹʹ and D2-D4 mark the approximate starting point of each stage.…”

The moult cycle of Jasus lalandii (H. Milne Edwards, 1837) (Decapoda, Palinuridae), a cold water spiny lobster species: changes in ecdysteroid titre and setogenesis

“…2). This holds tme for other decapods as well (e.g., Durliat et al, 1988;Snyder & Chang, 1991), and may be due to (i) natural variation within the population; (ii) specific hormonal events within any one stage of the moult cycle, or (iii) an error in assigning the moult stage. The first two possibilities can be addressed by examining the individual change in ecdysteroid levels in the haemolymph over an entire moult cycle.…”

“…lalandii follows the pattem generally found in other cmstaceans, for example the crayfish Astacus leptodactylus (Eschscholtz, 1823) (cf. Durliat et al, 1988), Orconectes sanbomi (Faxon, 1885) (cf. Stevenson et al, 1979), and the lobster H. americanus (cf.…”

Ecdysteroid metabolism in crustaceans

“…The levels of HP conjugates vary over the molt cycle and can at times be the predominant ecdysteroid in the hemolymph [61,77,82,87,88,93]. In H. americanus, the rapid drop in ecdysteroids at the end of premolt is associated with an increase in HP metabolites in the hemolymph and excretion in the urine [93,97].…”

“…The major ecdysteroids identified in the hemolymph of decapod crustaceans are ecdysone, 20E, 3D20E, and PA, but the specific compounds and their levels vary between species, developmental stage, molt stage, and season [32,44,48,49,61,64,66,67,70,78,81,82,87,93,97]. In stone crabs (M. mercenaria) that have been eyestalk ablated to activate the YO, 20E, 3D20E, and PA are present in about equal amounts; a fourth large peak eluting from a reverse-phase column was not identified [32].…”

Expression of cytoskeletal and molt-related genes is temporally scheduled in the hypodermis of the crayfish Procambarus clarkii during premolt

“…The selected genes were responsible for 65±5.1% of the total normalized reads, and the percentage of total induction or attenuation of reads, as well as the Shechter et al (Shechter et al, 2007) and ecdysteroid levels according to Chung (Chung, 2010). The widely accepted Drach index was assigned to P1-P7 by comparison with the findings of Durliat et al (Durliat et al, 1988). The labels D0, D1ʹ-D1ʹʹʹ and D2-D4 mark the approximate starting point of each stage.…”