Changes in corneal and lens autofluorescence and blood glucose levels in diabetics: parameters of blood glucose control

Methods We measured corneal autofluorescence using a fluorophotometer fitted with an anterior segment adapter.Corneal autofluorescence was measured 10 times at 3 min intervals to evaluate the reproducibility of this instrument in 4 eyes of 4 normal subjects. The diurnal variation in corneal autofluorescence was determined by measuring the fluctuations in 10 eyes in 10 normal subjects and one unoperated eye each of 10 patients with proliferative diabetic retinopathy (PDR). We performed five consecutive measurements at 1000, 1130, 1400, 1630 and 1900 hours. The mean value of five measurements, the variation range and the coefficient of variation were analysed. ResultsThe mean coefficient of variation in the measurement using this instrument was 8.6 ± 1.0%. In the patients with PDR, the mean corneal autofluorescence value was significantly higher (p < 0.001), the variation range was significantly wider (p < 0.001) and the coefficient of variation was significantly greater (p < 0.01) than in the normal subjects. Conclusions The results of this study suggest that corneal autofluorescence changes over the course of a day in patients with diabetes. This may be caused by the breakdown of the blood-aqueous barrier that we reported previously.

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“…17 In the present study, although the sample number was small, no pattern of diurnal variation was found in normal controls, and …”

Section: Pormentioning

confidence: 85%

Diurnal variation of corneal autofluorescence in normal and diabetic eyes

Kitaya

Ishiko

Mori

et al. 1998

Eye

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“…Indeed, there is a positive correlation between HbA1c and fasting blood plasma glucose levels, and separately corneal autofluorescence (Koefoed et al, 1996). High serum glucose concentrations also result in high aqueous glucose levels, especially after the breakdown of the blood-aqueous barrier, which is reported to occur in diabetic patients (Mori et al, 1997). High glucose levels in the aqueous may increase the non-enzymatic glycation of corneal proteins and collagen, thereby increasing the fluorescence of the cornea.…”

Section: Discussionmentioning

confidence: 97%

Autofluorescence Distribution Along the Corneal Axis in Diabetic and Healthy Humans

Schaik

Coppens²,

Berg³

et al. 1999

Experimental Eye Research

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“…We previously showed that corneal autofluorescence values measured using a commercial fluorophotometer significantly increased in patients with proliferative diabetic retinopathy (PDR) (9). Moreover, we reported that lens autofluorescence values using our fluorophotometer were significantly correlated with AGE levels in extracted lenses in diabetic animal models (10).…”

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confidence: 97%

Corneal Advanced Glycation End Products Increase in Patients With Proliferative Diabetic Retinopathy

et al. 2001

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OBJECTIVE—To evaluate corneal advanced glycation end product (AGE) fluorescence in patients with diabetes and in healthy control subjects. RESEARCH DESIGN AND METHODS—Corneal autofluorescence was measured in 26 eyes of 26 patients with type 2 diabetes (mean age 57.0 years; mean disease duration 12.2 years; mean HbA1c 7.1%) and 13 eyes of 13 healthy age-matched control subjects (mean age 57.9 years). The patients with type 2 diabetes were divided into the following groups: patients without diabetic retinopathy (DR), patients without proliferative diabetic retinopathy (PDR), and patients with PDR. Corneal autofluorescence was measured by fluorophotometry with the wavelength that is characteristic of AGE fluorescence (excitation and emission 360–370 nm and 430–450 nm, respectively). We defined peak corneal autofluorescence levels as corneal AGE fluorescence values. We compared the corneal AGE fluorescence values in the four groups. RESULTS—In the PDR group (11.9 ± 3.9 arbitrary units [mean ± SD]), the corneal AGE fluorescence values were significantly higher compared with the control subjects (6.9 ± 1.3 arbitrary units), the patients without DR (7.4 ± 2.1 arbitrary units), and the patients without PDR (6.9 ± 2.2 arbitrary units) (P < 0.05). CONCLUSIONS—We found that corneal AGEs may increase in patients with diabetes and PDR compared with control subjects, patients without DR, and patients without PDR. In the patients with PDR, increased corneal AGEs may play a role in diabetic keratopathy.

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Changes in corneal and lens autofluorescence and blood glucose levels in diabetics: parameters of blood glucose control

Cited by 19 publications

References 18 publications

Diurnal variation of corneal autofluorescence in normal and diabetic eyes

Diurnal variation of corneal autofluorescence in normal and diabetic eyes

Autofluorescence Distribution Along the Corneal Axis in Diabetic and Healthy Humans

Corneal Advanced Glycation End Products Increase in Patients With Proliferative Diabetic Retinopathy

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