Changes in cell proliferation due to environmental non-ionizing radiation

Kwee, Sianette; Raskmark, Povl

doi:10.1016/s0302-4598(97)00095-0

Cited by 73 publications

(36 citation statements)

References 23 publications

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“…This result agrees with published data on microwave exposed human lymphocyte cultures [Antonopoulos et al, 1997]. Though effects on cell proliferation induced by RF ®elds are also reported in the literature, they refer to transformed cell lines (Kwee and Raskmark, 1998;Velizarov et al, 1999).…”

Section: Discussionsupporting

confidence: 92%

Cytogenetic damage in human lymphocytes following GMSK phase modulated microwave exposure

d’Ambrosio

Massa

Scarfì

et al. 2001

Bioelectromagnetics

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The present study investigated, using in vitro experiments on human lymphocytes, whether exposure to a microwave frequency used for mobile communication, either unmodulated or in presence of phase only modulation, can cause modification of cell proliferation kinetics and/or genotoxic effects, by evaluating the cytokinesis block proliferation index and the micronucleus frequency. In the GSM 1800 mobile communication systems the field is both phase (Gaussian minimum shift keying, GMSK) and amplitude (time domain multiple access, TDMA) modulated. The present study investigated only the effects of phase modulation, and no amplitude modulation was applied. Human peripheral blood cultures were exposed to 1.748 GHz, either continuous wave (CW) or phase only modulated wave (GMSK), for 15 min. The maximum specific absorption rate (approximately 5 W/kg) was higher than that occurring in the head of mobile phone users; however, no changes were found in cell proliferation kinetics after exposure to either CW or GMSK fields. As far as genotoxicity is concerned, the micronucleus frequency result was not affected by CW exposure; however, a statistically significant micronucleus effect was found following exposure to phase modulated field. These results would suggest a genotoxic power of the phase modulation per se.

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Section: Discussionsupporting

confidence: 92%

Cytogenetic damage in human lymphocytes following GMSK phase modulated microwave exposure

d’Ambrosio

Massa

Scarfì

et al. 2001

Bioelectromagnetics

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“…It is important to note that regardless the mammalian cells variety, our results are similar to the findings referred by Kwee & Rasmark (1998) who found significant growth suppression of human epithelial amnion cells exposed to 960 MHz, SAR of 0.021, 0.21 and 2.1 W/kg. Since the maximum effect on changes in proliferation rate was found at a specific electromagnetic field frequency and exposure time, the results confirmed hypothesis of so-called "window effect" proposed earlier by Adey (1980).…”

Section: Discussionsupporting

confidence: 91%

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Pavičić

Trošić

2006

Biologia

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Considering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells continuous line V79. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) equipped by Philips PM 5508 signal generator cell samples were sub-cultivated for one day. Cell samples were exposed to 864 MHz continuous wave at an average specific absorption rate (SAR) of 0.08 W/kg. To determine cell growth, V79 cells were plated in concentration of 1 × 10 4 cells per milliliter of nutrient medium RPMI 1640, and raised in a humified atmosphere at 37• C in 5% CO2. Cell proliferation was determined by cell counts for each hour of exposure on post-exposure day 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/mL of RPMI 1640 and incubated according to the deliberated experimental protocol. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion test was used to determine viability of cells. In comparison to sham-exposed cells, growth curve of irradiated cell samples showed significant decrease (p < 0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from exposed "mock" condition. Under strictly controlled laboratory conditions, applied radiofrequency microwaves (RF/MW) irradiation significantly affected cell proliferation kinetics but not viability or ability of V79 cells to form colonies. Sophisticated mechanism of action is intending to be elucidated in the further research which will include insight into the RF/MW related event at the subcellular level.

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“…(Bawin et al, 1975(Bawin et al, , 1978Blackman et al, 1980Blackman et al, , 1989Dutta et al, 1984;Goodman et al, 1995;Kwee and Raskmark, 1998;Lin-Liu and Adey, 1982;Penafiel et al, 1997;Velizarov et al, 1999;Xenos and Margas, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effect of GSM 900-MHz Mobile Phone Radiation on the Reproductive Capacity ofDrosophila melanogaster

Panagopoulos

Karabarbounis

Margaritis

2004

Electromagnetic Biology and Medicine

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Pulsed radio frequency, (RF), electromagnetic radiation from common GSM mobile phones, (Global System for Mobile Telecommunications) with a carrier frequency at 900 MHz, ''modulated'' by human voice, (speaking emission) decreases the reproductive capacity of the insect Drosophila melanogaster by 50%-60%, whereas the corresponding ''nonmodulated'' field (nonspeaking emission) decreases the reproductive capacity by 15%-20%. The insects were exposed to the near field of the mobile phone antenna for 6 min per day during the first 2-5 days of their adult lives. The GSM field is found to affect both females and males. Our results suggest that this field-radiation decreases the rate of cellular processes during gonad development in insects.

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Changes in cell proliferation due to environmental non-ionizing radiation

Cited by 73 publications

References 23 publications

Cytogenetic damage in human lymphocytes following GMSK phase modulated microwave exposure

Cytogenetic damage in human lymphocytes following GMSK phase modulated microwave exposure

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Effect of GSM 900-MHz Mobile Phone Radiation on the Reproductive Capacity ofDrosophila melanogaster

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