Changes in cell membrane microviscosity associated with adsorption of viruses Differences between fusing and non‐fusing viruses

Levanon, Avigdor; Kohn, Alexander

doi:10.1016/0014-5793(78)80465-7

Cited by 20 publications

(4 citation statements)

References 16 publications

(6 reference statements)

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“…I shall not review virus-erythrocyte fusion as these cells do not normally support replication (but for exceptions see Dimmock et al, 1981) and have been thoroughly reviewed by others (see Howe & Lee, 1972;B~ichi et al, 1977;Knutton, 1978). I refer readers to Levanon & Kohn (1978) and Kohn (1979) for a discussion of the effects of virus on the fluidity of cellular membranes. Paramyxoviruses are the fusers par excellence, and fusion has been demonstrated not only using cell lines but also with ciliated cells from the respiratory tract (Dourmashkin & Tyrrell, 1970) …”

Section: Fusionmentioning

confidence: 99%

Initial Stages in Infection with Animal Viruses

Dimmock

1982

Journal of General Virology

171

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Section: Fusionmentioning

confidence: 99%

Initial Stages in Infection with Animal Viruses

Dimmock

1982

Journal of General Virology

171

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“…It is possible that virion capsid proteins could bind to cell membrane receptors and induce changes in membrane permeability to PAP. Paramyxovirus infection has been shown to cause changes in membrane integrity including leakage of ions (16), modification of the resting membrane potential (8), and increased fluidity of the cell membrane (12). Thus, changes in membrane integrity could allow PAP to enter virus-infected cells.…”

mentioning

confidence: 99%

Poliovirus-mediated entry of pokeweed antiviral protein

Lee

Crowell

Shearer

et al. 1990

Antimicrob Agents Chemother

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“…The mean fluorescence lifetime of dPGS-ICC determined in HeLa cell membranes of τ ̅ pop = 1.37 ns correlates to an apparent nanoviscosity of 187 cP, according to the viscosity calibration curves in Figure 3f. This value is in the viscosity range of SK-OV3 cell membranes determined with 20−450 cP 19 and of HeLa cell membrane viscosities measured with various fluorescence techniques yielding values between 155 69 and 331 cP. 70 The determined apparent cell membrane viscosity of HeLa cells rather falls in the lower viscosity range of the HeLa cell membrane values from the literature.…”

Section: ■ Results and Discussionmentioning

confidence: 70%

Expanding the Scope of Reporting Nanoparticles: Sensing of Lipid Phase Transitions and Nanoviscosities in Lipid Membranes

et al. 2019

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Biological membrane fluidity and thus the local viscosity in lipid membranes are of vital importance for many life processes and implicated in various diseases. Here, we introduce a novel viscosity sensor design for lipid membranes based on a reporting nanoparticle, a sulfated dendritic polyglycerol (dPGS), conjugated to a fluorescent molecular rotor, indocarbocyanine (ICC). We show that dPGS-ICC provides high affinity to lipid bilayers, enabling viscosity sensing in the lipid tail region. The systematic characterization of viscosity-and temperature-dependent photoisomerization properties of ICC and dPGS-ICC allowed us to determine membrane viscosities in different model systems and in living cells using fluorescence lifetime imaging (FLIM). dPGS-ICC distinguishes between ordered lipids and the onset of membrane defects in small unilamellar single lipid vesicles and is highly sensitive in the fluid phase to small changes in viscosity introduced by cholesterol. In microscopy-based viscosity measurements of large multilamellar vesicles, we observed an order of magnitude more viscous environments by dPGS-ICC, lending support to the hypothesis of heterogeneous nanoviscosity environments even in single lipid bilayers. The existence of such complex viscosity structures could explain the large variation in the apparent membrane viscosity values found in the literature, depending on technique and probe, both for model membranes and live cells. In HeLa cells, a tumor-derived cell line, our nanoparticle-based viscosity sensor detects a membrane viscosity of ∼190 cP and is able to discriminate between cell membrane and intracellular vesicle localization. Thus, our results show the versatility of the dPGS-ICC nano-conjugate in physicochemical and biomedical applications by adding a new analytical functionality to its medical properties.

show abstract

Changes in cell membrane microviscosity associated with adsorption of viruses Differences between fusing and non‐fusing viruses

Cited by 20 publications

References 16 publications

Initial Stages in Infection with Animal Viruses

Initial Stages in Infection with Animal Viruses

Poliovirus-mediated entry of pokeweed antiviral protein

Expanding the Scope of Reporting Nanoparticles: Sensing of Lipid Phase Transitions and Nanoviscosities in Lipid Membranes

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