“…However, due to the rapid growth of poplar, its short renewal cycle, and its high water consumption-coupled with the cultivation using a single method, the use of artificial forests; shelterbelts mostly being built in desert sand and hilly areas, poor site conditions, and low soil fertility-a decline in poplar plantation quality has occurred, and the special results showed that the growth of poplar decreased, the soil of forest land degraded, and the biodiversity weakened [42][43][44]. To date, studies on the effects of nutrient elements on poplar growth have mainly focused on single elements, such as available potassium [45,46], alkali-hydrolyzed nitrogen [47,48], soluble calcium [49], available phosphorus [46,50], and C:N:P stoichiometry [51]. However, there is a lack of attention to the value of the N:Ca ratio and its transcriptome.…”