Cerebral metabonomics study on Parkinson's disease mice treated with extract of Acanthopanax senticosus harms

Neuroprotection or neurotoxicity? new insights into the effects of Acanthopanax senticosus harms on nervous system through cerebral metabolomics analysis

“…The voucher specimen (hlj-201003) of the herb was authenticated by Professor Ke Fu, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine. The preparation of extracts was shown in our pre-studies (Li et al, 2013b;Li et al, 2014;Liu et al, 2012). Briefly, the crude drugs were extracted 3 times with 10 volume of 80% ethanol for 2 h each and purified by AB-8 macroporous adsorption resin.…”

“…Male Sprague-Dawley rats (1 year old, $250 g) were obtained from Drug Safety Evaluation Center (Heilongjiang University of Chinese Medicine) and randomly divided into control and AS-treated groups (10 each). According to the State Pharmacopoeia of PR China and our own recent studies, the average daily dose of AS per rat was 31.6 mg/kg body weight (ChPC, 2010;Li et al, 2013b;Liu et al, 2012). AS-treated group was orally administrated with AS once a day for 20 days, and control group received equal volume saline once a day for 20 days.…”

“…Any medicine should be used for the treatment of diseases on the premise of safety or within the controllable range of toxicity. The change in the expression levels of endogenous metabolites can be used to characterize the therapeutic effects of herbal medicine (Li et al, 2013b) and assess their toxicity (Lu et al, 2013). Emerging metabolomics provides a powerful platform for monitoring multiple endogenous metabolites levels simultaneously in the samples from cell lines (Gao et al, 2014), tissues (Li et al, 2013b), and body fluids (Lu et al, 2013;Zhao et al, 2012).…”

“…The change in the expression levels of endogenous metabolites can be used to characterize the therapeutic effects of herbal medicine (Li et al, 2013b) and assess their toxicity (Lu et al, 2013). Emerging metabolomics provides a powerful platform for monitoring multiple endogenous metabolites levels simultaneously in the samples from cell lines (Gao et al, 2014), tissues (Li et al, 2013b), and body fluids (Lu et al, 2013;Zhao et al, 2012). Metabolomics, as one of the major components of systems biology, is defined as 'the quantitative measurement of the dynamic multi-parametric metabolic response of living systems to pathophysiological stimuli or genetic modification' (Nicholson et al, 1999).…”

The Potential Targets of Chinese Medicines in the Treatment of Parkinson’s Disease: Old Wine in New Bottles

“…The therapeutic effect of EAS on PD may involve in regulating the tyrosine metabolism, mitochondrial beta-oxidation of long chain saturated fatty acids, fatty acid metabolism, methionine metabolism and sphingolipid metabolism. This study indicated that changed metabolites can be certainly recovered by EAS, and the treatment of EAS can be connected with the regulation of related metabolic pathways [12,39].…”