Cerebral bioimaging of Cu, Fe, Zn, and Mn in the MPTP mouse model of Parkinson’s disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)

Matusch, Andreas; Depboylu, Candan; Palm, Christoph; Wu, Bei; Höglinger, Günter U.; Schäfer, Martin; Becker, J. Sabine

doi:10.1016/j.jasms.2009.09.022

Cited by 184 publications

(133 citation statements)

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“…Following rehydration, the tissue was analyzed using a Bruker Autoflex speed tandem time-of-flight (TOF/TOF) mass spectrometer (Bruker Daltonics, Billerica, MA) equipped with a Gaussian beam profile laser (Nd:YAG; 355 nm) in linear positive-ion mode at 75-m spatial resolu- 25 Mg, 31 P, 44 Ca, 55 Mn, 56 Fe, 63 Cu, 66 Zn Runs (no.) 10 Passes (no.)…”

Section: Methodsmentioning

confidence: 99%

“…Slide-mounted slices of kidney tissue were placed in a sealed ablation cell and ablated in multiline mode (line by line) with a focused Nd:YAG laser beam that operated at a quadrupled wavelength of 213 nm, energy of 50%, laser shot frequency of 20 Hz, and laser spot size of 100 m. Helium gas was used as the cell gas during ablation for better sample transport efficiency. The ablated sample particles were transported by He from the ablation cell and then mixed with argon gas before online introduction into the ICP-MS for element intensity detection of the following ions: 44 Ca ϩ , 55 Mn ϩ , and 66 Zn ϩ . The operational parameters for the laser ablation system and ICP-MS are listed in Table 3.…”

Section: Methodsmentioning

confidence: 99%

“…Although the kidneys appear to be Mn limited, the similar bacterial burdens in the kidneys of wild-type and CP-deficient mice suggest that CP does not contribute substantially to Mn sequestration in this organ (14). To address this possibility, IMS was employed to image metal and protein levels in infected organs (14,(61)(62)(63)(64)(65)(66). Imaging LA-ICP-MS was used to examine metal distribution in the kidneys of wild-type C57BL/6 and CP-deficient mice infected with S. aureus.…”

Section: Fig 4 Mntabc and Mnth Are Necessary For Invasive S Aureus Dmentioning

confidence: 99%

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MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

et al. 2013

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During infection, vertebrates limit access to manganese and zinc, starving invading pathogens, such as Staphylococcus aureus, of these essential metals in a process termed "nutritional immunity." The manganese and zinc binding protein calprotectin is a key component of the nutrient-withholding response, and mice lacking this protein do not sequester manganese from S. aureus liver abscesses. One potential mechanism utilized by S. aureus to minimize host-imposed manganese and zinc starvation is the expression of the metal transporters MntABC and MntH. We performed transcriptional analyses of both mntA and mntH, which revealed increased expression of both systems in response to calprotectin treatment. MntABC and MntH compete with calprotectin for manganese, which enables S. aureus growth and retention of manganese-dependent superoxide dismutase activity. Loss of MntABC and MntH results in reduced staphylococcal burdens in the livers of wild-type but not calprotectin-deficient mice, suggesting that these systems promote manganese acquisition during infection. During the course of these studies, we observed that metal content and the importance of calprotectin varies between murine organs, and infection leads to profound changes in the anatomical distribution of manganese and zinc. In total, these studies provide insight into the mechanisms utilized by bacteria to evade host-imposed nutrient metal starvation and the critical importance of restricting manganese availability during infection. Staphylococcus aureus is a commensal organism that asymptomatically colonizes nearly one-third of the population (1). However, once S. aureus breaches the epithelial barrier, the bacterium is capable of infecting nearly every organ despite the robust defenses elaborated by the host (2). This adaptability contributes to the significant morbidity and mortality associated with S. aureus infections. The emergence of methicillin-and vancomycin-resistant isolates has compounded the threat of this organism, highlighting the need to identify new therapeutics (3-7). This is of particular importance, as antibiotic resistance is prevalent in both hospital-and community-acquired isolates (4,6,8).Metals are essential for all forms of life due to their critical contributions to protein structure and enzymatic function (9-12). To combat invading pathogens, vertebrates leverage the essentiality of transition metals by restricting their availability, a process termed "nutritional immunity" (10, 13). While the most prominent example of nutritional immunity is the restriction of iron (Fe) by the host, it has recently been discovered that vertebrates also limit manganese (Mn) and zinc (Zn) availability during infection (10,(13)(14)(15)(16)(17). In fact, examination of abscesses formed during S. aureus infection has revealed that these lesions are Mn and Zn depleted (14). It was subsequently determined that the Mnand Zn-binding S100 protein calprotectin (CP) is a critical component of this nutrient-withholding response (10,14,18,19). CPdeficient mice...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Fig 4 Mntabc and Mnth Are Necessary For Invasive S Aureus Dmentioning

confidence: 99%

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MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

et al. 2013

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“…For example this technique has been used to investigate the etiology and pathogenesis of disease states arising from imbalances in metal homeostasis. The links between imbalances of transition metals, such as Fe, Zn, Cu and Mn, have been investigated in brain tissue as markers for Alzheimer's disease [15][16][17] and Parkinson's disease 18,19 , in retinal tissue as a factor in Age related Macular Degeneration 20 and in liver to study Wilson's disease 21 . To differentiate regions of interest, high-spatial resolution is required on the micron scale.…”

Section: Introductionmentioning

confidence: 99%

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

et al. 2015

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In recent years, laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) has gained increasing importance for biological analysis, where ultratrace imaging at micrometer resolution is required. However, while undoubtedly a valuable research tool, the washout times and sensitivity of current technology have restricted its routine and clinical application. Long periods between sampling points are required to maintain adequate spatial resolution. Additionally, temporal signal dispersion reduces the signal-to-noise ratio, which is a particular concern when analyzing discrete samples, such as individual particles or cells. This paper describes a novel, two-volume laser ablation cell and integrated ICP torch designed to minimize aerosol dispersion for fast, efficient sample transport. The holistic design utilizes a short, continuous diameter fused silica conduit, which extends from the point of ablation, through the ICP torch, and into the base of the plasma. This arrangement removes the requirement for a dispersive component for argon addition, and helps to keep the sample on axis with the ICP cone orifice. Hence, deposition of sample on the cones is theoretically reduced with a resulting improvement in the absolute sensitivity (counts per unit mole). The system described here achieved washouts of 1.5, 3.2, and 4.9 ms for NIST 612 glass, at full width half, 10%, and 1% maximum, respectively, with an 8–14-fold improvement in absolute sensitivity, compared to a single volume ablation cell. To illustrate the benefits of this performance, the system was applied to a contemporary bioanalytical challenge, specifically the analysis of individual biological cells, demonstrating similar improvements in performance.

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“…17 Examples include the analysis of copper and zinc in liver and kidney sections, 18,19 the mapping of copper, zinc, iron and manganese in sections of brain tissue, [20][21][22][23][24] and the quantification of metal binding proteins in gels following electrophoretic separation of blood serum. 25 Recently, the technique has been applied to image the distribution of iodine, silver and gold in single fibroblast cells, following labelling in vitro with KI 3 and gold and silver nanoparticles respectively.…”

Section: Introductionmentioning

confidence: 99%

Single Cell Tracking of Gadolinium Labeled CD4⁺ T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

et al. 2013

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Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of hematopoietic stem cell (HSC) transplantation, autoimmune disease, and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labeling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4 + T cells were labeled with commercially available Gd-based magnetic resonance imaging (MRI) contrast agents, Omniscan and Dotarem, which enabled passive loading of up to 10 8 Gd atoms per cell. In mixed preparations of labeled and unlabeled cells, LA-ICP-MS was capable of enumerating labeled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained a sufficient label to remain detectable for up to 10 days post-labeling both in vitro and in vivo in an immunodeficient mouse model.

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Cerebral bioimaging of Cu, Fe, Zn, and Mn in the MPTP mouse model of Parkinson’s disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)

Cited by 184 publications

References 50 publications

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

Single Cell Tracking of Gadolinium Labeled CD4⁺ T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

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Cerebral bioimaging of Cu, Fe, Zn, and Mn in the MPTP mouse model of Parkinson’s disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)

Cited by 184 publications

References 50 publications

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

Single Cell Tracking of Gadolinium Labeled CD4+ T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Contact Info

Product

Resources

About

Single Cell Tracking of Gadolinium Labeled CD4⁺ T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry