Cereal microspore culture

Jähne, A.; Lörz, Horst

doi:10.1016/0168-9452(95)04149-o

Cited by 133 publications

(56 citation statements)

References 71 publications

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“…There are many advantages of microspore culture over anther culture for producing haploid plants: space to grow and access to nutrition is not limited; there is not somatic tissue contamination; and microspore development is easier to follow visually. The mode of regeneration of plantlets from isolated microspores tends to be more via an embryogenic route rather than via a callus phase (Jähne and Lörz 1995). Therefore, the genetic stability of microspore-derived plants should be higher.…”

Section: Mots Clésmentioning

confidence: 99%

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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. 1997. Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.). Can. J. Plant Sci. 77: 549-554. Ploidy level, genetic stability and field performance of isolated microspore-derived wheat plants were evaluated. The ploidy levels of isolated microspore-derived wheat plants from cv. Chris and reciprocal crosses of Chris × Sinton were determined by two methods, namely chromosome counts of root tip cells and flow cytometric measurement of nuclear DNA content from leaves. Both methods gave similar results with the frequencies of spontaneous chromosome doubling and completely fertile plants among microspore-derived H 0 plants of wheat being about 80% and 75%, respectively, based on two H 0 populations. Only 1.7% were aneuploid and 16% were haploid. Spontaneous mutation frequencies were low with 1 of 124 Chris DH plants having a recessive mutant for lemma awns while three were grass-like plants in the H 0 generation. The field-measured traits of microspore-derived DH lines were similar to the check by the third generation. Ninety-two percent and 70% of Chris DH lines had 1000-kernel weights and yields similar to the check, respectively. These results indicate that microspore-derived DH lines should have good potential for applications in plant breeding, in vitro selection, plant transformation and genetic studies.

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Section: Mots Clésmentioning

confidence: 99%

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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“…In the IMC large numbers e.g. 6 × 10 6 of haploid cells capable of synchronous development can be cultured in a small volume (1 ml) of medium (Jähne & Lörz 1995;Oleszczuk et al 2006). The relevant literature lacks a definite answer which of these two methods yields better results not only in barley (Li & Devaux 2003;Lantos et al 2014).…”

mentioning

confidence: 99%

The effect of copper on plant regeneration in barley microspore culture

Makowska¹,

Oleszczuk²,

Zimny³

2017

CZECH J GENET PLANT

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Makowska K., Oleszczuk S., Zimny J. (2017): The effect of copper on plant regeneration in barley microspore culture. Czech J. Genet. Plant Breed., 53: 17-22.Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture.

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“…Microspore culture efficiency in cereals is influenced by the microspore isolation methods from anthers or whole spikes. Based on previously published literature, we may distinguish four techniques to obtain microspores of cereals -cultures of spontaneously released microspores, microspores released by shaking, by maceration, or with the use of a blender (Jähne and Lörz, 1995;Gustafson et al, 1995).…”

Section: Resultsmentioning

confidence: 99%

“…(Shariatpanahi et al, 2006). In order to change the microspore development pathway into sporophytic, spike pre-treatment at 4EC is generally applied in microspore cultures of cereals (Jähne and Lörz, 1995).…”

Section: Resultsmentioning

confidence: 99%

The effect of cold temperature stress on the viability of rye ( Secale cereale L.) microspores

Mikołajczyk

Broda²,

Weigt³

2012

bta

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Rye has been generally considered to be recalcitrant to the androgenesis induction in vitro. The haploids of Secale cereale are not easily obtainable and the isolated microspore technique application is difficult, thus is performed occasionally, and in a limited number of strains and cultivars. The aim of the conducted experiment was to assess the frequency of mechanical damage in microspores after their isolation in the mortar, and the effect of the pretreatment of spikes at 4EC on microspore viability and the course of the culture. The analyses in the isolated microspore culture were conducted on 30 genotypes of rye from two breeding programs and differing in their origin. Spikes with microspores in the unicellular stage were cut and exposed to a temperature of 4EC for a period of 2 to 42 days. Microspore observations were conducted immediately after the microspore isolation in order to assess the viability and percentage of the damaged ones. In order to study the mitotic divisions first, cultures of rye microspores were examined after 3 days. The highest microspore viability after isolation was observed for strain 1283C -91.9% and inbred line S1152/10 -91.8%, while it was the lowest for F 1 hybrid S02779/10 -79.8% and strain 1252E -84%, respectively. It was found that to maintain high (microspore -to mozna opuscic) viability, microspores must be isolated after low temperature stress (+4EC) applied for up to 7 days of culture. A considerable extension of the duration of low temperature (36-42 days) resulted in a reduction of the microspore isolation efficiency in rye (a lower number of microspores in the obtained suspension) and a slight reduction of microspore viability.

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Cereal microspore culture

Cited by 133 publications

References 71 publications

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

The effect of copper on plant regeneration in barley microspore culture

The effect of cold temperature stress on the viability of rye ( Secale cereale L.) microspores

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