Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis

Wirawan, Ferdian; Cheng, Chung-Wei; Kao, Wei Chen; Lee, Dong Hoon; Chang, Jo Shu

doi:10.1016/j.apenergy.2012.04.032

Cited by 69 publications

(30 citation statements)

References 29 publications

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“…The flasks were incubated statically at 30 C and samples were withdrawn at the indicated time intervals and centrifuged at 10,000 £ g for 10 min, filtered through 0.45 mm membranes and used for analysis of glucose, ethanol, furfural and HMF concentration by HPLC. 39 Pellet was used for trehalose extraction as mentioned above. The HPLC system was equipped with a refractive index detector and ICSep ICE-COREGEL 87H3 column (Transgenomic, Omaha, USA).…”

Section: Ethanol Production Capacity Under Stress Conditionsmentioning

confidence: 99%

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

et al. 2017

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Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S. cerevisiae. The engineered strain exhibited ethanol tolerance up to 14% of ethanol, while the growth of wild strain was inhibited by 6% of ethanol. Compared with the wild strain, the engineered strain showed greater ethanol yield under high stress condition induced by combining 30% glucose, 30 mM furfural and 30 mM 5-hydroxymethylfurfural (HMF).

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Section: Ethanol Production Capacity Under Stress Conditionsmentioning

confidence: 99%

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

et al. 2017

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“…Compilation of ethanol fermentation from sugar feedstock suggests that PVAalginate entrapment is an efficient method to prepare biocatalysts for ethanol fermentation (Table 6). This could be associated with its unique biophysical properties, such as remarkable stability, high mechanical strength, and durability (Takei et al, 2011;Wirawan et al, 2012). ).…”

Section: Discussionmentioning

confidence: 99%

“…IPNs are defined as a blend of 2 or more polymers in a network form, at least 1 of which is synthesized and/or cross-linked in the immediate presence of the other(s) (Sperling and Hu, 2003). Among several types of IPNs, synthetic polyvinyl alcohol (PVA) is recognized as a promising carrier for immobilization because it is cheap, is nontoxic for microbes, and has high mechanical strength and durability (Wirawan et al, 2012;Wang et al, 2013). The simplest procedure to immobilize microbial cells using PVA is the PVA-boric acid method.…”

Section: Introductionmentioning

confidence: 99%

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Nonthasen¹,

Piyatheerawong²,

Thanonkeo³

2015

Turk J Biol

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The aim of this investigation was to optimize immobilization conditions to entrap Kluyveromyces marxianus DBKKUY-103 during ethanol fermentation from sweet sorghum juice using a response surface methodology. The effects and interactions of variables involved in cell entrapment using polyvinyl alcohol and sodium alginate were studied through a fractional factorial design. The results suggested that the primary variables involved in cell entrapment that significantly affected ethanol fermentation were sodium sulfate concentration, polyvinyl alcohol, and sodium alginate contents (P < 0.01). Subsequently, these variables were optimized for the preparation of cell entrapment based on response surface methodology and using central composite design. The results indicate that cell entrapment can achieve an ethanol concentration of 81.56 g L -1 under the following optimal conditions: bead gel diameter of 4.0 mm, calcium chloride concentration of 0.3 M, polyvinyl alcohol content of 10.09% (w v -1 ), sodium alginate content of 3.39% (w v -1 ), and sodium sulfate concentration of 0.44 M. Furthermore, statistical analysis of the model reveals that it adequately fit the experimental data (P < 0.01, R 2 = 0.9879). Therefore, this model can be used to efficiently prepare entrapped cells for ethanol production.

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“…SSF has been extensively explored for cellulosic ethanol production (BIAŁAS; SZYMANOWSKA; CANTARELLA et al, 2004;DAS NEVES et al, 2006;SANTOS et al, 2012b;WIRAWAN et al, 2012). The major advantage with SSF over SHF is the reduction of the process time by combining hydrolysis and fermentation (OLOFSSON et al, 2008).…”

Section: Fermentation Of the Sugars Recovered From Lignocellulosic Bimentioning

confidence: 99%

“…SSF uses either suspended or immobilized microorganisms (WIRAWAN et al, 2012) or free enzymes in the saccharification medium (SANTOS et al, 2012b). Operation costs can be reduced by recycling the enzymes or by recycling the solid fraction after enzymatic hydrolysis (VISSER et al, 2015).…”

Section: Wallberg 2014) Hexose Fermentation Is a Well-known Processmentioning

confidence: 99%

Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

Gonçalves¹

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Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis

Cited by 69 publications

References 29 publications

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

Efficient entrapment of Kluyveromyces marxianus DBKKUY-103 in polyvinyl alcohol hydrogel for ethanol production from sweet sorghum juice

Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

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