Cellulases in paper and pulp, brewing and food industries: Principles associated with its diverse applications

Basak, Piyali; Adhikary, Tathagata; Das, Pratik; Shee, Moumita; Dutta, Tanusree; Biswas, Soumyajit; Paul, Samrat; Manna, Suvendu

doi:10.1016/b978-0-12-821882-2.00003-x

Cited by 3 publications

(3 citation statements)

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“…pastoris culture cultivation was carried out both in shake-flasks and in bioreactors, according to Invitrogen Pichia Fermentation Process Guidelines and in-house methods. P. pastoris is a facultative anaerobe which, despite recombinant expression significantly increasing upon oxygen deprivation (Baumann et al, 2011), requires some oxygen dispersion in growth media. Therefore, aeration was done in all cultivation instances.…”

Section: Cell Cultivationmentioning

confidence: 99%

“…Taking advantage of enzymes such as carbohydrate-active enzymes (CAZys) is at the foundation of paper and pulp industry, which make paper, carton, tissues and paper/pulp by-products possible (Basak et al, 2021). Utilizing cellulase mixtures is the most commonly chosen strategy when high substrate specificity and hydrolytic potential is of essence (Hu et al, 2013).…”

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confidence: 99%

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Structure and function studies of GH45 glycoside hydrolases

Okmane¹

2023

Acta Universitatis Agriculturae Sueciae

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The enzymatic hydrolysis of lignocellulosic material in nature is carried out by a plethora of cellulases. Glycoside hydrolase family 45 (GH45) enzymes are small cellulases most commonly found in fungi that catalyse the hydrolysis of β(1→4) linked glucans. Additionally, GH45 enzymes display a structural resemblance to non-hydrolytic protein groups such as expansins and loosenins. In this thesis, the distinctness of GH45 enzymes from different subfamilies was explored. The enzymatic activity of GH45 enzymes from Humicola insolens (HiCel45A), Mytilus edulis (MeCel45A), Trichoderma reesei (TrCel45A), Phanerochaete chrysosporium (PcCel45A), Gloeophyllum trabeum (GtCel45A) was demonstrated. Among the tested substrates were barley betaglucan, konjac glucomannan, carboxymethyl cellulose, and cellohexaose. Initial hydrolysis rates and hydrolysis yields were determined by reducing sugar assays, product formation was analysed using NMR spectroscopy and HPLC. The subfamily B and C enzymes exhibited mannanase activity, and the subfamily B enzyme MeCel45A appeared cold adapted in comparison to TrCel45A. GH45 enzymes are known to act using an inverting action mechanism. This action mechanism had not been experimentally demonstrated in subfamilies B and C, however. Here experimental evidence is provided for the inverting nature of GH45 enzymes from all subfamilies. Comparisons of GH45 enzyme structures were carried out and the first crystal structure of a GH45 subfamily C enzyme from the brown-rot fungus G. trabeum was reported at 1.3 Å resolution. Furthermore, structure and function investigations were done on an isotopically labelled Cel45A from the white-rot fungus P. chrysosporium using NMR spectroscopy. PcCel45A was expressed in Pichia pastoris with 13C and 15N labelling. A nearly complete assignment of 1 H, 13C and 15N backbone resonances was obtained and the interaction of 15Nlabelled PcCel45A with cellobiose was studied.

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Section: Cell Cultivationmentioning

confidence: 99%

mentioning

confidence: 99%

Structure and function studies of GH45 glycoside hydrolases

Okmane¹

2023

Acta Universitatis Agriculturae Sueciae

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“…Several bacterial strains have been isolated and selected and their physiology, genetics and biochemistry for the production of biomolecules explored (Chadha, 2019). India appears to be one of the hubs of manufacturing units that use microbial enzymes that have been isolated and characterized by native microbiologists with promising applicability in the textile industry to improve the quality of the fabrics; in the pulp and paper industry in biomechanical pulping and bleaching; and in the food industry for the fermentation processes for the production of bread and drinks such as wine and beer (Basak et al ., 2021; Tamang, 2021). Indian microbiologists have also developed probiotics and fermented foods by using ‘ethno‐microbiology’ (which relates to the study of indigenous Indian knowledge to produce culturally and organoleptically acceptable fermented foods through natural fermentation).…”

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confidence: 99%