Cellulases by Penicillium sp. in different culture conditions

Abstract

The high cost of cellulolytic enzymes used in the ethanol production process has led to a growing interest in situ production. The evaluation of the influence of several factors in the fungus Penicillium sp. cellulase production using pretreated sugarcane bagasse is very interesting. Penicillium sp. cellulase production by using filter paper as cellulosic substrate and the use of glucose, sucrose and lactose like co-substrates was assessed. In the experiments using filter paper as a cellulosic substrate, the highest FPase enzyme activity obtained was 280 FPU.L-1 using sucrose as co-substrate. Subsequently, the study of pretreated sugarcane bagasse was conducted using Plackett-Burman experimental design with analysis of 6 factors influencing the process. The highest FPase activity was 615.1 FPU.L-1. The factors influencing FPase and β- glucosidase activity were the use of molasses and the solid loading. The successful use of molasses as co-substrate opens perspectives for future researches.

Recycled of commercial enzymes in the production of ethanol of II generation

“…This first analysis was carried out under the conditions described in Mesa (2010) [8] for the case of the application of two pretreatment stages to sugarcane bagasse and the configuration of enzymatic hydrolysis and fermentation separately. Enzymatic hydrolysis was carried out, as reported by Mesa et al (2016) [6] with an enzymatic load of cellulase of 10 FPU / g of pretreated substrate in dry base, 2.5% of surfactant based on dry fiber and 10% solids in the enzymatic hydrolysis. The enzymatic hydrolysis was carried out for 24 hours as well as the alcoholic fermentation.…”

“…Some of these byproducts present a great challenge for the chemical industry because they can be the raw material not only of ethanol but of several biodegradable compounds. Recirculation is a potential alternative to reduce the cost of enzymes, using their relatively high stability and high affinity for cellulose [6].The main difficulties to enzymatically hydrolyze lignocellulosic materials are related, on one hand, with the low specific activity of the enzymes currently available, and therefore with the need for a high consumption of them during the process, [7]. Among the advantages, it is known that enzymes are not consumed in the reactions that they catalyze; therefore, they are potentially recyclable.…”

“…However, there is limited efficiency in the recovery of enzymes after hydrolysis. The proposed procedure is based on the need for the systematic evaluation of the impact strategy of the recirculation of enzymes in the process of obtaining ethanol from bagasse reported by Mesa et al, (2016) [6]. Based on the criterion that all technology has to be economically feasible, it is necessary to complement the technological analysis with the economic ones.…”

Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

“…and for each type of sugarcane bagasse (natural and pre-treated with acid-alkaline and hydrogen peroxide solutions, as described in Salomão et al (2019)). The values of concentration and temperatures selected here were based on works found in the literature, which used agro-industrial residues to produce cellulases (Akinyele et al, 2014;Mesa et al, 2016;Jasani et al, 2016;Irfan et al, 2017;Fernandes et al, 2018). For these experiments, a 3 2 factorial design with two central points was used.…”

“…With regard to Penicillium, it is possible to find very different values of cellulase production in the scientific literature, which depends on the species, culture medium and physical conditions used. Mesa et al (2016), in a study of culture medium optimization, obtained a production of 13 IU/L (CMCase) by a wild strain of Penicillium spp., with the addition of 1.5 g/L of sugarcane bagasse pretreated with acid-alkaline solution. Santa-Rosa et al (2018) found an activity of 600 IU/L by a strain of Penicillium spp.…”