Cellulase Production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae Cultured on Plantain Peel

Omojasola, P. F.; Jilani, O. P.

doi:10.3923/jm.2009.67.74

Cited by 19 publications

(15 citation statements)

References 10 publications

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“…Fadel (2000) reported the maximum enzyme activity (216.2 IU/g) at an inoculum size of 10% with wheat straw as the substrate. Omojasola and Jilani (2009) reported the maximum cellulase activity with an inoculum size of 8%. …”

Section: Optimization Of Inoculum Sizementioning

confidence: 99%

The Industrial Applicability of Purified Cellulase Complex Indigenously Produced by Trichoderma viride Through Solid-State Bio-processing of Agro-Industrial and Municipal Paper Wastes

et al. 2012

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An indigenous strain of Trichoderma viride produced high titers of cellulase complex in solid-state bio-processing of agro-industrial orange peel waste, which was used as the growth-supporting substrate. When the conditions of the SSF medium containing 15 g orange peel (50% w/w moisture) inoculated with 5 mL of inoculum were optimal, the maximum productions of endoglucanase (655 ± 5.5 U/mL), exoglucanase (412 ± 4.3 U/mL), and β-glucosidase (515 ± 3.7 U/mL) were recorded after 4 days of incubation at pH 5 and 35 °C. The enzyme with maximum activity (endoglucanase) was purified by ammonium sulfate fractionation and Sephadex G-100 column gel filtration chromatographic technique. Endoglucanase was 5.5-fold purified with specific activity of 498 U/mg in comparison to the crude enzyme. The enzyme was shown to have a molecular weight of 58 kDa by sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE). The shelf life profile revealed that the enzyme could be stored at room temperature (30 °C) for up to 45 days without losing much of its activity.

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Section: Optimization Of Inoculum Sizementioning

confidence: 99%

The Industrial Applicability of Purified Cellulase Complex Indigenously Produced by Trichoderma viride Through Solid-State Bio-processing of Agro-Industrial and Municipal Paper Wastes

et al. 2012

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“…Fadel (2001) reported maximum cellulase activity by A. niger with 10% inoculum size using wheat straw as a substrate. Omojasola and Jilani (2009) reported 8% inoculum size suitable for maximum cellulase production by A. niger. Murad and Azzaz (2013) have reported 7% inoculum size optimum for maximum cellulase production by Aspergillus flavus using rice straw.…”

Section: Effect Of Inoculum Sizementioning

confidence: 99%

Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01

et al. 2017

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Alkali-assisted acid pretreated rice straw was saccharified using cellulase from Aspergillus niger BK01. The cellulase production by the fungus was enhanced by parametric optimization using solid-state fermentation conditions. Maximum cellulase production (12.0 U/gds of carboxymethyl cellulase, CMCase) was achieved in 96 h, using 6.0% substrate concentration, 7.5% inoculum concentration, 1:2 solid to liquid ratio, at pH 5.5, and temperature 28°C, by supplementation of the fermentation medium with 0.1% carboxymethylcellulose and 0.1% ammonium nitrate. Characterization of crude cellulases showed that highest CMCase activity was observed at pH 4.8 and temperature 40°C. The CMCase was stable from pH 4.8-5.5 and at a temperature range of 35-50°C. The pretreated biomass was subjected to hydrolysis with the fungal cellulases. The saccharification optimization studies showed that 2% (v/v) enzyme concentration and hydrolysis time of 2.5 h were optimum for maximum yield, i.e, 23.78% sugars and 35.96% saccharification value.

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“…Inoculum size affects endoglucanase and enzyme activity; and Omojasola and Jilani (2009) found maximum activity with 8% inoculum size. The early lag phase was influenced by the size of the inoculum; a smaller inoculum size reduced the lag phase, while a larger size lowered the production of endoglucanase enzyme by increasing the moisture content by a substantial amount (Swelim et al 2010).…”

Section: Production Of Endoglucanasementioning

confidence: 97%

Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation

2016

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A robust process of purification, characterization, and application of endoglucanase from the agro-industrial waste was performed using solid state fermentation (SSF). Trichoderma harzianum as a micro-organism and wheat straw as a growth supportive substrate were used in SSF under pre-optimized conditions. The maximum activity of 480 ± 4.22 U/mL of endoglucanase was attained when a fermentation medium was inoculated using 10% inoculum size and 3% substrate concentration with pH = 5.5 at 35 °C for an optimized fermentation period. In comparison with crude extract, enzyme was 1.83-fold purified with a specific activity of 101.05 U/mg using Sephadex-G-100 column chromatography. Sodium dodecyl sulfate (SDS) poly-acrylamide gel electrophoresis revealed that the enzyme exhibited a low molecular weight of 43 kDa. The purified enzyme displayed maximum activity at pH = 6 and a temperature of 50 °C, respectively. The maximum activity (Vmax) of 156 U/mL and KM value of 63 µM were observed. Ethylenediaminetetraacetic acid (EDTA), SDS, and Hg 2+ inhibited enzyme activity, while Co 2+ and Mn 2+ enhanced enzyme activity at 1 mM concentration. The maximum substrate affinity and specific activity of biosynthesized endoglucanase revealed that it can be potentially useful for industrial applications.

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Cellulase Production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae Cultured on Plantain Peel

Cited by 19 publications

References 10 publications

The Industrial Applicability of Purified Cellulase Complex Indigenously Produced by Trichoderma viride Through Solid-State Bio-processing of Agro-Industrial and Municipal Paper Wastes

The Industrial Applicability of Purified Cellulase Complex Indigenously Produced by Trichoderma viride Through Solid-State Bio-processing of Agro-Industrial and Municipal Paper Wastes

Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01

Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation

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