Cellular uptake mechanism and intracellular fate of hydrophobically modified pullulan nanoparticles

Jiang, Liqin; Li, Xuemin; Liu, Lingrong; Zhang, Qiqing

doi:10.2147/ijn.s44342

Cited by 32 publications

(17 citation statements)

References 47 publications

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“…Grafting of hydrophobic segments onto the hydrophilic polymeric backbone leads to amphiphilic polymers which form self-associate thermodynamically stable nanogel structures, with an inner hydrophobic core. Stable in size over time, such polymeric micelles have been recognized as promising drug carriers, the hydrophobic core-shell structure being able to trap hydrophobic drugs ( Liu et al, 2008 ; Bataille et al, 2011 ; Jiang et al, 2013 ). In this respect, the most cited paper was an early work from 1993 on the self-assembly of cholesteryl-bearing pullulan (CHP), forming stable hydrogel nanoparticles, which represented the starting point for future perspective drug-release complexes ( Bataille et al, 2011 ).…”

Section: Potential Applicationsmentioning

confidence: 99%

Present and future medical applications of microbial exopolysaccharides

2015

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Microbial exopolysaccharides (EPS) have found outstanding medical applications since the mid-20th century, with the first clinical trials on dextran solutions as plasma expanders. Other EPS entered medicine firstly as conventional pharmaceutical excipients (e.g., xanthan – as suspension stabilizer, or pullulan – in capsules and oral care products). Polysaccharides, initially obtained from plant or animal sources, became easily available for a wide range of applications, especially when they were commercially produced by microbial fermentation. Alginates are used as anti-reflux, dental impressions, or as matrix for tablets. Hyaluronic acid and derivatives are used in surgery, arthritis treatment, or wound healing. Bacterial cellulose is applied in wound dressings or scaffolds for tissue engineering. The development of drug controlled-release systems and of micro- and nanoparticulated ones, has opened a new era of medical applications for biopolymers. EPS and their derivatives are well-suited potentially non-toxic, biodegradable drug carriers. Such systems concern rating and targeting of controlled release. Their large area of applications is explained by the available manifold series of derivatives, whose useful properties can be thereby controlled. From matrix inclusion to conjugates, different systems have been designed to solubilize, and to assure stable transport in the body, target accumulation and variable rate-release of a drug substance. From controlled drug delivery, EPS potential applications expanded to vaccine adjuvants and diagnostic imaging systems. Other potential applications are related to the bioactive (immunomodulator, antitumor, antiviral) characteristics of EPS. The numerous potential applications still wait to be developed into commercial pharmaceuticals and medical devices. Based on previous and recent results in important medical-pharmaceutical domains, one can undoubtedly state that EPS medical applications have a broad future ahead.

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Section: Potential Applicationsmentioning

confidence: 99%

Present and future medical applications of microbial exopolysaccharides

2015

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“…[35][36][37][38] Absorption peaks of FITC might be expected between 1400 -1500 cm -1 , corresponding to aromatic ring stretching vibrations. 39 However, the FITC signal is obscured by the protein signals, which are both strong and complex in this region. The FITC-OVA/PLGA particles exhibited the IR bands of both PLGA and OVA, including the ester functional groups of PLGA (1084, 1174, 1270, and 1752 cm -1 ) and the protein amide region (1534 and 1654 cm -1 ).…”

Section: Particle Characterizationmentioning

confidence: 99%

“…41 In vitro release studies were performed using the shaking method, commonly employed to study the release kinetics of microparticles. 26,[39][40] The cumulative release and concentration profiles of the FITC-OVA/PLGA particles are given in .70 % of the total release) was initially evident. This was followed by fluctuation of the protein concentration over the range from 2.5 ± 0.6 μg/mL (ca.…”

Section: Drug Loading and Releasementioning

confidence: 99%

A Novel Transdermal Protein Delivery Strategy via Electrohydrodynamic Coating of PLGA Microparticles onto Microneedles

Angkawinitwong

Courtenay

Rodgers

et al. 2020

ACS Appl. Mater. Interfaces

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Transdermal delivery of biological therapeutics is emerging as a potent alternative to intravenous or subcutaneous injections. The latter come with major challenges including patient discomfort, the necessity for trained personnel, specialized sharps disposal, and risk of infection. Microneedle (MN) technology circumvents many of the abovementioned challenges, delivering biological material directly into the skin and allowing sustained release of the active ingredient both in animal models and in humans. This study describes the use of electrohydrodynamic atomization (EHDA) to coat ovalbumin (OVA)-encapsulated PLGA nanoparticles onto hydrogel-forming MN arrays. The particles showed extended release of OVA over ca. 28 days. Microscopic analysis demonstrated that EHDA could generate a uniform particle coating on the MNs, with 30% coating efficiency. Furthermore, the coated MN array manifested similar mechanical characteristics and insertion properties to the uncoated system, suggesting the coating should have no detrimental effects on the application of the MNs. The coated MNs resulted in no significance increase in anti-OVA specific IgG titres in C57BL/6 mice in vivo as compared to the untreated mice (paired t-test, p >0.05) indicating that the formulations are non-immunogenic. The approach of using EHDA to coat a MN array thus appears to have potential as a novel non-invasive protein delivery strategy.

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“…116 The caveolae-mediated endocytosis pathway is characteristic by the evolution of caveolae-derivatives of the subdomains of sphingolipid and cholesterol-rich cell membrane fractions, which mediates the translocation to the Golgi apparatus, to endoplasmic reticulum or entered into the endosomal pathway. 117 It has been revealed that the pathway of nanoparticles into cells can be regulated by shape. For instance, Hao et al 105 used sphere-nanoparticles (NS), short-rod nanoparticles (NSR), and Their results preliminarily demonstrated that NS particles preferred to be internalized into cells via the clathrinmediated pathway, whereas the uptake is shifted to the caveolae-mediated pathway for the particles with large aspect ratios (ARs).…”

Section: Effects Of Nanoparticle Morphology On Cellular Uptake In Vivmentioning

confidence: 99%

Section: The Effect Of Shape For Oral Bioavailabilitymentioning

confidence: 99%

<p>The Influence of Nanoparticle Properties on Oral Bioavailability of Drugs</p>

Wang

Feng

et al. 2020

IJN

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Oral administration has been the most common therapeutic regimen in various diseases because of its high safety, convenience, lower costs, and high compliance of patients. However, susceptible in hostile gastrointestinal (GI) environment, many drugs show poor permeability across GI tract mucus and intestinal epithelium with poor oral absorption and limited therapeutic efficacy. In recent years, nanoparticulate drug delivery systems (NDDS) have become a hot research spot because of their unique advantages including protecting drug from premature degrading and interacting with the physiological environment, increasing intracellular penetration, and enhancing drug absorption. However, a slight change in physicochemistry of nanoparticles can significantly impact their interaction with biological pathways and alter the oral bioavailability of drugs. Hence, this review focuses on the factors affecting oral bioavailability from two aspects. On the one hand, the factors are the biochemical and physiological barriers in oral drugs delivery. On the other hand, the factors are the nanoparticle properties including size, surface properties, and shape of nanoparticles.

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Cellular uptake mechanism and intracellular fate of hydrophobically modified pullulan nanoparticles

Cited by 32 publications

References 47 publications

Present and future medical applications of microbial exopolysaccharides

Present and future medical applications of microbial exopolysaccharides

A Novel Transdermal Protein Delivery Strategy via Electrohydrodynamic Coating of PLGA Microparticles onto Microneedles

<p>The Influence of Nanoparticle Properties on Oral Bioavailability of Drugs</p>

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