Cellular Energetics: Actin and Myosin Abstain from ATP during Starvation

Buelto, Destiney; Duncan, Mara C.

doi:10.1016/j.cub.2014.09.004

Cited by 3 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, the model distinguishes between actin cortex components that support energy starvation and those that require energy consumption. Although actin polymerization is a major consumer of ATP, cells account for well-conserved mechanisms to stabilize the actin cytoskeleton in absence of ATP 45,49 . This is consistent with a quiescent state of the cell, in which the actin cortex adopts a minimal but stable conformation.…”

Section: Resultsmentioning

confidence: 99%

Effects of energy metabolism on the mechanical properties of breast cancer cells

et al. 2020

View full text Add to dashboard Cite

Tumorigenesis induces actin cortex remodeling, which makes cancerous cells softer. Cell deformability is largely determined by myosin-driven cortical tension and actin fiber architecture at the cell cortex. However, it is still unclear what the weight of each contribution is, and how these contributions change during cancer development. Moreover, little attention has been paid to the effect of energy metabolism on this phenomenon and its reprogramming in cancer. Here, we perform precise two-dimensional mechanical phenotyping based on power-law rheology to unveil the contributions of myosin II, actin fiber architecture and energy metabolism to the deformability of healthy (MCF-10A), noninvasive cancerous (MCF-7), and metastatic (MDA-MB-231) human breast epithelial cells. Contrary to the perception that the actin cortex is a passive structure that provides mechanical resistance to the cell, we find that this is only true when the actin cortex is activated by metabolic processes. The results show marked differences in the nature of the active processes that build up cell stiffness, namely that healthy cells use ATP-driven actin polymerization whereas metastatic cells use myosin II activity. Noninvasive cancerous cells exhibit an anomalous behavior, as their stiffness is not as affected by the lack of nutrients and ATP, suggesting that energy metabolism reprogramming is used to sustain active processes at the actin cortex.

show abstract

Section: Resultsmentioning

confidence: 99%

Effects of energy metabolism on the mechanical properties of breast cancer cells

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It has been reported that glucose starvation occurs during many diseases, such as hypoglycemia, heart attack, or highly proliferative cancers. 29 To investigate the ability of the nanoaptasensor to monitor ATP fluctuation at the subcellular level under glucose starvation, glucose was removed from the cell culture medium. Then, the nanoaptasensor was inserted into different regions of single cells to incubate for 2 min, and the electrochemical detections were performed in vivo.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…When the glucose starvation lasted for 40 min, the metabolic activity of cells within a dormant state maintains the viability of cells. 29 After ATP detection during the starvation process, the cell viability of penetrated cells was further verified by staining with Calcein-AM. It is surprisingly found that some of cells exhibit bright green fluorescence emissions in Figure 3B even when they underwent eight insertions (including four insertions into the nucleus and four insertions into the cytoplasm in the course of glucose starvation), indicating they are living.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…It has been reported that glucose starvation occurs during many diseases, such as hypoglycemia, heart attack, or highly proliferative cancers . To investigate the ability of the nanoaptasensor to monitor ATP fluctuation at the subcellular level under glucose starvation, glucose was removed from the cell culture medium.…”

Section: Resultsmentioning

confidence: 99%

“…The ATP fluctuation in the extracellular space has been a gradually decreasing trend in the first 40 min and may be ascribed to the ATP supply from the cytoplasm. When the glucose starvation lasted for 40 min, the metabolic activity of cells within a dormant state maintains the viability of cells . After ATP detection during the starvation process, the cell viability of penetrated cells was further verified by staining with Calcein-AM.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical Nanoaptasensor for Continuous Monitoring of ATP Fluctuation at Subcellular Level

Zheng

Wang

et al. 2020

Anal. Chem.

View full text Add to dashboard Cite

Monitoring the fluctuation of adenosine 5′-triphosphate (ATP) at the subcellular level is important for the study of cell energy metabolism. Herein, we fabricated an electrochemical nanoaptasensor for continuously monitoring ATP fluctuation at the subcellular level. A gold nanoelectrode with a diameter of 120 nm was fabricated, and ferrocene (Fc)-labeled anti-ATP aptamer was self-assembled onto the nanoelectrode surface to form a nanoaptasensor. In the presence of ATP, the ferrocene-labeled anti-ATP aptamer bound with two ATP units to form an ATP–aptamer conjugation, resulting in the close proximity of Fc to the nanoelectrode surface and then an increase of oxidation current of Fc. ATP can be detected with a detection limit of 26 μM within 2 min. Cell viability assays indicated that the nanoaptasensor was biocompatible with negligible biological effects. By taking advantage of the good biocompatibility of the nanoaptasensor, ATP fluctuation at the subcellular level was monitored under glucose starvation and Ca2+ induction. This work demonstrates that the nanoaptasensor is a useful tool for investigating ATP-relevant biological processes via the electrochemical method.

show abstract

ATP depletion plays a pivotal role in self‐incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes

et al. 2022

View full text Add to dashboard Cite

Self-incompatibility (SI) involves specific interactions during pollination to reject incompatible ('self') pollen, preventing inbreeding in angiosperms. A key event observed in pollen undergoing the Papaver rhoeas SI response is the formation of punctate F-actin foci.Pollen tube growth is heavily energy-dependent, yet ATP levels in pollen tubes have not been directly measured during SI. Here we used transgenic Arabidopsis lines expressing the Papaver pollen S-determinant to investigate a possible link between ATP levels, cytosolic pH ([pH] cyt ) and alterations to the actin cytoskeleton.We identify for the first time that SI triggers a rapid and significant ATP depletion in pollen tubes. Artificial depletion of ATP triggered cytosolic acidification and formation of actin aggregates. We also identify in vivo, evidence for a threshold [pH] cyt of 5.8 for actin foci formation. Imaging revealed that SI stimulates acidic cytosolic patches adjacent to the plasma membrane.In conclusion, this study provides evidence that ATP depletion plays a pivotal role in SI upstream of programmed cell death and reveals a link between the cellular energy status, cytosolic acidification and alterations to the actin cytoskeleton in regulating Papaver SI in pollen tubes.

show abstract

Cellular Energetics: Actin and Myosin Abstain from ATP during Starvation

Abstract: Energy is required for all cellular functions and diverse mechanisms allow the cell to overcome acute energy starvation. A new study reveals that starved cells inhibit type V myosins and actin depolymerization, measures that may conserve energy while temporarily retaining cell polarity.

Cited by 3 publications

References 20 publications

Effects of energy metabolism on the mechanical properties of breast cancer cells

Effects of energy metabolism on the mechanical properties of breast cancer cells

Electrochemical Nanoaptasensor for Continuous Monitoring of ATP Fluctuation at Subcellular Level

ATP depletion plays a pivotal role in self‐incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes

Contact Info

Product

Resources

About