“…Skeletal muscle contains not only myogenic cells but also nonmyogenic cells such as endothelial cells, pericytes, smooth muscle cells, fibroblasts, and glial cells. However, none of clusters 6, 10, 11, 13, 14, and 15 appeared to be these nonmyogenic cells based on the expression levels of marker genes such as CDH5 and PECAM1 for endothelial cells (Elmentaite et al, 2021), NOTCH3 and MCAM for pericytes (Elmentaite et al, 2021), ACTA2 and MYH11 for smooth muscle cells (Kumar et al, 2017), COL1A and S100A4 for fibroblasts (Kumar et al, 2017), and FOXD3 and SOX10 for glial cells (Elmentaite et al, 2021) in these clusters (Supplementary File S2). It is possible that clusters 6, 10, 11, 13, 14, and 15 represented novel cell types in bovine skeletal muscle co-isolated with satellite cells or that they were inaccurately clustered by the computational program used.…”