Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

Abstract

-The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

Plant regeneration, developmental pattern and genetic fidelity of somatic embryogenesis derived Musa spp.

“…The addition of glutamine to the M3 media may contribute to some amount of nitrogen source in the medium, which may have helped in improving the rate of somatic embryo maturation. The presence of glutamine facilitated completion of maturation of somatic embryos as reported by Morais-Lino et al [25]. The results indicated that exogenous supplementation of amino acid can serve as a nitrogen source for the synthesis of the protein which is important for embryo differentiation and maturation.…”

Histological and ultrastructural analysis of the Banana cv. Prata-Anã embryogenic calluses and cell suspension

“…Callus with four months, obtained on the first experiment, were transferred to Erlenmeyer flasks (125 mL) containing 15 mL of different culture mediums: MRT medium, (½ MS medium, 8.8 µM BA, 1.34 µM de NAA, 554.3 µM inositol, 400 mg L-1 malt, 100 mg L-1 de casein and 1 mL of vitamins solution (1g thiamin, 0.1 g pyridoxine, 0.1 g nicotinic acid and 0.1 mg L-1 glycine), MGM medium (1/2 MS medium, 2.57 µM AIA and 1.11 µM BA), CNPMF medium (MS medium, 4.52 µM 2,4-D, 100 mg L-1 glutamine, 10 mg L-1 ascorbic acid, 44.5 g L-1 sucrose and pH 5.3) (Morais-Lino et al, 2008) or MA2 medium (MS salt medium, vitamins (1.0 mg.L-1 biotin, 2.0 mg L-1 glycine, 0.5 mg L-1 nicotinic acid, 0.5 mg L-1 pyridoxine.HCl, 0.1 mg L-1 thiamin.HCl), 100 mg L-1 inositol, 100 mg L-1 malt extract, 100 mg L-1 glutamine, 45 g L-1 sucrose, 4.52 µM 2,4-D and pH 5.3 (Strosse et al, 2003). The pH was adjusted to 5.8 before autoclaving at 121 °C for 20 min.…”

Enhanced Secondary Somatic Embryogenesis in Suspension Culture of Four Diploid Banana Cultivars from Kerala

“…The cell suspensions with high regeneration capacity were commonly used for mass clonal propagation and these cells were also the only source to regenerate plant materials for inducing mutations and genetic engineering (Strosse et al, 2006). In situ gene expression analysis resolved the contradictory relationship between expression of developmental marker genes and cell fate in different culture systems (Miguel and Marum, 2011) and provided a more exact description of when and how embryogenic cells are formed in culture (Soriano et al, 2014). In the present investigation, secondary somatic embryos were observed during SEM studies of embryogenic callus.…”

“…Xu et al (2005) established embryogenic cell suspensions and plant regeneration of the dessert banana 'Williams' (Musa AAA, group). Morias -Lino et al (2008) reported cell suspension culture and plant regeneration of a Brazilian plantain, cultivar 'Terra' from immature male flowers, and secondary somatic embryos were reported from the suspension culture. Molecular genetics studies have also shown that ectopic expression of a specific embryoand meristem-expressed transcription factors or loss of certain chromatin-modifying proteins induces spontaneous somatic embryogenesis (Horstman et al, 2017).…”