Cell Surface Markers in Colorectal Cancer Prognosis

Belov, Larissa; Zhou, Jerry; Christopherson, Richard I.

doi:10.3390/ijms12010078

Cited by 57 publications

(45 citation statements)

References 265 publications

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“…These data suggest that EpCAM+CD44+ may be used as a reliable marker of colorectal CSCs [16]. More importantly, scientists have demonstrated that the combined and sequential use of CSC markers led to higher purified CSC subpopulations than any single biomarker [43, 44]. To date, no study has investigated the characteristics of colorectal CSCs expressing Lgr5 combined with CD44 and EpCAM.…”

Section: Discussionmentioning

confidence: 99%

Lgr5+CD44+EpCAM+ Strictly Defines Cancer Stem Cells in Human Colorectal Cancer

Leng

Xia

Chen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Although EpCAM+CD44+ cells exhibit more stem-like properties than did EpCAM-CD44- cells, the specificity of EpCAM combined with CD44 in defining CSCs needs further improvement. Lgr5 is used as a biomarker to isolate cancer stem cells (CSCs) in colorectal cancer. However, it remains unclear whether Lgr5, along with EpCAM and CD44, can further identify and define CSCs in colorectal cancer. Methods: Lgr5+CD44+EpCAM+, Lgr5+CD44+EpCAM-, Lgr5+CD44-EpCAM+, Lgr5-CD44+EpCAM+, and Lgr5-CD44-EpCAM-cells were separately isolated using fluorescence-activated cell sorting (FACS). Colony formation, self-renewal, differentiation, and tumorigenic properties of these cells were investigated through in vitro experiments and in vivo tumor xenograft models. The expression of stemness genes and CSC- and epithelial-mesenchymal transition (EMT)-related genes, such as KLF4, Oct4, Sox2, Nanog, CD133, CD44, CD166, ALDH1, Lgr5, E-cadherin, ZO-1, Vimentin, Snail, Slug, and Twist, was examined using real-time PCR. Results: Lgr5-positive subpopulations exhibited higher capacities for colony formation, self-renewal, differentiation, and tumorigenicity as well as higher expression of stemness genes and mesenchymal genes and lower expression of epithelial genes than did Lgr5-negative subpopulations. Conclusion: Our data revealed that tumorigenic cells were highly restricted to Lgr5-positive subpopulations. Most importantly, Lgr5+CD44+EpCAM+ cells exhibited more pronounced CSC-like traits than did any other subpopulation, indicating that Lgr5 combined with CD44 and EpCAM can further improve the stem-like traits of CSCs in colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Lgr5+CD44+EpCAM+ Strictly Defines Cancer Stem Cells in Human Colorectal Cancer

Leng

Xia

Chen

et al. 2018

Cell Physiol Biochem

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“…It is the most frequent cancer after lung, breast, and ovarian cancer [2,3]. The disease occurs more commonly in the elderly population [4,5].…”

Section: Discussionmentioning

confidence: 99%

Comparison of clinicopathologic features of young and older patients with sporadic colorectal adenocarcinoma

Gönüllü¹,

Gönüllü²,

Tiryaki³

et al. 2014

CMJ

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Aim. There is still considerable controversy and debate regarding the features of colorectal cancer in young and older patients. In the present study, the influence of age on clinicopathological characteristics of sporadic colorectal cancer is analyzed retrospectively. Method. A total of 170 cases of colectomy for adenocarcinoma in Kocaeli University Medical School, Turkey, Department of Surgery between January 2001 and September 2005 were studied. Results. Of the 170 patients, 40 (23.5%) were in the ≤50 age group and 130 (76.5%) in the >50 age group were included in the study. Statistical tests were applied for the variables. No differences were found between the young and older patients in respect of clinical features, histopathological features, surgical procedures, or post-operative complications. Conclusion. According to this study, there are no differences between young and older patients with sporadic colorectal adenocarcinoma in respect of clinical features, histopathological features, surgical procedures, or post-operative complications. The differences found previously as recorded in the literature may be associated with genetic variations.

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“…Biological markers, such as the DNA mismatch repair genes DCC, NM23-H1 and K-Ras have been verified as being able to predict disease relapse (7)(8)(9)(10). The application of those molecular markers at the clinical level remains limited, however, due to limitations in their reproducibility and independency from other major prognosticators.…”

Section: Introductionmentioning

confidence: 99%

Association of Wilms' tumor 1 gene single-nucleotide polymorphism rs16754 with colorectal cancer

Sangkhathat

Maneechay

Chaiyapan

et al. 2015

Molecular and Clinical Oncology

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Abstract. Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality worldwide. Our recent study demonstrated that the expression of Wilms' tumor 1 gene (WT1) is associated with surgical outcome in CRC patients. The present study aimed to investigate the genetic association of the single-nucleotide polymorphism rs16754 in the WT1 gene with the occurrence of CRC, using an age-matched case-control study design. In addition, the correlation between genotype and WT1 expression was investigated. Genomic DNA samples from 104 CRC cases, aged 15-65 years, and 208 healthy controls, were genotyped for rs16754 using the TaqMan genotyping method. The genotype distribution conformed to the Hardy-Weinberg equilibrium (P=0.80). The overall minor allele frequency (MAF) of rs16754 (allele A) was 0.33. The MAF among CRC cases was significantly higher compared with that in controls (0.39 vs. 0.31, respectively; P=0.03). The AA genotype was significantly associated with the disease (odds ratio = 2.51, 95% confidence interval: 1.24-5.07, P=0.01). Cases with the AA genotype exhibited a significantly poorer 3-year overall survival (60%), compared with those with the GG or GA genotypes (80%) (log-rank test, P<0.01). Reverse transcription quantitative polymerase chain reaction analysis demonstrated that the expression of WT1 in tumor tissues was higher compared with that in normal tissue; however, there were no significant differences in its expression among different genotypes. Therefore, rs16754 was found to be associated with the occurrence and prognosis of CRC in our subjects.

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Cell Surface Markers in Colorectal Cancer Prognosis

Cited by 57 publications

References 265 publications

Lgr5+CD44+EpCAM+ Strictly Defines Cancer Stem Cells in Human Colorectal Cancer

Lgr5+CD44+EpCAM+ Strictly Defines Cancer Stem Cells in Human Colorectal Cancer

Comparison of clinicopathologic features of young and older patients with sporadic colorectal adenocarcinoma

Association of Wilms' tumor 1 gene single-nucleotide polymorphism rs16754 with colorectal cancer

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