Cell orientation and cytoskeleton organisation on ground titanium surfaces

Eisenbarth, E.; Linez, P.; Biehl, V.; Velten, Dirk; Brème, J.; Hildebrand, Hartmut F.

doi:10.1016/s1389-0344(02)00028-x

Cited by 94 publications

(62 citation statements)

References 6 publications

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“…The percentage of aligned cells did not signifi cantly change with the distance of the Ti oxide lines within the range of 2-20 m. This was similar to data of Teixeira et al [2003], who found the percentage of aligned corneal epithelial cells to be constant on silicone patterns with pitches (sum of ridge + groove widths) ranging from 0.4 to 2 m. The portion of cells aligning to a structured surface depends on the peakto-valley height, with more orientated cells on the higher structures or the deeper grooves. The oxide lines used in our experiments were only 12 nm in height, which is very low compared to the micrometer-sized groove-and-ridge pattern used in other studies [Eisenbarth et al, 2002: 0.08-1.4 m; Soboyejo et al, 2002: 8-12 m;Lu and Leng, 2003: 2-10 m]. Because in the majority of the cells, alignment was parallel to the lines, cells may recognize the nanosize difference in height between Ti bulk surface and Ti oxide lines.…”

Section: Surface Structure and Cell Orientation: Mechanical Guidance?mentioning

confidence: 73%

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Monsees¹,

Barth²,

Tippelt³

et al. 2005

Cells Tissues Organs

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To test nanosize surface patterning for application as implant material, a suitable titanium composition has to be found first. Therefore we investigated the effect of surface chemistry on attachment and differentiation of osteoblast-like cells on pure titanium prepared by pulsed laser deposition (TiPLD) and different Ti alloys (Ti6Al4V, TiNb30 and TiNb13Zr13). Early attachment (30 min) and alkaline phosphatase (ALP) activity (day 5) was found to be fastest and highest, respectively, in cells grown on TiPLD and Ti6Al4V. Osteoblasts seeded on TiPLD produced most osteopontin (day 10), whereas expression of this extracellular matrix protein was an order of magnitude lower on the TiNb30 surface. In contrast, expression of the corresponding receptor, CD44, was not influenced by surface chemistry. Thus, TiPLD was used for further experiments to explore the influence of surface nanostructures on osteoblast adhesion, differentiation and orientation. By laser-induced oxidation, we produced patterns of parallel Ti oxide lines with different widths (0.2–10 µm) and distances (2–20 and 1,000 µm), but a common height of only 12 nm. These structures did not influence ALP activity (days 5–9), but had a positive effect on cell alignment. Two days after plating, the majority of the focal contacts were placed on the oxide lines. The portion of larger focal adhesions bridging two lines was inversely related to the line distance (2–20 µm). In contrast, the portion of aligned cells did not depend on the line distance. On average, 43% of the cells orientated parallel towards the lines, whereas 34% orientated vertically. In the control pattern (1,000 µm line distance), cell distribution was completely at random. Because a significant surplus of the cells preferred a parallel alignment, the nanosize difference in height between Ti surface and oxide lines may be sufficient to orientate the cells by contact guiding. However, gradients in electrostatic potential and surface charge density at the Ti/Ti oxide interface may additionally influence focal contact formation and cell guidance.

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Section: Surface Structure and Cell Orientation: Mechanical Guidance?mentioning

confidence: 73%

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Monsees¹,

Barth²,

Tippelt³

et al. 2005

Cells Tissues Organs

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“…Human gingival fibroblasts were seeded onto several kinds of ground titanium materials such as Ti, TiAl6V4 and TiTa30, and then the numbers of orientated cells were determined (Eisenbarth et al, 1996(Eisenbarth et al, , 2002. MC3T3 osteoblasts were seeded onto ground Ti6A14V substrates, and it was found that the adhesion, growth, and alignment of the cells were influenced by the surface topology (Linez-Bataillon et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Alignment of skeletal muscle myoblasts and myotubes using linear micropatterned surfaces ground with abrasives

Shimizu

Fujita

Nagamori

2009

Biotech & Bioengineering

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Alignment of cells plays a significant key role in skeletal muscle tissue engineering because skeletal muscle tissue in vivo has a highly organized structure consisting of long parallel multinucleated myotubes formed through differentiation and fusion of myoblasts. In the present study, we developed an easy, simple, and low-cost method for aligning skeletal muscle cells by using surfaces with linear microscale features fabricated by grinding. Iron blocks were ground in one direction with three kinds of abrasives (9 microm diamond suspension, #400 sandpaper, and #150 sandpaper) and then used as molds to make micropatterned polydimethylsiloxane (PDMS) substrates (type I, type II, and type III). Observation of the surface topography revealed that the PDMS substrates exhibited different degree of mean roughness (Ra), 0.03 microm for type I, 0.16 microm for type II, and 0.56 microm for type III, respectively. Murine skeletal muscle cell line C2C12 myoblasts were cultured and differentiated on the patterned PDMS substrates, and it was examined whether the alignment of C2C12 myoblasts and myotubes was possible. Although the cell growth and differentiation on the three types of patterned substrates were similar to those on the flat PDMS substrate as a control, the alignment of both C2C12 myoblasts and myotubes was obviously observed on types II and III, but not on type I or the control substrate. These results indicate that surfaces ground with abrasives will be useful for fabricating aligned skeletal muscle tissues.

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“…A key factor that influences the adherence of Vero cells is the substratum surface charges 1,15,35,36 . Dxygen and nitrogen plasma treatment improved the time span (2 hours) for interaction between Vero cells and the PLGA membrane by promoting anchorage to the substrate, spreading and proliferation.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

et al. 2013

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Cell adhesion on materials surface is critical because this phenomenon occurs before other events, as cell spreading, cell migration and cell differentiation. It is commonly accepted that the adhesion of cells on solid substrate is influenced by several substratum surface properties, such as wettability, surface charge, roughness and topography. Plasma technique is a convenient method for modifying surface properties of materials without affecting physical properties. In this study, poly(lactide-co-glycolide), PLGA, membranes were modified by oxygen and nitrogen plasma to improve polymer hydrophilicity and verify their effect on Vero cells culture. The PLGA membranes, which were characterized by SEM and contact angle, showed increased surface rugosity and narrower contact angles. Cell adhesion, cytotoxicity assay, SEM and cytochemistry analysis showed that plasma treatment was beneficial to cell growth by improving cell-polymer interaction.

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Cell orientation and cytoskeleton organisation on ground titanium surfaces

Cited by 94 publications

References 6 publications

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Alignment of skeletal muscle myoblasts and myotubes using linear micropatterned surfaces ground with abrasives

Benefits of oxygen and nitrogen plasma treatment in Vero cell affinity to poly(lactide-co-glycolide acid)

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