Cell–material interactions

Donnelly, Hannah; Vermeulen, Steven; Tsimbouri, Monica P.; Dalby, Matthew J.

doi:10.1016/b978-0-12-824459-3.00008-1

Cited by 3 publications

(2 citation statements)

References 39 publications

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“…[ 11 ] This progress should parallel the fundamental understanding of cell–material interactions, critical to the success of any tissue‐engineered construct. [ 12 ]…”

Section: Biohybrid Implants As Lifelike Materialsmentioning

confidence: 99%

“…[11] This progress should parallel the fundamental understanding of cell-material interactions, critical to the success of any tissue-engineered construct. [12] One of the key developments in bottom-up TE is the ability to exploit the intrinsic self-assembling and self-organizing nature of biological materials and combine this with nanofabrication and microfabrication methods to develop functional biohybrid constructs. [8] Self-assembly is the prime example of bottom-up organization found in nature and we can now design novel self-assembling materials, often based on protein subunits that spontaneously assemble and disassemble in different environments.…”

Section: Technological Approaches For the Development Of Biohybrid Im...mentioning

confidence: 99%

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Lifelike Transformative Materials for Biohybrid Implants: Inspired by Nature, Driven by Technology

Fernández‐Colino

Kießling

Slabu

et al. 2023

Adv Healthcare Materials

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Today's living world is enriched with a myriad of natural biological designs, shaped by billions of years of evolution. Unraveling the construction rules of living organisms offers the potential to create new materials and systems for biomedicine. From the close examination of living organisms, several concepts emerge: hierarchy, pattern repetition, adaptation, and irreducible complexity. All these aspects must be tackled to develop transformative materials with lifelike behavior. This perspective article highlights recent progress in the development of transformative biohybrid systems for applications in the fields of tissue regeneration and biomedicine. Advances in computational simulations and data‐driven predictions are also discussed. These tools enable the virtual high‐throughput screening of implant design and performance before committing to fabrication, thus reducing the development time and cost of biomimetic and biohybrid constructs. The ongoing progress of imaging methods also constitutes an essential part of this matter in order to validate the computation models and enable longitudinal monitoring. Finally, the current challenges of lifelike biohybrid materials, including reproducibility, ethical considerations, and translation, are discussed. Advances in the development of lifelike materials will open new biomedical horizons, where perhaps what is currently envisioned as science fiction will become a science‐driven reality in the future.

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“…[ 11 ] This progress should parallel the fundamental understanding of cell–material interactions, critical to the success of any tissue‐engineered construct. [ 12 ]…”

Section: Biohybrid Implants As Lifelike Materialsmentioning

confidence: 99%

Section: Technological Approaches For the Development Of Biohybrid Im...mentioning

confidence: 99%

Lifelike Transformative Materials for Biohybrid Implants: Inspired by Nature, Driven by Technology

Fernández‐Colino

Kießling

Slabu

et al. 2023

Adv Healthcare Materials

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Computer Vision for Substrate Detection in High‐Throughput Biomaterial Screens Using Bright‐Field Microscopy

Owen,

Nasir,

Amer

et al. 2024

Advanced Intelligent Systems

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High‐throughput screening (HTS) can be used when ab initio information is unavailable for rational design of new materials, generating data on properties such as chemistry and topography that control cell behavior. Biomaterial screens are typically fabricated as microarrays or “chips,” seeded with the cell type of interest, then phenotyped using immunocytochemistry and high‐content imaging, generating vast quantities of image data. Typically, analysis is only performed on fluorescent cell images as it is relatively simple to automate through intensity thresholding of cellular features. Automated analysis of bright‐field images is rarely performed as it presents an automation challenge as segmentation thresholds that work in all images cannot be defined. This limits the biological insight as cell response cannot be correlated to specifics of the biomaterial feature (e.g., shape, size) as these features are not visible on fluorescence images. Computer Vision aims to digitize tasks humans do by sight, such as identify objects by their shape. Herein, two case studies demonstrate how open‐source approaches, (region‐based convolutional neural network and algorithmic [OpenCV]), can be integrated into cell‐biomaterial HTS analysis to automate bright‐field segmentation across thousands of images, allowing rapid, spatial definition of biomaterial features during cell analysis for the first time.

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3D-Printed Silk Proteins for Bone Tissue Regeneration and Associated Immunomodulation

Waidi,

Debnath,

Datta

et al. 2024

Biomacromolecules

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Current bone repair methods have limitations, prompting the exploration of innovative approaches. Tissue engineering emerges as a promising solution, leveraging biomaterials to craft scaffolds replicating the natural bone environment, facilitating cell growth and differentiation. Among fabrication techniques, three-dimensional (3D) printing stands out for its ability to tailor intricate scaffolds. Silk proteins (SPs), known for their mechanical strength and biocompatibility, are an excellent choice for engineering 3D-printed bone tissue engineering (BTE) scaffolds. This article comprehensively reviews bone biology, 3D printing, and the unique attributes of SPs, specifically detailing criteria for scaffold fabrication such as composition, structure, mechanics, and cellular responses. It examines the structural, mechanical, and biological attributes of SPs, emphasizing their suitability for BTE. Recent studies on diverse 3D printing approaches using SPs-based for BTE are highlighted, alongside advancements in their 3D and four-dimensional (4D) printing and their role in osteo-immunomodulation. Future directions in the use of SPs for 3D printing in BTE are outlined.

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Cell–material interactions

Cited by 3 publications

References 39 publications

Lifelike Transformative Materials for Biohybrid Implants: Inspired by Nature, Driven by Technology

Lifelike Transformative Materials for Biohybrid Implants: Inspired by Nature, Driven by Technology

Computer Vision for Substrate Detection in High‐Throughput Biomaterial Screens Using Bright‐Field Microscopy

3D-Printed Silk Proteins for Bone Tissue Regeneration and Associated Immunomodulation

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