Cell loss and recovery in umbilical cord blood processing: a comparison of postthaw and postwash samples

Laroche, Vinçent; McKenna, David H.; Moroff, Gary; Schierman, Therese; Kadidlo, Diane; McCullough, Jeffrey

doi:10.1111/j.1537-2995.2005.00638.x

Cited by 96 publications

(101 citation statements)

References 30 publications

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“…These observations suggest the CB unit selection criteria should include CD34 þ cell counts despite the variability induced by multiple techniques used for cell numeration in CB banks. In contrast to previously published studies, 26,27 we observed a significant correlation prefreeze/post-thaw by the use of a single cytometry platform for the quality control step. As suggested in preliminary studies about CD34 þ cell measurement 19,20 and CB thawing, 28 our results confirm, under transplantation conditions, that standardization of CD34 þ cell numeration techniques and use of single platform cytometry are necessary to better estimate the CD34 þ cell influence in CB transplantation.…”

Section: Discussioncontrasting

confidence: 99%

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Lemarié

Esterni

Calmels

et al. 2007

Bone Marrow Transplant

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Cord blood (CB) units are increasingly used for allogeneic transplantation. Cell dose, a major factor for CB selection, is evaluated before freezing by each CB bank, using various techniques. This may introduce variability and affect the prediction of cell recovery after thawing, or haematopoietic reconstitution. Forty-two children were transplanted at the same institution with unrelated CB units. All units were thawed and evaluated at the same cell therapy facility, using standard procedures. We investigated: (i) factors that affect cell loss after thawing, and (ii) the importance of CD34 þ cell doses. Prefreeze and postthaw CD34 þ cell doses were statistically correlated, thus suggesting that variability in numeration techniques used by different CB banks does not compromise the biological and clinical value of these figures. CD34 þ cell recovery appeared to be correlated with the absolute number of CD34 þ cells per frozen bag. Infused CD34 þ is the cell dose that better correlates with platelet reconstitution delay; in addition, when using a quartile comparison, haematopoietic recovery appeared to be related with prefreeze and post-thaw CD34 þ cell doses. We conclude that enumeration of CD34 þ cells in CB units is of biological significance, and may help select CB units and identify patients at risk of delayed recovery.

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Section: Discussioncontrasting

confidence: 99%

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Lemarié

Esterni

Calmels

et al. 2007

Bone Marrow Transplant

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“…In most studies, CD34 + cell selection is done before initiating cell culture [46], but the CD34 + cell selection itself is associated with a substantial loss of progenitor cells. This cell loss, which may not be significant for smaller children, may become critical in reaching a suitable dose for transplant in older children and adults [47].…”

Section: Ex Vivo Expansion Of Hscmentioning

confidence: 99%

“…Laroche et al [47] stated that thawing and washing result in loss of cells approaching 20% when compared with pre-freeze counts, with the wash step responsible for nearly half of this cell loss. However, Beshlawy et al [48], using umbilical cord blood derived hematopoietic stem cells, detected mean fold expansion of 6.64 ± 3.34 with stem cell factor alone, 7.38 ± 2.86 with both stem cell factor and IL-3, and 8.11 ± 4.49 with IL-3 alone after 2 days culture of the samples frozen for 2 weeks.…”

Section: Ex Vivo Expansion Of Hscmentioning

confidence: 99%

Hematopoietic stem cells from peripheral blood the perspective of non-mobilized peripheral blood

Katsares¹,

Paparidis²,

Nikolaidou³

et al. 2010

Health

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The peripheral blood is a major source of hematopoietic stem cells. Almost for two decades the peripheral blood has been mobilized, in order to enhance the CD34 + concentration. The isolated stem cells from the mobilized peripheral blood are used as an alternative, or in addition to bone marrow derived stem cells. In this paper, a new perspective is being discussed; the use of non-mobilized peripheral blood as an alternative source for hematopoietic progenitor cells. The number of isolated hematopoietic stem cells is evaluated using flow cytometry. The viability can be evaluated using the trypan blue exclusion test, the flow cytometry or automated assays. The isolated hematopoietic stem cells could be used for ex vivo expansion either in static systems or in proper bioreactor systems, prior to cryopreservation and/or transplantation.

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“…Limitations to UCB transplantation (UCBT) for adult patients have been identified, including delayed engraftment due to the low dose of infused stem cells. 1 Unnecessary cell loss during processing, estimated to be around 20% using washing techniques, 2 can further reduce the infused stem cell dose. Larger umbilical cord grafts have been shown to improve time to engraftment and decrease transplant-related mortality.…”

Section: Introductionmentioning

confidence: 99%

A prospective investigation of cell dose in single-unit umbilical cord blood transplantation for adults with high-risk hematologic malignancies

Sobol

Kliethermes

et al. 2015

Bone Marrow Transplant

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Umbilical cord blood (UCB) as an allogeneic transplant source is generally limited to units with pre-cryopreservation total nucleated cell (TNC) doses ⩾ 2.5 × 10 7 NC/kg. We prospectively investigated single UCB transplantation, with cord units as low as 1 × 10 7 NC/kg, all processed with post-thaw albumin-dextran dilution. We transplanted 104 adult patients with 84% having relapsed/ refractory disease. The median TNC dose was 2.1 × 10 7 NC/kg (range: 1.0-4.4 × 10 7 ) and median CD34+ cell dose was 1.0 × 10 5 /kg (range: 0.0-3.7 × 10 5 /kg). Post-manipulation cell recovery and viability were 96% and 99%, respectively. Median times to neutrophil and platelet engraftment were 16 and 43 days, respectively. Univariate factors predicting neutrophil engraftment included TNC (P = 0.03) and CD34+ cell dose (P = 0.01). CD34+ dose predicted platelet engraftment (P o0.001). In multivariate analysis, CD34+ dose remained significant for neutrophil and platelet engraftment (P o0.0001 and P o 0.0001, respectively). The 100-day and 1-year overall survival were 70% and 46%, respectively (95% confidence interval: 36%-56% at 1 year). The subset transplanted with 1-1.5 × 10 7 NC/kg had similar 100-day and 1-year survivals of 73% and 45%, respectively. Single-unit UCB transplantation using small units, processed as described, leads to favorable engraftment and acceptable outcomes in poor prognosis patients. CD34+ cell dose (⩾1.5 × 10 5 /kg) helps predict faster engraftment and can aid in graft selection.

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Cell loss and recovery in umbilical cord blood processing: a comparison of postthaw and postwash samples

Cited by 96 publications

References 30 publications

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Hematopoietic stem cells from peripheral blood the perspective of non-mobilized peripheral blood

A prospective investigation of cell dose in single-unit umbilical cord blood transplantation for adults with high-risk hematologic malignancies

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