Cell-free protein synthesis in hydrogel materials

Whitfield, Colette J.; Banks, Alice M.; Durá, Gema; Love, John; Fieldsend, Jonathan E.; Goodchild, Sarah A.; Fulton, David A.; Howard, Thomas P.

doi:10.1039/d0cc02582h

Cited by 26 publications

(38 citation statements)

References 20 publications

(17 reference statements)

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“…PEG is known to be a critical component in cell-free systems, playing a role in macromolecular crowding to enhance intermolecular associations. Our results agree with previous findings that, although there is a benefit to including PEG, high concentrations can negatively impact reaction performance [41] , [42] , [43] . This was most apparent when considering reaction rate – curvature was detected in the DSD, and the RSM also identified a significant inverse interaction between PEG-8000 and CFE suggesting protein concentration in the CFE may have comparable crowding effects to the those contributed by PEG.…”

Section: Discussionsupporting

confidence: 93%

Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions

Banks

Whitfield

Brown

et al. 2022

Computational and Structural Biotechnology Journal

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Section: Discussionsupporting

confidence: 93%

Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions

Banks

Whitfield

Brown

et al. 2022

Computational and Structural Biotechnology Journal

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“…The next challenge is to move from diagnostics to surveillance. The potential to take such devices and embed them in materials, such as paper or hydrogels [99], allows such diagnostics to become surveillance systems with a physical presence in the field rather than a complex specialist diagnostic ( Figure 5C). The goal is to develop biosensors that can be embedded in the agricultural environment that monitor crop pathogens, or their vectors, and that are robust enough to handle complex samples (A) Synthetic gene circuits can be designed to produce different outputs in response to a range of biotic and abiotic targets.…”

Section: Potential Of Synthetic Biology For Self-reportingmentioning

confidence: 99%

Plant pest surveillance: from satellites to molecules

Silva

Tomlinson

Onkokesung

et al. 2021

Emerging Topics in Life Sciences

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Plant pests and diseases impact both food security and natural ecosystems, and the impact has been accelerated in recent years due to several confounding factors. The globalisation of trade has moved pests out of natural ranges, creating damaging epidemics in new regions. Climate change has extended the range of pests and the pathogens they vector. Resistance to agrochemicals has made pathogens, pests, and weeds more difficult to control. Early detection is critical to achieve effective control, both from a biosecurity as well as an endemic pest perspective. Molecular diagnostics has revolutionised our ability to identify pests and diseases over the past two decades, but more recent technological innovations are enabling us to achieve better pest surveillance. In this review, we will explore the different technologies that are enabling this advancing capability and discuss the drivers that will shape its future deployment.

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“…Various cell‐free biosensors have been demonstrated to detect antibiotics (Jung et al., 2020 ), pathogens (Pardee et al., 2016 ; Takahashi et al., 2018 ), toxic substances (Lopreside et al., 2019 ; Jung et al., 2020 ), etc. Moreover, cell‐free extracts comprising genetic sensors could be embedded on paper, providing a portable platform for easy‐to‐use and cost‐effective on‐site screening (Pardee et al., 2016 ; Takahashi et al., 2018 ), or in hydrogels acting as environment‐responsive biomaterials (Whitfield et al., 2020 ).…”

Section: Synthetic Biology Provides Novel Strategies To Overcome Field‐deployable Limitations Of Living Sensorsmentioning

confidence: 99%

Programming living sensors for environment, health and biomanufacturing

Wan

Saltepe

2021

Microbial Biotechnology

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Synthetic biology offers new tools and capabilities of engineering cells with desired functions for example as new biosensing platforms leveraging engineered microbes. In the last two decades, bacterial cells have been programmed to sense and respond to various input cues for versatile purposes including environmental monitoring, disease diagnosis and adaptive biomanufacturing. Despite demonstrated proof-of-concept success in the laboratory, the realworld applications of microbial sensors have been restricted due to certain technical and societal limitations. Yet, most limitations can be addressed by new technological developments in synthetic biology such as circuit design, biocontainment and machine learning. Here, we summarize the latest advances in synthetic biology and discuss how they could accelerate the development, enhance the performance and address the present limitations of microbial sensors to facilitate their use in the field. We view that programmable living sensors are promising sensing platforms to achieve sustainable, affordable and easy-to-use on-site detection in diverse settings.

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Cell-free protein synthesis in hydrogel materials

Abstract: Fabrication of macro-scale polysaccharide, proteinaceous, micellular and covalently crosslinked hydrogels for housing cell-free protein synthesis reactions.

Cited by 26 publications

References 20 publications

Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions

Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions

Plant pest surveillance: from satellites to molecules

Programming living sensors for environment, health and biomanufacturing

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