2012
DOI: 10.1016/j.cell.2012.04.016
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Abstract: Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mR… Show more

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Cited by 694 publications
(729 citation statements)
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“…In the case of FUS, multisite phosphorylation of the LC dramatically increases the effective number of negatively charged residues in the domain. Complementing previous findings that DNA‐PK treatment disrupts FUS LC recruitment to hydrogel models of granules (Han et al , 2012), here we demonstrate that phosphorylation and phosphomimetic substitution of FUS LC disrupts domain self‐interaction and LLPS. The LC is required for nuclear self‐association of FUS into chromatin‐binding dynamic puncta (Yang et al , 2014) and cytoplasmic granules (Shelkovnikova et al , 2014), as well as robust recruitment at sites of DNA damage (Mastrocola et al , 2013; Altmeyer et al , 2015).…”
Section: Discussionsupporting
confidence: 88%
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“…In the case of FUS, multisite phosphorylation of the LC dramatically increases the effective number of negatively charged residues in the domain. Complementing previous findings that DNA‐PK treatment disrupts FUS LC recruitment to hydrogel models of granules (Han et al , 2012), here we demonstrate that phosphorylation and phosphomimetic substitution of FUS LC disrupts domain self‐interaction and LLPS. The LC is required for nuclear self‐association of FUS into chromatin‐binding dynamic puncta (Yang et al , 2014) and cytoplasmic granules (Shelkovnikova et al , 2014), as well as robust recruitment at sites of DNA damage (Mastrocola et al , 2013; Altmeyer et al , 2015).…”
Section: Discussionsupporting
confidence: 88%
“…However, DNA‐PK treatment in vitro followed by either tandem mass spectroscopy or Edman sequencing resulted in identification of only five phosphorylation sites in FUS: S26, S42, S61, S84, and S131 (Gardiner et al , 2008; Han et al , 2012). None of the four conserved TQ sites were detected.…”
Section: Resultsmentioning
confidence: 99%
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