Cell detection by surface imprinted polymers (SIPs) — A study of the sensor surface by optical and dielectric relaxation spectroscopy

Gennaro, Alessia; Yongabi, Derick; Deschaume, Olivier; Bartic, Carmen; Wagner, Patrick; Wübbenhorst, Michael

doi:10.1109/tdei.2018.007092

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…Surface-imprinted polyurethane layers were synthesized as described in previous work. [61][62][63][64] Hydrophilic glass substrates were prepared by exposing microscope cover-glass chips (Glaswarenfabrik Karl Hecht GmbH & Co, Sondheim, Germany) to UV ozone (UVO-Cleaner, Jelight Company Inc., CA, USA) for 90 min and cleaned in absolute ethanol and acetone. Hydrophobic glass substrates were prepared by silanization and involved immersing microscope cover glass chips into a 0.5% (v/v) solution of noctadecyltrichlorosilane (OTS) in toluene for 1 h and subsequently rinsing three times with water-free toluene.…”

Section: Methodsmentioning

confidence: 99%

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Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

et al. 2022

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Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label-free methods without (bio)-chemical markers or surface-engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label-and receptor-free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell-detachment with sharply defined time-patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell-type-specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic-type oscillations are observed during detachment of mammalian and yeast-cell ensembles, providing additional cell-specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs.

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Section: Methodsmentioning

confidence: 99%

“…To compare cell adhesion and detachment between plain polyurethane layers and polyurethane layers with altered chemical and topographical features, surface imprinting was employed for the latter. Surface‐imprinted polyurethane layers were synthesized as described in previous work [61 – 64 . ]…”

Section: Methodsmentioning

confidence: 99%

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

et al. 2022

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“…Here, a peak in heating is found slightly above 80 °C, which is consistent with the results of a previous study on this specific polyurethane type that reported also a glass transition temperature of 80 °C. 41) Strikingly, the peak temperature in the cooling run appears about 20 °C higher as compared to the heating run, which implies that the sample underwent severe post-curing processes at elevated temperatures.…”

Section: Cure Kinetics and Glass Transition Effects Of Isothermally C...mentioning

confidence: 97%

An imaging study and spectroscopic curing analysis on polymers for synthetic whole-cell receptors for bacterial detection

Givanoudi

Gennaro

Yongabi

et al. 2020

Jpn. J. Appl. Phys.

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Surface imprinted polymers (SIPs) are versatile receptors in bioanalytical applications for the selective detection of cells and microorganisms such as bacteria. One of the synthesis routes is the so-called stamping method in which template bacteria are pressed mechanically into a thin, gel-like polyurethane layer, which is then cured in the presence of the templates to create cell-specific binding pockets on the polymer. The present work focusses on two specific steps of the imprinting protocol: first, we evaluate the sedimentation of two different groups of bacteria, Escherichia coli and Escherichia blattae, on silicone stamps with respect to the resulting surface coverage, which is a key factor for the efficiency of the imprinting process. Second, we analyse the temperature dependence of the thermal- and dielectric properties of polyurethane during curing by dielectric- and pyroelectric spectroscopy. This provides information for improved curing protocols and on the stability of SIP materials at elevated temperatures.

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“…For instance, techniques that employ changes in the electrical impedance properties at the chip-liquid-cell interfaces. [49][50][51][52] This may help to further elucidate the differences in adhesion mechanisms between species and strains, because adhesion is a multifactorial process involving cell surface characteristics such as hydrophobicity, different types and number of appendages, such as fimbriae, flagella, and/or curli, as well as EPS, as described for members of the Enterobacteriaceae. [53]…”

Section: Viscoelastic and Adhesion Monitoring Enable Label-free Bacte...mentioning

confidence: 99%

QCM‐D Viscoelastic and Adhesion Monitoring Facilitate Label‐Free and Strain‐Selective Bacterial Discrimination

Yongabi,

Khorshid,

Korbas

et al. 2023

Physica Status Solidi (a)

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Discriminating bacterial adhesion profiles at strain‐specific level is crucial for simulating and predicting infections and persistence, as well as developing more efficient antibacterial therapies. Herein, it is proposed that label‐ and receptor‐free bacterial discrimination can be achieved by dynamic viscoelastic and adhesion monitoring over specified timescales using the quartz crystal microbalance with dissipation monitoring (QCM‐D). Using two closely related E. coli strains, ATCC 8739 and JM109(DE3), it is shown that their viscoelastic and adhesion properties evolve in time through strain‐specific profiles that are clearly distinguishable over a period of 3–4 h. In addition, the viscoelasticity of both E. coli strains shows a strong strain‐specific dependence on the medium ionic strength, allowing to further amplify the differences in the bacterial adhesion signatures. Furthermore, the viscoelastic and adhesion behaviors of the two E. coli strains with two additional bacteria, Citrobacter freundii and Serratia marcescens, are compared. For all four bacteria, distinct viscoelastic profiles and adhesion fingerprints emerge over similar timescales that allow to reliably discriminate the various bacteria. These results and similar studies on other bacteria might have pharmacological benefits, for instance, by highlighting the role of bacterial–substrate adhesion and viscoelastic properties on disease pathogenesis and persistence, toward developing more effective therapies.

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Cell detection by surface imprinted polymers (SIPs) — A study of the sensor surface by optical and dielectric relaxation spectroscopy

Cited by 9 publications

References 20 publications

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification

An imaging study and spectroscopic curing analysis on polymers for synthetic whole-cell receptors for bacterial detection

QCM‐D Viscoelastic and Adhesion Monitoring Facilitate Label‐Free and Strain‐Selective Bacterial Discrimination

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