Cell detachment and cloning efficiency as parameters for cytotoxicity

In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test

“…Since a need for repeated establishment of fresh corneal cultures would be cumbersome, and would lead to great heterogeneity of starting material when prepared in different laboratories, the suitability of established cell lines for these studies is reassuring. Similar conclusions were arrived at by Litterst and Lichtenstein (1971) in their in vitro toxicity studies comparing HeLa cells and shortterm cultures of normal human fibroblasts, and by Reinhardt et al (1982), who used a baby hamster kidney cell line for quantification of cytotoxicity in vitro.…”

In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test

“…Since a need for repeated establishment of fresh corneal cultures would be cumbersome, and would lead to great heterogeneity of starting material when prepared in different laboratories, the suitability of established cell lines for these studies is reassuring. Similar conclusions were arrived at by Litterst and Lichtenstein (1971) in their in vitro toxicity studies comparing HeLa cells and shortterm cultures of normal human fibroblasts, and by Reinhardt et al (1982), who used a baby hamster kidney cell line for quantification of cytotoxicity in vitro.…”

Biological activity of organotin compounds—An overview

“…The incorporation of DNA, RNA, and protein precursors and also the production of cyclic AMP were markedly diminished in thymocytes by those trialkyltin homologs that also interfered with energy metabolism (Snoeij et al, 1986c, d). Antiproliferative effects of trialkyltin compounds were also described for a baby hamster kidney cell line (Reinhardt et al, 1982), for rabbit chondrocytes (Webber et al, 1985), and for rat skin, either in organ culture or in vivo (Kao et al, 1983;Middleton and Pratt, 1978). This property of the triorganotin compounds may very well be the cause of the drastic effects of TBTO on embryonic development of explants of mice in vitro, as was studied recently using limb bud organ cultures (Krowke et al, 1986).…”

In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test

“…Since a need for repeated establishment of fresh corneal cultures would be cumbersome, and would lead to great heterogeneity of starting material when prepared in different laboratories, the suitability of established cell lines for these studies is reassuring. Similar conclusions were arrived at by Litterst and Lichtenstein (1971) in their in vitro toxicity studies comparing HeLa cells and shortterm cultures of normal human fibroblasts, and by Reinhardt et al (1982), who used a baby hamster kidney cell line for quantification of cytotoxicity in vitro.…”

Biological activity of organotin compounds—An overview

“…The incorporation of DNA, RNA, and protein precursors and also the production of cyclic AMP were markedly diminished in thymocytes by those trialkyltin homologs that also interfered with energy metabolism (Snoeij et al, 1986c, d). Antiproliferative effects of trialkyltin compounds were also described for a baby hamster kidney cell line (Reinhardt et al, 1982), for rabbit chondrocytes (Webber et al, 1985), and for rat skin, either in organ culture or in vivo (Kao et al, 1983;Middleton and Pratt, 1978). This property of the triorganotin compounds may very well be the cause of the drastic effects of TBTO on embryonic development of explants of mice in vitro, as was studied recently using limb bud organ cultures (Krowke et al, 1986).…”

In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test

“…Since a need for repeated establishment of fresh corneal cultures would be cumbersome, and would lead to great heterogeneity of starting material when prepared in different laboratories, the suitability of established cell lines for these studies is reassuring. Similar conclusions were arrived at by Litterst and Lichtenstein (1971) in their in vitro toxicity studies comparing HeLa cells and shortterm cultures of normal human fibroblasts, and by Reinhardt et al (1982), who used a baby hamster kidney cell line for quantification of cytotoxicity in vitro.…”

Biological activity of organotin compounds—An overview

“…The incorporation of DNA, RNA, and protein precursors and also the production of cyclic AMP were markedly diminished in thymocytes by those trialkyltin homologs that also interfered with energy metabolism (Snoeij et al, 1986c, d). Antiproliferative effects of trialkyltin compounds were also described for a baby hamster kidney cell line (Reinhardt et al, 1982), for rabbit chondrocytes (Webber et al, 1985), and for rat skin, either in organ culture or in vivo (Kao et al, 1983;Middleton and Pratt, 1978). This property of the triorganotin compounds may very well be the cause of the drastic effects of TBTO on embryonic development of explants of mice in vitro, as was studied recently using limb bud organ cultures (Krowke et al, 1986).…”