Cell Reports volume 4, issue 1, P76-86 2013 DOI: 10.1016/j.celrep.2013.05.039 View full text
Joao Matos, Miguel G. Blanco, Stephen C. West

Abstract: Homologous recombination leads to the formation of DNA joint molecules (JMs) that must be resolved to allow chromosome segregation, but how resolution is temporally coupled with chromosome segregation is unknown. Here, we have analyzed the role of the cell-cycle kinases Cdk and Cdc5 in coordinating these events through their involvement in the phosphoregulation of the Mus81-Mms4 nuclease. By identifying CDC5 and MMS4 mutants that uncouple Mus81-Mms4 activation from cell-cycle progression, we show that JM disen…

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